In this community effort, we compare measurements between 34 laboratories from 19 countries, utilizing mixtures of labelled authentic synthetic standards, to quantify by mass spectrometry four clinically used ceramide species in the NIST (National Institute of Standards and Technology) human blood plasma Standard Reference Material (SRM) 1950, as well as a set of candidate plasma reference materials (RM 8231). Participants either utilized a provided validated method and/or their method of choice. Mean concentration values, and intra- and inter-laboratory coefficients of variation (CV) were calculated using single-point and multi-point calibrations, respectively. These results are the most precise (intra-laboratory CVs ≤ 4.2%) and concordant (inter-laboratory CVs < 14%) community-derived absolute concentration values reported to date for four clinically used ceramides in the commonly analyzed SRM 1950. We demonstrate that calibration using authentic labelled standards dramatically reduces data variability. Furthermore, we show how the use of shared RM can correct systematic quantitative biases and help in harmonizing lipidomics. Collectively, the results from the present study provide a significant knowledge base for translation of lipidomic technologies to future clinical applications that might require the determination of reference intervals (RIs) in various human populations or might need to estimate reference change values (RCV), when analytical variability is a key factor for recall during multiple testing of individuals.
- MeSH
- ceramidy * krev MeSH
- hmotnostní spektrometrie metody MeSH
- kalibrace MeSH
- laboratoře * normy MeSH
- lidé MeSH
- lipidomika metody MeSH
- referenční standardy * MeSH
- reprodukovatelnost výsledků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- referenční standardy * MeSH
- vojenské lékařství normy MeSH
- Geografické názvy
- Česká republika MeSH
Úvod: Medzinárodná iniciatíva pre štandardizáciu diéty pri dysfágii – rámec IDDSI (International Dysphagia Diet Standardization Initiative) je prvým a aktuálne jediným profesionálne uznávaným štandardizovaným systémom pre ľudí s poruchami prehĺtania. Je zameraný na hodnotenie a opis potravín s rôznou úrovňou úpravy textúry a rôzne zahustených nápojov, ako aj na ich testovanie. Na Slovensku je tento systém pomerne neznámy. Cieľ: Cieľom článku je opísať modifikáciu diéty ako jednu z hlavných kompenzačných metód behaviorálnej liečby dysfágie a opísať preklad a jazykovú adaptáciu medzinárodného rámca IDDSI a ich špecifiká. Materiál a metódy: Na základe dohody a súhlasu dobrovoľného prekladateľa a dohody o procese prekladu boli realizované preklad a adaptácia rámca IDDSI a testovacích metód metódou forward-backward translation, s následnou diskusiou s odborníkmi z oblasti jazykovedy, fyziky a klinickej logopédie. Závery: Modifikácia bolusu patrí medzi kompenzačné stratégie behaviorálnej liečby orofaryngeálnej dysfágie. Slovenskú verziu rámca IDDSI možno využiť v klinickej praxi u slovenských pacientov s orofaryngeálnou dysfágiou rôzneho veku. Dodržiavanie tejto diéty môže ľuďom s dysfágiou pomôcť konzumovať bezpečné a zároveň rozmanité a chutné jedlo, a tak zlepšiť ich kvalitu života. Jej implementácia do praxe si vyžaduje medziodborovú komunikáciu a spoluprácu odborníkov, pacientov s dysfágiou a ich rodinných príslušníkov.
Introduction: The International Dysphagia Diet Standardisation Initiative (IDDSI) framework is the first and currently the only professionally recognised standardised system for people with swallowing disorders. It is aimed at evaluating and describing foods with different levels of texture modification and differently thickened beverages, as well as testing them. The system is relatively unknown in Slovakia.
AIM: This study aimed to address the critical need for more accurate growth reference charts for preterm infants, with a particular focus on low- and very low-birth-weight infants. METHODS: The subjects were recruited at a single tertiary centre. The cohort comprised singleton and twin infants born before 37 weeks of gestation, with data collected from 2000 to 2016. Standardised measurements of body parameters were recorded in this mixed longitudinal survey. LMS method was utilised for data analysis. Statistical analysis was performed using SPSS Statistics Version 21. The validation with another new cohort was executed. RESULTS: A total of 1781 infants (52.5% boys) met the inclusion criteria. The median gestational age at birth was 30 weeks, with a median birth weight of 1350 grams. The main findings included the construction of ImaGrow charts for low- and very low-birth-weight infants and significant differences in growth trajectories compared to Fenton+WHO charts. CONCLUSION: Our comprehensive growth references, ImaGrow, are based on a long-term auxological assessment of preterm infants and differ from charts derived from size-at-birth standards or charts for term babies. These charts have significant implications for clinical practice in monitoring and assessing the growth of preterm infants.
- MeSH
- kojenec MeSH
- lidé MeSH
- longitudinální studie MeSH
- novorozenec nedonošený * růst a vývoj MeSH
- novorozenec s velmi nízkou porodní hmotností růst a vývoj MeSH
- novorozenec MeSH
- předškolní dítě MeSH
- referenční hodnoty MeSH
- růstové grafy * MeSH
- Check Tag
- kojenec MeSH
- lidé MeSH
- mužské pohlaví MeSH
- novorozenec MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Edibles are the only source of nutrients and energy for humans. However, ingredients of edibles have undergone many physicochemical changes during preparation and storage. Aging, hydrolysis, oxidation, and rancidity are some of the major changes that not only change the native flavor, texture, and taste of food but also destroy the nutritive value and jeopardize public health. The major reasons for the production of harmful metabolites, chemicals, and toxins are poor processing, inappropriate storage, and microbial spoilage, which are lethal to consumers. In addition, the emergence of new pollutants has intensified the need for advanced and rapid food analysis techniques to detect such toxins. The issue with the detection of toxins in food samples is the nonvolatile nature and absence of detectable chromophores; hence, normal conventional techniques need additional derivatization. Mass spectrometry (MS) offers high sensitivity, selectivity, and capability to handle complex mixtures, making it an ideal analytical technique for the identification and quantification of food toxins. Recent technological advancements, such as high-resolution MS and tandem mass spectrometry (MS/MS), have significantly improved sensitivity, enabling the detection of food toxins at ultralow levels. Moreover, the emergence of ambient ionization techniques has facilitated rapid in situ analysis of samples with lower time and resources. Despite numerous advantages, the widespread adoption of MS in routine food safety monitoring faces certain challenges such as instrument cost, complexity, data analysis, and standardization of methods. Nevertheless, the continuous advancements in MS-technology and its integration with complementary techniques hold promising prospects for revolutionizing food safety monitoring. This review discusses the application of MS in detecting various food toxins including mycotoxins, marine biotoxins, and plant-derived toxins. It also explores the implementation of untargeted approaches, such as metabolomics and proteomics, for the discovery of novel and emerging food toxins, enhancing our understanding of potential hazards in the food supply chain.
- MeSH
- analýza potravin MeSH
- lidé MeSH
- mořské toxiny MeSH
- mykotoxiny * MeSH
- referenční standardy MeSH
- tandemová hmotnostní spektrometrie * metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
