20941100 OR Coherence-controlled holographic microscope Dotaz Zobrazit nápovědu
A coherence-controlled holographic microscope (CCHM) enables quantitative phase imaging with coherent as well as incoherent illumination. The low spatially coherent light induces a coherence gating effect, which makes observation of samples possible also through scattering media. The paper describes theoretically and simulates numerically imaging of a two-dimensional object through a static scattering layer by means of CCHM, with the main focus on the quantitative phase imaging quality. The authors have investigated both strongly and weakly scattering media characterized by different amounts of ballistic and diffuse light. It is demonstrated that the phase information can be revealed also for the case of the static, strongly scattering layer. The dependence of the quality of imaging process on the spatial light coherence is demonstrated. The theoretical calculations and numerical simulations are supported by experimental data gained with a model phase object, as well as living carcinoma cells treated in an optically turbid emulsion.
Východiska: Dlaždicobuněčný karcinom hlavy a krku se vyznačuje lokální invazivitou a tvorbou metastáz do regionálních lymfatických uzlin. V 60 % případů jsou tyto nádory diagnostikovány v pokročilém stadiu a prognóza je nepříznivá. Jedním z důležitých faktorů lokálního, hematogenního či lymfogenního šíření nádoru v lidském těle je migrační schopnost nádorových buněk. Pokročilé mikroskopické metody umožňují nový pohled na buněčnou migraci. Cíl: V tomto článku je prezentována metoda koherencí řízené holografické mikroskopie, která umožňuje neinvazivní kvantitativní hodnocení morfologických a dynamických vlastností živých nádorových buněk. V souvislosti s touto metodou se objevují nové potenciální biomarkery, jejichž význam je však třeba ověřit korelací s klinickými daty.
Background: Squamous cell carcinoma of the head and neck is characterized by local invasiveness and metastases to regional lymph nodes. In 60% of cases, these tumours are diagnosed at an advanced stage, and the prognosis is unfavorable. One of the important factors of local, hematogenous or lymphogenic spread of the tumour in the human body is tumour cells‘ migration ability. Advanced microscopic methods provide a new perspective on cell migration. Purpose: This paper presents a coherence controlled holographic microscopy method that provides a non-invasive quantitative evaluation of morphological and dynamic properties of living tumour cells. In connection with this method, new potential biomarkers are emerging, the significance of which, however, needs to be verified by correlation with clinical data.
- Klíčová slova
- koherencí řízená holografická mikroskopie,
- MeSH
- dlaždicobuněčné karcinomy hlavy a krku * diagnostické zobrazování patologie MeSH
- lidé MeSH
- mikroskopie MeSH
- nádorové biomarkery MeSH
- prognóza MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
Quantitative phase imaging (QPI) brought innovation to noninvasive observation of live cell dynamics seen as cell behavior. Unlike the Zernike phase contrast or differential interference contrast, QPI provides quantitative information about cell dry mass distribution. We used such data for objective evaluation of live cell behavioral dynamics by the advanced method of dynamic phase differences (DPDs). The DPDs method is considered a rational instrument offered by QPI. By subtracting the antecedent from the subsequent image in a time-lapse series, only the changes in mass distribution in the cell are detected. The result is either visualized as a two dimensional color-coded projection of these two states of the cell or as a time dependence of changes quantified in picograms. Then in a series of time-lapse recordings, the chain of cell mass distribution changes that would otherwise escape attention is revealed. Consequently, new salient features of live cell behavior should emerge. Construction of the DPDs method and results exhibiting the approach are presented. Advantage of the DPDs application is demonstrated on cells exposed to an osmotic challenge. For time-lapse acquisition of quantitative phase images, the recently developed coherence-controlled holographic microscope was employed.
- MeSH
- buněčné linie MeSH
- cytologické techniky metody MeSH
- holografie metody MeSH
- krysa rodu rattus MeSH
- mikroskopie metody MeSH
- osmotický tlak fyziologie MeSH
- tvar buňky fyziologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
