Adenosine is a well described anti-inflammatory modulator of immune responses. The aim of the present study was to describe the role of common adenosine agonist 5'-N-ethylcarboxamidoadenosine (NECA) in cytokine production by main porcine T cell subpopulations. TNF-α, IFN-γ, IL-2 and IL-10 were detected by multicolor flow cytometry together with cell surface markers CD3, CD4 and CD8. It was found that NECA inhibits (in a dose-dependent manner) production of pro-inflammatory TNF-α and Th1-associated cytokines IFN-γ, IL-2 in all concanavalin A-stimulated T cell subpopulations. Moreover, production of IL-10 was potentiated in all T cell subpopulations tested. These corresponded well with the fact that all T cell subsets expressed mRNA for adenosine receptor (AR) subtypes to comparable extents. Contrary to concanavalin A-stimulated cells, NECA had a moderate effect on PMA-stimulated T cells, suggesting that AR in pigs acts via signaling pathways not associated with protein-kinase C. Non-selective antagonist CGS15943 as well as allosteric modulator SCH202676 failed to reverse the effect of NECA in pigs. In conclusion, NECA has an anti-inflammatory effect on porcine T cell subpopulations.

• MeSH
• adenosin-5'-(N-ethylkarboxamid) farmakologie   MeSH
• adenosin agonisté   fyziologie   MeSH
• antigeny CD3 fyziologie   MeSH
• CD4-pozitivní T-lymfocyty účinky léků   fyziologie   MeSH
• CD8-pozitivní T-lymfocyty účinky léků   fyziologie   MeSH
• chinazoliny farmakologie   MeSH
• interferon gama biosyntéza   fyziologie   MeSH
• interleukin-10 biosyntéza   fyziologie   MeSH
• interleukin-2 biosyntéza   fyziologie   MeSH
• konkanavalin A farmakologie   MeSH
• prasata imunologie   MeSH
• průtoková cytometrie veterinární   MeSH
• T-lymfocyty - podskupiny účinky léků   fyziologie   MeSH
• T-lymfocyty účinky léků   fyziologie   MeSH
• TNF-alfa biosyntéza   fyziologie   MeSH
• triazoly farmakologie   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• zánět patofyziologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH