Neuroblastoma cell line SH-SY5Y, due to its capacity to differentiate into neurons, easy handling, and low cost, is a common experimental model to study molecular events leading to Alzheimer's disease (AD). However, it is prevalently used in its undifferentiated state, which does not resemble neurons affected by the disease. Here, we show that the expression and localization of amyloid-β protein precursor (AβPP), one of the key molecules involved in AD pathogenesis, is dramatically altered in SH-SY5Y cells fully differentiated by combined treatment with retinoic acid and BDNF. We show that insufficient differentiation of SH-SY5Y cells results in AβPP mislocalization.

• MeSH
• Alzheimerova nemoc metabolismus   MeSH
• amyloidový prekurzorový protein beta metabolismus   MeSH
• biologické modely MeSH
• buněčná diferenciace fyziologie   MeSH
• intravitální mikroskopie metody   MeSH
• lidé MeSH
• mozkový neurotrofický faktor * metabolismus   farmakologie   MeSH
• nádorové buněčné linie MeSH
• neuroblastom MeSH
• neurony fyziologie   MeSH
• oxidační stres MeSH
• proteolýza MeSH
• tretinoin * metabolismus   farmakologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• dopisy MeSH