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Expression and Localization of AβPP in SH-SY5Y Cells Depends on Differentiation State

P. Riegerová, J. Brejcha, D. Bezděková, T. Chum, E. Mašínová, N. Čermáková, SV. Ovsepian, M. Cebecauer, M. Štefl

. 2021 ; 82 (2) : 485-491. [pub] -

Language English Country Netherlands

Document type Letter

Persistent link   https://www.medvik.cz/link/bmc21026256

Neuroblastoma cell line SH-SY5Y, due to its capacity to differentiate into neurons, easy handling, and low cost, is a common experimental model to study molecular events leading to Alzheimer's disease (AD). However, it is prevalently used in its undifferentiated state, which does not resemble neurons affected by the disease. Here, we show that the expression and localization of amyloid-β protein precursor (AβPP), one of the key molecules involved in AD pathogenesis, is dramatically altered in SH-SY5Y cells fully differentiated by combined treatment with retinoic acid and BDNF. We show that insufficient differentiation of SH-SY5Y cells results in AβPP mislocalization.

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$a Neuroblastoma cell line SH-SY5Y, due to its capacity to differentiate into neurons, easy handling, and low cost, is a common experimental model to study molecular events leading to Alzheimer's disease (AD). However, it is prevalently used in its undifferentiated state, which does not resemble neurons affected by the disease. Here, we show that the expression and localization of amyloid-β protein precursor (AβPP), one of the key molecules involved in AD pathogenesis, is dramatically altered in SH-SY5Y cells fully differentiated by combined treatment with retinoic acid and BDNF. We show that insufficient differentiation of SH-SY5Y cells results in AβPP mislocalization.
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$a Brejcha, Jindřich $u Department of Biophysical Chemistry, J. Heyrovský Institute of Physical Chemistry of the Czech Academy of Sciences, Prague, Czech Republic $u Department of Philosophy and History of Science, Faculty of Science, Charles University, Prague, Czech Republic
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$a Bezděková, Dagmar $u Department of Experimental Neurobiology, National Institute of Mental Health, Klecany, Czech Republic $u Department of Psychiatry and Medical Psychology, Third Faculty of Medicine, Charles University, Prague, Czech Republic
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$a Chum, Tomáš $u Department of Biophysical Chemistry, J. Heyrovský Institute of Physical Chemistry of the Czech Academy of Sciences, Prague, Czech Republic
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$a Mašínová, Eva $u Department of Biophysical Chemistry, J. Heyrovský Institute of Physical Chemistry of the Czech Academy of Sciences, Prague, Czech Republic
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$a Čermáková, Nikola $u Department of Biophysical Chemistry, J. Heyrovský Institute of Physical Chemistry of the Czech Academy of Sciences, Prague, Czech Republic
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$a Ovsepian, Saak V $u Department of Experimental Neurobiology, National Institute of Mental Health, Klecany, Czech Republic $u Department of Psychiatry and Medical Psychology, Third Faculty of Medicine, Charles University, Prague, Czech Republic
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$a Cebecauer, Marek $u Department of Biophysical Chemistry, J. Heyrovský Institute of Physical Chemistry of the Czech Academy of Sciences, Prague, Czech Republic
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