Seven- to eight-week-old rabbits were infected with Eimeria intestinalis Cheissin, 1948, a highly immunogenic coccidium, or Eimeria flavescens Marotel et Guilhon, 1941, which is weakly immunogenic. Immune response was investigated at 7, 14 and 21 days post inoculation (DPI). The level of serum immunoglobulins, lymphocyte proliferation stimulated by parasite antigens and weight of mesenteric lymph nodes (MLN) showed similar dynamics in rabbits inoculated with both coccidia species. The amount of serum IgG and IgM, but not IgA, was increased from 14 DPI. The lymphocytes from MLN of infected animals significantly reacted to stimulation with parasite antigen 14 and 21 DPI and MLN were enlarged at 14 DPI. Thus, both parasite species elicited immune response characterized by these parameters in a similar manner despite of their different immunogenicity. The only apparent difference in the responses was in the percentage of CD8+ lymphocytes in the specific site of parasite development (the last third of the small intestine in E. intestinalis, caecum in E. flavescens), which increased in rabbits infected with E. intestinalis but not with E. flavescens. This parameter reflects the status of local immunity and hence the results suggest that the local reaction plays an important role in induction of protective immunity to coccidia in rabbits.
- MeSH
- aktivace lymfocytů MeSH
- CD4-pozitivní T-lymfocyty cytologie imunologie MeSH
- CD8-pozitivní T-lymfocyty cytologie imunologie MeSH
- Eimeria imunologie patogenita MeSH
- ELISA MeSH
- financování organizované MeSH
- kokcidióza imunologie MeSH
- králíci MeSH
- mezenterium imunologie MeSH
- protilátky protozoální krev MeSH
- protozoální infekce imunologie MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
We have developed a PCR assay that in a single reaction distinguishes between Leishmania infantum and Leishmania donovani strains on the basis of different size of the amplicon. The targeted intergenic region between putative biopterin transporter and nucleotide binding protein on chromosome 35 is highly variable, species-specific and can be amplified from clinical samples. Based on the assay, five tested Leishmania archibaldi and L. infantum strains from the Sudan and Ethiopia clearly belong to L. donovani, which is in accordance with a recent multifactorial analysis of these strains. The nucleotide sequence reported in this paper has been submitted to the GenBankTM with accession number EU068004.
- MeSH
- aktivace makrofágů MeSH
- buněčné linie imunologie parazitologie MeSH
- Encephalitozoon cuniculi patogenita růst a vývoj účinky léků MeSH
- financování organizované MeSH
- interferon gama farmakologie MeSH
- peritoneální makrofágy imunologie účinky léků MeSH
- protilátky protozoální MeSH
- techniky in vitro MeSH
- MeSH
- Cyprinidae MeSH
- hlístice klasifikace MeSH
- nemoci ryb diagnóza parazitologie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- MeSH
- Camallanina izolace a purifikace klasifikace patogenita MeSH
- hlístice klasifikace patogenita MeSH
- nemoci ryb diagnóza parazitologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Geografické názvy
- Nová Kaledonie MeSH
