In this study, chromosomal imbalances in tumor tissues (lymphomas) and nucleotide changes in tumor suppressor TP53 were studied in a Bernese Mountain dog bitch and a cross breed bitch. Using comparative genomic hybridization, numerous chromosomal rearrangements were detected, which indicated the heterogeneity in tumor growth: in the cross breed bitch, a deletion on the chromosome 9, and duplications on chromosomes 5, 8 and 17 have been found. In the Bernese Mountain Dog bitch, losses on chromosomes 1, 5, 8, 12, 18, 22, 27, 29 and gains on chromosomes 1, 2, 9, 11, 15, 16, 18, 20, 23, 24, 25, 28, 29, 30, 34, 36, 37 and 38 were identified. With the sequencing of the TP53 gene, one silent mutation, transition A/G at position 138 in exon 5 was detected, without changing the amino acid.
- MeSH
- chromozomální aberace * MeSH
- chromozomy genetika MeSH
- DNA genetika MeSH
- exony genetika MeSH
- genová přestavba genetika MeSH
- geny p53 genetika MeSH
- lymfom genetika patologie veterinární MeSH
- mutace MeSH
- nemoci psů genetika MeSH
- polymerázová řetězová reakce metody MeSH
- psi MeSH
- sekvenční analýza DNA MeSH
- srovnávací genomová hybridizace metody MeSH
- zvířata MeSH
- Check Tag
- psi MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
