The entire double-stranded DNA genome of the Streptomyces aureofaciens phage mu1/6 was sequenced and analyzed. Its size is 38.194 kbp with an overall molar G+C content of 71.2 %. Fifty-two potential open reading frames (orfs) were identified, divided into two oppositely transcribed regions. In the left arm of the mu1/6 genome, an identified putative integrase and possible regulation proteins were identified. The rightwards transcribed region contains genes organized into apparently four functional units responsible for: (i) replication, (ii) DNA packaging and head assembly, (iii) tail morphogenesis, and (iv) lysis. Putative functions were assigned to twelve orfs based on bioinformatic analysis or experimental substantiation. Comparative analysis with three complete genomes of streptomycete phages revealed resemblance with respect to the organization of their genes into functional modules. Closer relationship was observed only between mu1/6 and S. venezuelae phage VWB.
- MeSH
- bakteriofágy genetika imunologie MeSH
- finanční podpora výzkumu jako téma MeSH
- genomová knihovna MeSH
- komponenty genomu genetika MeSH
- lyzogenie genetika MeSH
- otevřené čtecí rámce genetika MeSH
- Siphoviridae genetika izolace a purifikace MeSH
- Streptomyces aureofaciens genetika izolace a purifikace MeSH
- tetracyklin farmakologie izolace a purifikace MeSH
- MeSH
- DNA restrikčně-modifikační enzymy izolace a purifikace klasifikace MeSH
- finanční podpora výzkumu jako téma MeSH
- hospodářská zvířata mikrobiologie MeSH
- plazmidy diagnostické užití izolace a purifikace MeSH
- restrikční enzymy izolace a purifikace klasifikace MeSH
- Staphylococcus aureus enzymologie MeSH
- Streptococcus agalactiae enzymologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
