Interleukin-6 (IL-6) plays an important role in regulation of intestinal inflammatory processes in inflammatory bowel disease (IBD). The levels of IL-6 in media from cultured biopsy samples were determined by ELISA in 14 Crohn's disease (CD) patients, 17 patients with ulcerative colitis (UC), and 24 healthy controls in terminal ileum, cecum, and rectum. Results were confirmed by measuring mRNA expression in selected patients. In CD patients, there were increased levels of IL-6 (expressed in picograms per milligram of biopsy tissue mass) in terminal ileum compared with controls (median, 617 vs. 90.4; p < 0.001). High IL-6 levels were found in the rectum of CD patients with active disease but normal endoscopic findings (791 vs. 131; p < 0.05). This result was confirmed by mRNA expression. There was a substantial increase of IL-6 levels in cultured cecal (median, 327 vs. 94.0; p < 0.001) and rectal mucosa (median, 282 vs.131; p < 0.05) but not in ileal mucosa of UC patients. In conclusion, IL-6 production was higher in IBD patients than in controls; it correlated with disease activity and varied among different intestinal segments. In clinically active CD patients without rectal involvement, high IL-6 levels in cultured rectal mucosa suggest immune stimulation even in the absence of macroscopic inflammation.
- MeSH
- Crohnova nemoc imunologie metabolismus patologie MeSH
- dospělí MeSH
- ELISA MeSH
- interleukin-6 biosyntéza imunologie MeSH
- kultivované buňky MeSH
- lidé středního věku MeSH
- lidé MeSH
- messenger RNA biosyntéza MeSH
- polymerázová řetězová reakce MeSH
- střevní sliznice imunologie metabolismus patologie MeSH
- ulcerózní kolitida imunologie metabolismus patologie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
OBJECTIVE: It is an open question whether multifunctional galectin-3 can be a serum marker in inflammatory bowel disease. METHODS: Western blots and commercial ELISA detected and quantitated the lectin immunocytochemistry using double labeling localized it in tissue sections. RESULTS: Serum concentrations were significantly increased in specimen of patients with active and remission-stage ulcerative colitis and Crohn's disease, associated with emerging positivity of CD14(+) cells. CONCLUSION: Enhanced concentration of galectin-3 in serum reflects presence of disease and points to its involvement in the pathogenesis.
- MeSH
- antigeny CD14 analýza metabolismus MeSH
- biologické markery MeSH
- Crohnova nemoc krev MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- Escherichia coli metabolismus MeSH
- fluorescein-5-isothiokyanát MeSH
- fluorescenční barviva MeSH
- galektin 3 krev MeSH
- idiopatické střevní záněty diagnóza chemicky indukované krev MeSH
- imunohistochemie MeSH
- kolitida chemicky indukované MeSH
- kolon metabolismus MeSH
- lektiny metabolismus MeSH
- lidé MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- síran dextranu MeSH
- ulcerózní kolitida krev MeSH
- western blotting MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
Dysregulation of innate and adaptive intestinal immune responses to bacterial microbiota is supposed to be involved in pathogenetic mechanisms of inflammatory bowel diseases (IBDs). We investigated expression of Toll-like receptor 2 (TLR2), TLR4, and their transmembrane coreceptor CD14 in biopsy samples from patients with IBD and in non-inflamed gut mucosa from controls. Small intestine and colon samples were obtained by colonoscopy from patients with Crohn's disease (CD), ulcerative colitis (UC), and controls. Immunohistochemical analysis of cryostat sections using polyclonal and monoclonal antibodies specific for TLR2, TLR4, and CD14 showed a significant increase in TLR2 expression in the terminal ileum of patients with inactive and active UC against controls. Significant upregulation of TLR4 expression relative to controls was found in the terminal ileum and rectum of UC patients in remission and in the terminal ileum of CD patients with active disease. CD14 expression was upregulated in the terminal ileum of CD patients in remission and with active disease, in the cecum of UC patients in remission and with active disease, and in rectum of UC patients with active disease. Hence, dysregulation of TLR2, TLR4, and CD14 expression in different parts of the intestinal mucosa may be crucial in IBD pathogenesis.
- MeSH
- antigeny CD14 biosyntéza MeSH
- biopsie MeSH
- cékum metabolismus patologie MeSH
- Crohnova nemoc metabolismus patologie MeSH
- financování organizované MeSH
- ileum metabolismus patologie MeSH
- imunohistochemie MeSH
- lidé MeSH
- rektum metabolismus patologie MeSH
- střevní sliznice metabolismus patologie MeSH
- toll-like receptor 2 biosyntéza MeSH
- toll-like receptor 4 biosyntéza MeSH
- ulcerózní kolitida metabolismus patologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
- Publikační typ
- abstrakt z konference MeSH
- MeSH
- dextrany škodlivé účinky MeSH
- Escherichia coli enzymologie imunologie MeSH
- financování vládou MeSH
- gastrointestinální trakt enzymologie imunologie MeSH
- kolitida chemicky indukované MeSH
- Lactobacillus casei enzymologie imunologie MeSH
- myši inbrední BALB C imunologie mikrobiologie MeSH
- probiotika terapeutické užití MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
