BACKGROUND: Myelodysplastic syndrome with isolated chromosome 5q deletion (5q- syndrome) is a clonal stem cell disorder characterized by ineffective hematopoiesis. MicroRNAs (miRNAs) are important regulators of hematopoiesis and their aberrant expression was detected in some clonal hematopoietic disorders. We thus analyzed miRNA expressions in bone marrow CD34+ cells of 5q- syndrome patients. Further, we studied gene expressions of miR-143, miR-145, miR-378 and miR-146a mapped within the 5q deletion. RESULTS: Using microarrays we identified 21 differently expressed miRNAs in 5q- patients compared to controls. Especially, miR-34a was markedly overexpressed in 5q- patients, suggesting its role in an increased apoptosis of bone marrow progenitors. Out of four miRNAs at del(5q), only miR-378 and miR-146a showed reduced gene expression in the patients. An integrative analysis of mRNA profiles and predicted putative targets defined potential downstream targets of the deregulated miRNAs. The list of targets included several genes that play an important role in the regulation of hematopoiesis (e.g. KLF4, LEF1, SPI1). CONCLUSIONS: The study demonstrates global overexpression of miRNAs is associated with 5q- phenotype. Identification of hematopoiesis-relevant target genes indicates that the deregulated miRNAs may be involved in the pathogenesis of 5q- syndrome by a modulation of these targets. The expression data on miRNAs at del(5q) suggest the presence of mechanisms for compensation of a gene dosage.
- MeSH
- antigeny CD34 biosyntéza genetika MeSH
- chromozomální delece MeSH
- lidé MeSH
- lidské chromozomy, pár 5 genetika metabolismus MeSH
- makrocytární anemie genetika metabolismus MeSH
- mikro RNA biosyntéza genetika MeSH
- myelodysplastické syndromy genetika metabolismus MeSH
- stanovení celkové genové exprese MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Twenty-five bifidobacteria were isolated from feces of calves. Isolates were identified, and their functional properties and antimicrobial activity were determined. From 10 strains with suitable properties rifampicin-resistant mutants (RRBs) were prepared and mixture of RRBs was administered to 2-d-old calves. These strains were identified by sequencing as Bifidobacterium animalis ssp. animalis (6 strains), B. thermophilum (2 strains), B. choerinum (1 strain) and B. longum ssp. suis (1 strain). The control group was without probiotic treatment. Survival ability of administered bifidobacteria was monitored in fecal samples by cultivation on modified TPY agar supplemented with mupirocin, acetic acid, and rifampicin. Administered bifidobacteria survived in gastrointestinal tract of calves for at least 60 d. Other bacteria were also determined after cultivation using fluorescence in situ hybridization (FISH). Bifidobacteria and lactobacilli dominated in fecal microflora. Significantly lower amounts of E. coli and higher amounts of bifidobacteria and total anaerobes were found in the treated group relative to the control group.
- MeSH
- antibakteriální látky farmakologie MeSH
- Bacteria izolace a purifikace klasifikace MeSH
- Bifidobacterium fyziologie metabolismus účinky léků MeSH
- časové faktory MeSH
- DNA bakterií genetika MeSH
- feces mikrobiologie MeSH
- financování organizované MeSH
- fylogeneze MeSH
- gastrointestinální trakt mikrobiologie MeSH
- hybridizace in situ MeSH
- mikrobiální testy citlivosti MeSH
- mikrobiální viabilita MeSH
- počet mikrobiálních kolonií MeSH
- probiotika aplikace a dávkování izolace a purifikace MeSH
- shluková analýza MeSH
- skot mikrobiologie MeSH
- techniky typizace bakterií MeSH
- zvířata MeSH
- Check Tag
- skot mikrobiologie MeSH
- zvířata MeSH
The presence of raffinose series oligosaccharides (RSO) was determined by an enzymic method in three commercially available chicken feed mixtures. All feed mixtures contained RSO at a concentration of 2.1-2.2%. Soya meal was identified as the exclusive source of RSO. Subsequently, the bifidogenic effect of stachyose (main soya bean RSO) was also assigned on the growth of poultry intestinal bifidobacteria. Bifidobacteria were counted in chicken intestinal tract using cultivation and FISH methods. Four out of 6 bifidobacterial strains tested grew significantly better on stachyose than on glucose. It can be thus concluded that chicken feed mixtures naturally contain prebiotic oligosaccharides in the form of RSO in higher levels (>2%) compared with the concentration (usually up to 1%) recommended for artificially added prebiotics. Our results therefore indicate that there is no reason for the supplementation of chicken feed mixtures with prebiotics with bifidogenic properties.
- MeSH
- Bifidobacterium izolace a purifikace MeSH
- dietní sacharidy MeSH
- financování organizované MeSH
- hybridizace in situ fluorescenční MeSH
- krmivo pro zvířata MeSH
- kur domácí MeSH
- oligosacharidy analýza MeSH
- počet mikrobiálních kolonií MeSH
- střeva mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
Resistance or susceptibility of bifidobacteria to lysozyme and growth of bifidobacteria in human milk were tested. Susceptible bifidobacterial strains stopped their growth almost immediately after the addition of lysozyme (400 microg/ml), moderately susceptible strains exhibited reduced growth rate, and growth curves of resistant strains were not affected. Strains of human origin were more resistant to lysozyme than animal strains. While strains of B. bifidum grew well in human milk samples, the growth B. animalis strains was inhibited after inoculation to human milk. The resistance to lysozyme seems to be a promising criterion for the selection of new probiotic bifidobacterial strains.
- MeSH
- bakteriální léková rezistence účinky léků MeSH
- Bifidobacterium účinky léků růst a vývoj MeSH
- lidé MeSH
- mateřské mléko účinky léků mikrobiologie MeSH
- mikrobiální testy citlivosti MeSH
- muramidasa farmakologie MeSH
- novorozenec MeSH
- probiotika MeSH
- Check Tag
- lidé MeSH
- novorozenec MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The "cocktail" of human origin rifampicin-resistant bifidobacteria (RRBs) and RRBs from commercial products was administrated to 9 volunteers aged from 22 to 46 years and the survival ability in gastrointestinal tract of these strains was determined. Bifidobacteria represented 0-8 % of total anaerobes detected in gastrointestinal tract of volunteers before the administration of probiotic strains. After the administration of probiotics, bifidobacterial counts increased to 16 % of total bacterial counts. RRBs formed 9-44 % of total counts of bifidobacteria. Then, the counts of RRBs decreased at day 7 after administration, and they were not detected after 14 d. In our study, suitable probiotic bifidobacterial strains for human should be chosen on the basic of in vitro test but the results showed that no strain was able to colonize human tract permanently.
- MeSH
- Bifidobacterium fyziologie MeSH
- dospělí MeSH
- experimenty na lidech MeSH
- financování organizované MeSH
- gastrointestinální trakt mikrobiologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mikrobiální viabilita MeSH
- počet mikrobiálních kolonií MeSH
- probiotika aplikace a dávkování MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
