Within the group of higher fungi, edible medicinal mushrooms have a long history of being used as food and in folk medicine. These species contain biologically active substances with many potential beneficial effects on human health. The Pleurotus genus is representative of medicinal mushrooms because Pleurotus ostreatus is one of the most commonly cultivated culinary mushrooms. In our study, we focused on lesser-known species in the genus Pleurotus and measured their antioxidant and anti-inflammatory activity. We prepared extracts of the mushrooms and analyzed them using HPLC-HRMS, GC-MS, and 1H-NMR. Significant differences in biological activities were found among the Pleurotus spp. extracts. A MeOH extract of P. flabellatus was the most active as a radical scavenger with the highest ORAC, while a chloroform extract had significant anti-inflammatory COX-2 activity. The 80% MeOH extract of P. flabellatus contained the highest amounts of ergosterol, ergothioneine, and mannitol. The 80% MeOH extract of P. ostreatus Florida was the most active in the NF-κB inhibition assay and had the highest content of β-glucans (43.3% by dry weight). Given the antioxidant and anti-inflammatory properties of P. flabellatus, the potential therapeutic usefulness of this species is worth evaluating through in-depth investigations and confirmation by clinical trials.
- Publikační typ
- časopisecké články MeSH
The aim of this work was to prepare a softwood substrate on which to grow edible and medicinal mushrooms. Liquid digestate from a biogas station was successfully used in spruce sawdust fermentation. Pleurotus ostreatus, P. eryngii, and Ganoderma lucidum were grown on the obtained substrates and their mycelia grew at rates similar to rates of growth on control beech sawdust; values ranged from 4.1 to 5.54 mm/day. A 6-week fermentation period was determined to be sufficient for removing volatile extractives from sawdust (76% removal efficiency), which elevated content was shown to be most critical for fungal growth. Removal of 47% of resinous compounds and a decrease in the carbon-to-nitrogen ratio in the growth substrate were found during sawdust fermentation in the presence of the liquid digestate. Among ligninolytic enzymes, the growth substrates produced here favored laccase produced by tested fungi. It follows that utilizing wastes from biogas production to reuse softwood wastes could make an environmentally friendly and economically viable biotechnology for producing mushrooms.
Oyster mushrooms are an interesting source of biologically active glucans and other polysaccharides. This work is devoted to the isolation and structural characterization of polysaccharides from basidiocarps of the cultivated oyster mushroom, Pleurotus ostreatus. Five polysaccharidic fractions were obtained by subsequent extraction with cold water, hot water and two subsequent extractions with 1 m sodium hydroxide. Branched partially methoxylated mannogalactan and slightly branched (1→6)-β-d-glucan predominated in cold- and hot-water-soluble fractions, respectively. Alternatively, these polysaccharides were obtained by only hot water extraction and subsequent two-stage chromatographic separation. The alkali-soluble parts originating from the first alkali extraction were then fractionated by dissolution in dimethyl sulfoxide (DMSO). The polysaccharide insoluble in DMSO was identified as linear (1→3)-α-d-glucan, while branched (1→3)(1→6)-β-d-glucans were found to be soluble in DMSO. The second alkaline extract contained the mentioned branched β-d-glucan together with some proteins. Finally, the alkali insoluble part was a cell wall complex of chitin and β-d-glucans.
- MeSH
- chemická frakcionace MeSH
- chromatografie MeSH
- fungální polysacharidy chemie izolace a purifikace MeSH
- fytonutrienty chemie izolace a purifikace MeSH
- glukany chemie MeSH
- molekulární struktura MeSH
- monosacharidy chemie MeSH
- Pleurotus chemie MeSH
- plodnice hub chemie MeSH
- spektrální analýza MeSH
- Publikační typ
- časopisecké články MeSH
A 120-day experiment was conducted to compare the removal of polycyclic aromatic hydrocarbons (PAHs) from agricultural soil after natural attenuation (NA), phytoremediation (P), mycoremediation (M), and plant-assisted mycoremediation (PAM) approaches in relation to the extracellular enzyme activities in soil. The NA treatment removed the total soil PAH content negligibly. The P treatment using maize (Zea mays) enhanced only the removal of low and medium molecular PAHs. The Pleurotus ostreatus cultivated on 30-50 mm wood chip substrate used in M treatment was the most successful in the removal of majority PAHs. Therefore, significantly (p < 0.05) highest total PAH removal by 541.4 μg/kg dw (dry weight) (36%) from all tested M treatments was observed. When using the same fungal substrate together with maize in PAM treatment, the total PAH removal was not statistically different from the previous M treatment. However, the maize-assisted mycoremediation treatment significantly boosted fungal biomass, microbial and manganese peroxidase activity in soil which strongly correlated with the removal of total PAHs. The higher PAH removal in that PAM treatment could be reflected in the following post-harvest time. Our suggested M and PAM approaches could be promising in situ bioremediation strategies for PAH-contaminated soils.
- MeSH
- biodegradace MeSH
- biomasa MeSH
- extracelulární prostor enzymologie MeSH
- kukuřice setá cytologie metabolismus MeSH
- látky znečišťující půdu izolace a purifikace metabolismus MeSH
- peroxidasy metabolismus MeSH
- Pleurotus cytologie metabolismus MeSH
- polycyklické aromatické uhlovodíky izolace a purifikace metabolismus MeSH
- půda chemie MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
