Tick-borne encephalitis (TBE) is a vector-borne infection associated with a variety of potentially serious complications and sequelae. Vaccination against TBE is strongly recommended for people living in endemic areas. There are two TBE vaccination schemes - standard and rapid - which differ in the onset of protection. With vaccination in a rapid schedule, protection starts as early as 4 weeks after the first dose and is therefore especially recommended for non-immune individuals travelling to endemic areas. Both schemes work reliably in immunocompetent individuals, but only little is known about how TBE vaccination works in people with HIV infection. Our aim was to assess the immunogenicity and safety of the rapid scheme of TBE vaccination in HIV-1 infected individuals. Concentrations of TBE-specific IgG > 126 VIEU/ml were considered protective. The seroprotection rate was 35.7% on day 28 and 39.3% on day 60. There were no differences between responders and non-responders in baseline and nadir CD4 + T lymphocytes. No serious adverse events were observed after vaccination. The immunogenicity of the TBE vaccination was unsatisfactory in our study and early protection was only achieved in a small proportion of vaccinees. Therefore, TBE vaccination with the rapid scheme cannot be recommended for HIV-1 infected individuals.

• MeSH
• dospělí MeSH
• HIV infekce komplikace   MeSH
• HIV-1 * MeSH
• imunogenicita vakcíny * MeSH
• klíšťová encefalitida prevence a kontrola   MeSH
• lidé MeSH
• očkovací schéma MeSH
• vakcinace * MeSH
• virové vakcíny aplikace a dávkování   imunologie   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

Etoposide is a well-known and widely used anticancer drug that displays several side effects. In addition, tumors often acquire resistance to this drug. Our aim is to develop a combination therapy that would augment toxicity of etoposide in malignant cells. Based on literature and our experiments, we selected mifepristone (RU486) as a potential supporting molecule that is able to enhance etoposide toxicity against cancer cells. All experiments were performed with Hep G2 cells, a well-known and described human hepatocellular carcinoma cell line. By using xCELLigence system, we demonstrated that mifepristone enhances toxicity of etoposide in a dose dependent manner with concomitant caspase-3 activity. We evaluated upregulation of Bax because mifepristone was demonstrated to modulate proapoptotic Bax protein expression. Our data show only weak and not statistically significant increase of Bax expression. On the other hand, we show that mifepristone increases etoposide toxicity via inhibition of ABC transporters, coupled with significant increase of intracellular etoposide concentration. In conclusion, we demonstrate that mifepristone has a synergistic effect with etoposide treatment in the Hep G2 cells and that the effect is related to ABC transporters inhibition.

• MeSH
• ABC transportér z rodiny G, člen 2 antagonisté a inhibitory   MeSH
• antitumorózní látky fytogenní farmakologie   MeSH
• biologický transport účinky léků   MeSH
• buňky Hep G2 MeSH
• buňky K562 MeSH
• etoposid farmakologie   MeSH
• kaspasa 3 metabolismus   MeSH
• lidé MeSH
• mifepriston farmakologie   MeSH
• nádorové proteiny antagonisté a inhibitory   MeSH
• P-glykoproteiny antagonisté a inhibitory   MeSH
• protein X asociovaný s bcl-2 metabolismus   MeSH
• synergismus léků MeSH
• viabilita buněk účinky léků   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

• Publikační typ
• abstrakty MeSH

• MeSH
• financování organizované MeSH
• Publikační typ
• abstrakty MeSH

• MeSH
• aromatické hydroxylasy metabolismus   MeSH
• cytochrom P-450 CYP1A2 metabolismus   MeSH
• finanční podpora výzkumu jako téma MeSH
• glykosidy farmakologie   chemie   MeSH
• hepatocyty enzymologie   účinky léků   MeSH
• lidé MeSH
• N-demethylasy metabolismus   MeSH
• regulace genové exprese enzymů účinky léků   MeSH
• silymarin farmakologie   chemie   MeSH
• Check Tag
• lidé MeSH

• MeSH
• komunitní zdravotní střediska organizace a řízení   zásobování a distribuce   MeSH
• oblast s nedostatečnou zdravotní péčí MeSH
• pomocní ošetřovatelé zásobování a distribuce   MeSH
• pomocný zdravotnický personál zásobování a distribuce   MeSH
• porodní asistentky zásobování a distribuce   MeSH
• venkovské obyvatelstvo MeSH
• zdraví venkovských oblastí MeSH
• zdravotnické služby pro venkov organizace a řízení   pracovní síly   MeSH
• zubní technici zásobování a distribuce   MeSH