N6-methyladenosine (m6A) and N6,2'-O-dimethyladenosine (m6Am) are two abundant modifications found in mRNAs and ncRNAs that can regulate multiple aspects of RNA biology. They function mainly by regulating interactions with specific RNA-binding proteins. Both modifications are linked to development, disease and stress response. To date, three methyltransferases and two demethylases have been identified that modify adenosines in mammalian mRNAs. Here, we present a comprehensive analysis of the interactomes of these enzymes. PCIF1 protein network comprises mostly factors involved in nascent RNA synthesis by RNA polymerase II, whereas ALKBH5 is closely linked with most aspects of pre-mRNA processing and mRNA export to the cytoplasm. METTL16 resides in subcellular compartments co-inhabited by several other RNA modifiers and processing factors. FTO interactome positions this demethylase at a crossroad between RNA transcription, RNA processing and DNA replication and repair. Altogether, these enzymes share limited spatial interactomes, pointing to specific molecular mechanisms of their regulation.
- MeSH
- adaptorové proteiny signální transdukční genetika metabolismus MeSH
- adenosin analogy a deriváty metabolismus MeSH
- alfa-ketoglutarát-dependentní dioxygenasa, AlkB homolog 5 genetika metabolismus MeSH
- anotace sekvence MeSH
- gen pro FTO genetika metabolismus MeSH
- genetická transkripce MeSH
- genová ontologie MeSH
- HEK293 buňky MeSH
- jaderné proteiny genetika metabolismus MeSH
- lidé MeSH
- mapování interakce mezi proteiny MeSH
- messenger RNA genetika metabolismus MeSH
- methyltransferasy genetika metabolismus MeSH
- N-demethylasy genetika metabolismus MeSH
- nekódující RNA genetika metabolismus MeSH
- oprava DNA MeSH
- protein - isoformy genetika metabolismus MeSH
- replikace DNA MeSH
- vazba proteinů MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- aromatické hydroxylasy metabolismus MeSH
- cytochrom P-450 CYP1A2 metabolismus MeSH
- finanční podpora výzkumu jako téma MeSH
- glykosidy farmakologie chemie MeSH
- hepatocyty enzymologie účinky léků MeSH
- lidé MeSH
- N-demethylasy metabolismus MeSH
- regulace genové exprese enzymů účinky léků MeSH
- silymarin farmakologie chemie MeSH
- Check Tag
- lidé MeSH
