The intestinal microbiota has enormous impact on the health and performance of horses. Staphylococci belong in the phylum Firmicutes, and their occurrence, especially of methicillin-resistant strains and species, has been reported in horses previously. Moreover, biofilm formation is one of the virulence factors; it has been not completely studied in fecal coagulase-negative staphylococci (CoNS) from horses. Therefore, this study was focused on biofilm formation by various species of fecal CoNS from horses because it has been never reported before. In addition, their antibiotic profile was tested. Horses (42) of various breeds from Slovakia/Poland were sampled. Variability in the species of CoNS was detected in feces of horses. Thirty-two strains were identified by using the MALDI-TOF system and classified into nine species and three subspecies of CoNS: Staphylococcus capitis, S. cohnii subsp. cohnii, S. cohnii subsp. urealyticus, S. cohnii subsp. casei, S. epidermidis, S. haemolyticus, S. pasteuri, S. sciuri, S. vitulinus, S. warneri, and S. xylosus. The most frequent species was S. vitulinus. Twenty-two strains showed high biofilm production; 10 strains showed low-grade biofilm production. The highest biofilm formation was measured in the species S. xylosus. Eleven strains (of 32) were methicillin-resistant; the others were susceptible to methicillin.
- MeSH
- antibakteriální látky farmakologie MeSH
- biofilmy růst a vývoj MeSH
- druhová specificita MeSH
- feces mikrobiologie MeSH
- koagulasa nedostatek MeSH
- koně MeSH
- kyselina mléčná metabolismus MeSH
- mikrobiální testy citlivosti MeSH
- rezistence na methicilin MeSH
- stafylokokové infekce epidemiologie mikrobiologie veterinární MeSH
- Staphylococcus klasifikace účinky léků izolace a purifikace fyziologie MeSH
- střevní mikroflóra MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Polsko MeSH
- Slovenská republika MeSH
The impact of probiotic supplementation of canine-derived strain Lactobacillus fermentum AD1-CCM7421 in freeze-dried form on quantitative composition of microbiota and short-chain fatty acid profile in feces of dogs was demonstrated by two independent studies (straightforward repeated-measures model; study I: a dose of 2 g per dog for 2 weeks, 10(8) CFU/g, n = 12; study II: 1 g per dog for 1 week, 10(7) CFU/g, n = 11. The results revealed a significant increase of lactic acid bacteria population persisting also after the cessation of probiotic application in both studies. A reduction of clostridia (study I, p (sum) < 0.01) and tested Gram-negative bacterial genera (coliforms, Aeromonas sp., Pseudomonas sp., study II, p < 0.05) was also detected. The strain AD1-CCM7421 colonized the canine digestive tract in sufficient numbers (10(5)-10(6) CFU/g) and it persisted in the majority of dogs after cessation of probiotic application. An increase of short-chain fatty acid concentrations (study I: butyric, succinic, valeric, formic acid) especially in the early post-treatment phase (p < 0.05) most likely led to a decrease of fecal pH value (p < 0.05) without negative influence on fecal consistency throughout the studies.
- MeSH
- Bacteria klasifikace genetika izolace a purifikace MeSH
- feces chemie mikrobiologie MeSH
- gastrointestinální trakt metabolismus mikrobiologie MeSH
- Limosilactobacillus fermentum chemie fyziologie MeSH
- lyofilizace MeSH
- mastné kyseliny metabolismus MeSH
- metagenom MeSH
- nemoci psů metabolismus mikrobiologie prevence a kontrola MeSH
- potravní doplňky analýza MeSH
- probiotika chemie MeSH
- psi MeSH
- zvířata MeSH
- Check Tag
- psi MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Enterococci isolated from 28 different commercially available feeds (10-1000 CFU/mL) were identified and their probiotic potential was determined. Species identification of 22 selected strains was performed by intergenic length-polymorphism analysis (tRNA-PCR); PCR products were analyzed using capillary electrophoresis. Six strains were allotted to the species Enterococcus faecium, four to E. faecalis, one to E. hirae; the remaining strains were not classed. The strains were sensitive to vancomycin, ampicillin, tetracycline and rifampicin. They were able to adhere to human as well as canine intestinal mucus. They produced lactic acid (0.99-1.04 mmol/L) and most of them were urease-positive with sufficient survival in 5 % Oxgall-bile. They did not show any inhibitory activity due to antimicrobial substances. Plasmid DNA was detected in 8 strains, the bands responding to small molecular size (10 kbp). Considering all probiotically important properties, E. faecium strain EE3 was suggested as potential feed additive.
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální proteiny metabolismus MeSH
- Enterococcus chemie izolace a purifikace metabolismus účinky léků MeSH
- krmivo pro zvířata mikrobiologie MeSH
- kyselina mléčná metabolismus MeSH
- lidé MeSH
- probiotika chemie izolace a purifikace MeSH
- psi MeSH
- střeva mikrobiologie MeSH
- ureasa metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- psi MeSH
- zvířata MeSH
- MeSH
- antibakteriální látky imunologie terapeutické užití MeSH
- bakteriální léková rezistence genetika imunologie účinky léků MeSH
- bakteriocinové plazmidy genetika imunologie škodlivé účinky MeSH
- Enterococcus genetika klasifikace účinky léků MeSH
- finanční podpora výzkumu jako téma MeSH
- rezistence na ampicilin genetika imunologie účinky léků MeSH
- rezistence na chloramfenikol genetika imunologie účinky léků MeSH
- rezistence na kanamycin genetika imunologie účinky léků MeSH
- ureasa fyziologie chemie MeSH
- MeSH
- Enterococcus faecalis růst a vývoj MeSH
- finanční podpora výzkumu jako téma MeSH
- lipidy krev MeSH
- nemoci psů mikrobiologie prevence a kontrola MeSH
- obezita prevence a kontrola MeSH
- počet mikrobiálních kolonií MeSH
- probiotika aplikace a dávkování MeSH
- střeva mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- MeSH
- antibakteriální látky farmakologie MeSH
- Enterococcus fyziologie izolace a purifikace MeSH
- extracelulární matrix mikrobiologie MeSH
- fibrinogen metabolismus MeSH
- fibronektiny metabolismus MeSH
- laktoferrin metabolismus MeSH
- lidé MeSH
- transferin metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- MeSH
- bakteriální adheze MeSH
- biochemická analýza krve MeSH
- Enterococcus izolace a purifikace metabolismus účinky léků MeSH
- feces mikrobiologie MeSH
- finanční podpora výzkumu jako téma MeSH
- Lactobacillus izolace a purifikace metabolismus účinky léků MeSH
- mikrobiální testy citlivosti statistika a číselné údaje MeSH
- psi MeSH
- žluč MeSH
- zvířata MeSH
- Check Tag
- psi MeSH
- zvířata MeSH
