Dental caries is a biofilm-related disease, widely perceived to be caused by oral ecological imbalance when cariogenic/aciduric bacteria obtain an ecological advantage. Compared with planktonic bacteria, dental plaques are difficult to remove under extracellular polymeric substance protection. In this study, the effect of caffeic acid phenethyl ester (CAPE) on a preformed cariogenic multi-species biofilm was evaluated, which was comprised of cariogenic bacteria (Streptococcus mutans), commensal bacteria (Streptococcus gordonii), and a pioneer colonizer (Actinomyces naeslundii). Our result revealed that treatment with 0.08 mg/mL CAPE reduced live S. mutans in the preformed multi-species biofilm while not significantly changing the quantification of live S. gordonii. CAPE significantly reduced the production of lactic acid, extracellular polysaccharide, and extracellular DNA and made the biofilm looser. Moreover, CAPE could promote the H2O2 production of S. gordonii and inhibit the expression of SMU.150 encoding mutacin to modulate the interaction among species in biofilms. Overall, our results suggested that CAPE could inhibit the cariogenic properties and change the microbial composition of the multi-species biofilms, indicating its application potential in dental caries prevention and management.

• MeSH
• biofilmy MeSH
• lidé MeSH
• matrix extracelulárních polymerních látek metabolismus   MeSH
• peroxid vodíku * metabolismus   MeSH
• Streptococcus mutans metabolismus   MeSH
• zubní kaz * prevence a kontrola   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

Streptococcus mutans patrí medzi primárnych kolonizátorov ústnej dutiny. Vďaka vhodným virulenčným faktorom – produkcia glukánov, rezistencia voči kyselinám, prirodzená kompetencia a tvorba kompaktného biofilmu, má istú výhodu oproti iným primárnym kolonizátorom. Je považovaný za hlavný etiologický agens zubného kazu. V súčasnosti je zubný kaz považovaný za fenomén súvisiaci s metabolickou aktivitou baktérií v orálnom biofilme a má významný nielen zdravotný, ale aj socioekonomický dopad.

Streptococcus mutans is one of the primary colonizers of the oral cavity. Carriage of the appropriate virulence factors - production of glucans, acid resistance, natural competence, and ability to form compact biofilm, confers a certain advantage to S. mutans over other primary colonizers. It is believed to be the main etiological agent of dental caries. Currently, dental caries seems to be a phenomenon related to the meta­bolic activity of bacteria in the oral biofilm with an impact not only on health but also on socio-economic outcome.

• MeSH
• biofilmy růst a vývoj   MeSH
• extracelulární matrix mikrobiologie   MeSH
• glukany MeSH
• lidé MeSH
• orální hygiena MeSH
• signální transdukce MeSH
• Streptococcus mutans * genetika   metabolismus   patogenita   růst a vývoj   MeSH
• ústní sliznice mikrobiologie   MeSH
• zubní kaz * etiologie   mikrobiologie   patologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• přehledy MeSH

Ginkgo biloba has long been used in traditional Chinese medicine. In this study, ginkgoneolic acid, a kind of compound extracted from G. biloba, was investigated for its effects on growth, acid production, adherence, biofilm formation, and biofilm morphology of Streptococcus mutans. The results showed that ginkgoneolic acid inhibited not only the growth of S. mutans planktonic cells at minimum inhibitory concentration (MIC) of 4 μg/mL and minimum bactericidal concentration (MBC) of 8 μg/mL but also the acid production and adherence to saliva-coated hydroxyapatite of S. mutans at sub-MIC concentration. In addition, this agent was effective in inhibiting the biofilm formation of S. mutans (MBIC(50) = 4 μg/mL), and it reduced 1-day-developed biofilm of S. mutans by 50 % or more at low concentration (MBRC(50) = 32 μg/mL). Furthermore, the present study demonstrated that ginkgoneolic acid disrupted biofilm integrity effectively. These findings suggest that ginkgoneolic acid is a natural anticariogenic agent in that it exhibits antimicrobial activity against S. mutans and suppresses the specific virulence factors associated with its cariogenicity.

• MeSH
• antibakteriální látky izolace a purifikace   farmakologie   MeSH
• bakteriální adheze účinky léků   MeSH
• biofilmy růst a vývoj   MeSH
• Ginkgo biloba chemie   MeSH
• kyseliny karboxylové metabolismus   MeSH
• mikrobiální testy citlivosti MeSH
• mikrobiální viabilita účinky léků   MeSH
• rostlinné extrakty izolace a purifikace   farmakologie   MeSH
• sliny mikrobiologie   MeSH
• Streptococcus mutans účinky léků   růst a vývoj   metabolismus   fyziologie   MeSH
• Publikační typ
• časopisecké články MeSH