Galactooligosaccharides (GOS) are currently attracting considerable interest as prebiotic substances and can be prepared by transgalactosylation reactions from lactose using β-galactosidase. We applied various combinations of the commercial β-galactosidases, such as Nola Fit 5500, Saphera 2600 L, Maxilact LGI 5000 and Maxilact A4 MG to achieve the highest yield of GOS and reduced lactose content. The combination of the Maxilact LGI 5000 and Nola Fit 5500 resulted in amount of GOS 105 g L-1 with lactose content lower than 5 g L-1, whilst the combination of the Maxilact A4 MG and Maxilact LGI 5000 enzymes led to an increase in GOS to 141,1 g L-1 and decrease of the lactose content to 46,9 g L-1. The combination of enzymes produced a higher yield of GOS, reduced the concentration of lactose, eventually, increases the efficiency of galactooligosaccharides purification that could be potentially used in the further investigations.
( GOS) sú ne stráviteľné sacharidy známe ako prebiotiká – môžu stimulovať rast a aktivitu prospešnej črevnej mikroflóry ( bifidobaktérie a laktobacily ) a inhibujú rast potenciálne patogénnych baktérií. GOS sú komerčne vyrábané z laktózy pomocou mikrobiálnej β - galaktozidázy. Tieto enzýmy katalyzujú hydrolýzu , ako aj transgalaktozyláciu laktózy . Pomer medzi týmito reakciami sa líši v závislosti od zdroja enzýmu a podmienok enzýmovej . V poslednom období, inovačné stratégie pre syntézu GOS zahŕňajú imobilizáciu enzýmov a využitie srvátky ako základnej suroviny.
Galacto-oligosaccharides (GOS) are non-digestible carbohydrates know as a prebiotic – can stimulate growth and activity of beneficial intestinal microflora (bifidobacteria and lactobacilli) and inhibit the growth of potencially pathogenic bacteria. GOS are commercially produced from lactose using microbial β -galactosidases. These enzymes catalyze hydrolysis as well as the transgalactosylation of lactose. The proportion between these reactions varies depending on the source of the enzyme and conditions of enzymatic reaction. More recently, an innovative strategies for GOS synthesis are immobilization of enzymes and usage of whey as a source of the lactose.
