- MeSH
- choroidea krevní zásobení MeSH
- fovea centralis anatomie a histologie MeSH
- glymfatický systém fyziologie MeSH
- hematoretinální bariéra anatomie a histologie fyziologie MeSH
- lidé MeSH
- retina * anatomie a histologie diagnostické zobrazování MeSH
- retinální cévy anatomie a histologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
Fluid transport in the perivascular space by the glia-lymphatic (glymphatic) system is important for the removal of solutes from the brain parenchyma, including peptides such as amyloid-beta which are implicated in the pathogenesis of Alzheimer's disease. The glymphatic system is highly active in the sleep state and under the influence of certain of anaesthetics, while it is suppressed in the awake state and by other anaesthetics. Here we investigated whether light sheet fluorescence microscopy of whole optically cleared murine brains was capable of detecting glymphatic differences in sleep- and awake-mimicking anaesthesia, respectively. Using light-sheet imaging of whole brains, we found anaesthetic-dependent cerebrospinal fluid (CSF) influx differences, including reduced tracer influx along tertiary branches of the middle cerebral artery and reduced influx along dorsal and anterior penetrating arterioles, in the awake-mimicking anaesthesia. This study establishes that light sheet microscopy of optically cleared brains is feasible for quantitative analyses and can provide images of the entire glymphatic system in whole brains.
- MeSH
- anestezie MeSH
- arteria cerebri media fyziologie MeSH
- arterioly fyziologie MeSH
- fluorescenční mikroskopie metody MeSH
- glymfatický systém fyziologie MeSH
- mozek ultrastruktura MeSH
- mozkomíšní mok metabolismus MeSH
- mozkový krevní oběh fyziologie MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- neurozobrazování metody MeSH
- spánek fyziologie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
