Several plants have the potential to protect essential reproductive processes such as spermatogenesis or steroidogenesis, however, effective concentrations and main mechanisms of action are still unknown. This in vitro study was aimed to assess the effects of Apium graveolens L., Levisticum officinale, and Calendula officinalis L. extracts on the structural integrity, functional activity and gap junctional intercellular communication (GJIC) in mice Leydig cells. TM3 cells were grown in the presence of experimental extracts (37.5; 75; 150 and 300 μg/ml) for 24 h. For the present study, high-performance liquid chromatography analysis was used to quantify flavonoids or phenolic acids. Subsequently, Leydig cell viability was assessed by alamarBlue assay, while the cell membrane integrity was detected by 5-carboxyfluorescein diacetate-acetoxymethyl ester. The level of steroid hormones production was determined by enzyme-linked immunosorbent assay. Additionally, GJIC was assessed by scalpel loading/dye transfer assay. According to our results, Apium graveolens L. significantly increased the viability and cell membrane integrity at 75 μg/ml (109.0±4.3%) followed by a decline at 300 μg/ml (89.4±2.3%). In case of Levisticum officinale and Calendula officinalis L. was observed significant decrease at 150 μg/ml (88.8±11.66%; 87.4±6.0%) and 300 μg/ml (86.2±9.3%; 84.1±4.6%). Furthermore, Apium graveolens L. significantly increased the progesterone and testosterone production (75 and 150 μg/ml) however, Levisticum officinale and Calendula officinalis L. significantly reduced steroid hormones synthesis at 150 and 300 μg/ml. Finally, the disturbance of GJIC was significantly affected at 300 μg/ml of Levisticum officinale (82.5±7.7%) and Calendula officinalis L. (79.8±7.0%). The balanced concentration ratio may support the Leydig cell function, steroidogenesis as well as all essential parameters that may significantly improve reproductive functions.
- MeSH
- Apium * chemie MeSH
- buněčná membrána účinky léků patologie MeSH
- buněčné linie MeSH
- Leydigovy buňky účinky léků metabolismus patologie MeSH
- libeček * chemie MeSH
- měsíček * chemie MeSH
- mezerový spoj účinky léků metabolismus patologie MeSH
- mezibuněčná komunikace účinky léků MeSH
- myši inbrední BALB C MeSH
- pohlavní steroidní hormony biosyntéza MeSH
- rostlinné extrakty izolace a purifikace farmakologie MeSH
- viabilita buněk účinky léků MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
BACKGROUND: The aim of this work was to compare water and organic extracts, infusions and tinctures from flowers and leaves of Calendula officinalis in terms of their biological activity and composition. The purpose of work was investigation whether the leaves and stems are really the waste or they contain interesting substances which could be utilized. Antimicrobial, antifungal, antioxidant and anti-inflammatory activities were studied. Then, the ability to inhibit collagenase was studied as well. Cytotoxicity was tested for all the samples on mammalian cell lines. METHODS: To determine the composition of extracts, infusions and tinctures phytochemical analysis (the set of colour reactions for the detection of groups of biologically active compounds) was carried out and showed that samples from flowers and leaves contain the same groups of biologically active substances (proteins and amino acids, reducing sugars, flavonoids, saponins, phenolics, terpenoids, steroids, glycosides). The antimicrobial activity of tested samples was proved, where the most sensitive bacterium was Micrococcus luteus and the most sensitive yeast was Geotrichum candidum. RESULTS: The study of anti-collagenase activity has shown that the enzymatic reaction of collagenase was affected by all tested samples and their effect was concentration dependent. Cytotoxicity of water and methanol extracts at cell lines HEK 293T and HepG2 was observed. CONCLUSION: Cells HepG2 were more sensitive than cells HEK 293T. Using cell line RAW 264.7, antiinflammatory activity of all samples was observed. Tincture of leaves was the most effective.
- MeSH
- antiflogistika izolace a purifikace toxicita MeSH
- antiinfekční látky izolace a purifikace toxicita MeSH
- antioxidancia izolace a purifikace toxicita MeSH
- buněčné linie MeSH
- buňky Hep G2 MeSH
- HEK293 buňky MeSH
- květy chemie MeSH
- lidé MeSH
- listy rostlin chemie MeSH
- měsíček chemie MeSH
- rostlinné extrakty izolace a purifikace toxicita MeSH
- viabilita buněk účinky léků MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Mezi významná rostlinná antiflogistika patří Calendulae flos. Řada odborných studií prokázala, že nejdůležitějšími protizánětlivými látkami této drogy jsou pentacyklické triterpendiolové estery. Nejúčinnější je především faradiolmonoester, jehož obsah by mohl sloužit jako vhodný parametr pro hodnocení kvality přípravků s?obsahem měsíčku lékařského.
- MeSH
- antiflogistika farmakokinetika farmakologie terapeutické užití MeSH
- antitumorózní látky farmakokinetika farmakologie terapeutické užití MeSH
- estery farmakokinetika farmakologie terapeutické užití MeSH
- financování organizované MeSH
- kvetoucí vrcholky rostlin MeSH
- měsíček chemie růst a vývoj účinky léků MeSH
- parasympatolytika farmakokinetika farmakologie terapeutické užití MeSH
- péče o kůži metody využití MeSH
- triterpeny farmakokinetika farmakologie terapeutické užití MeSH
