ETHNOPHARMACOLOGICAL RELEVANCE: Different plants are used for the treatment of various ailments and Acorus calamus L. is one such plant found in Western Himalaya. Rhizome of this plants has ethnomedicinal significance, as its rhizome is used for curing fever, pain and inflammation. An attempt has been made to alter the phytochemicals and increase its antioxidant property in a sustainable way with the help of mycorrhizal inoculation. AIM OF THE STUDY: Study of mycorrhizal (Funneliformis mosseae) impact on the biological activities and phytochemical profile of A. calamus L. rhizome and in silico studies of phytochemicals for their anti-inflammatory property. MATERIALS AND METHODS: F. mosseae was mass multiplied by single spore culture and then A. calamus rhizomes were inoculated with it. Antioxidant potential of rhizome extract was observed by DPPH and FRAP assays and the phytochemical profiling was done with GC-MS analysis. For observing antimicrobial activity disc diffusion method was employed. Dominant phytochemicals α-asarone and monolinolein TMS were chosen for molecular docking studies against four receptors (4COX, 2AZ5, 5I1B, 1ALU). RESULTS: There was increase in antioxidant activity of rhizome extract after mycorrhizal inoculation. However, no change in antimicrobial activity was observed in the plant after mycorrhizal inoculation. The comparison in phytochemicals was observed by GC-MS analysis which showed qualitative and quantitative variation in biochemical content in plants. The phytochemical, α-asarone and monolinolein TMS showed highest docking score and least binding energy against 1ALU and 4COX respectively for anti-inflammatory activity. CONCLUSION: Medicinal plants are potential source of antioxidants which can be increased by mycorrhizal inoculation without addition of chemical fertilizers and also results in altering the phytochemical composition.
- MeSH
- antiflogistika izolace a purifikace farmakologie MeSH
- antiinfekční látky izolace a purifikace farmakologie MeSH
- antioxidancia izolace a purifikace farmakologie MeSH
- diskové difúzní antimikrobiální testy MeSH
- fytonutrienty izolace a purifikace farmakologie MeSH
- houby fyziologie MeSH
- mykorhiza fyziologie MeSH
- oddenek MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- počítačová simulace MeSH
- puškvorec chemie mikrobiologie MeSH
- rostlinné extrakty farmakologie MeSH
- simulace molekulového dockingu MeSH
- tradiční lékařství MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Indie MeSH
Members of Hymenochaetaceae fungi are among well-known macromycetes with various medicinal properties. The aim of this study was to investigate the biological activities of Phellinus tuberculosus and Fuscoporia ferruginosa collected in Iran. The antimicrobial, antioxidant, and cytotoxic activities of the two species were examined, and their phenolic and polysaccharide contents were quantified. Compounds were characterized by HPLC-DAD chromatography and LC-ESI-MS/MS spectroscopy. According to our results, the antibacterial and antioxidant effects of P. tuberculosus extracts were stronger than F. ferruginosa. Also, the effect of hydroalcoholic extracts was higher than the aqueous extract. Gram-positive bacteria were more sensitive to all extracts, especially Streptococcus mutans with a MIC of 0.7 mg/mL and MBC of 6.25 mg/mL. HPLC-DAD analyses detected gallic acid, caffeic acid, and syringic acid in both fungi. The LC-ESI-MS/MS confirmed the detected compounds in HPLC-DAD and showed the presence of several phenolic compounds such as phellifuropyranone, phelligridin, and hispidin, besides others. This study showed that F. ferruginosa and P. tuberculosus are potent medicinal fungi with antibacterial and antioxidant properties, with no toxic effect on normal HDF cells, and possess various bioactive compounds including styrylpyrone-type phenols with well-known bioactivities.
- MeSH
- antibakteriální látky * chemie izolace a purifikace farmakologie MeSH
- antioxidancia * chemie izolace a purifikace farmakologie MeSH
- Basidiomycota * chemie metabolismus MeSH
- chromatografie kapalinová MeSH
- grampozitivní bakterie účinky léků MeSH
- Phellinus chemie MeSH
- tandemová hmotnostní spektrometrie MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Írán MeSH
Honey consumption is attributed to potentially advantageous effects on human health due to its antioxidant capacity as well as anti-inflammatory and antimicrobial activity, which are mainly related to phenolic compound content. Phenolic compounds are secondary metabolites of plants, and their content in honey is primarily affected by the botanical and geographical origin. In this study, a high-resolution mass spectrometry (HRMS) method was applied to determine the phenolic profile of various honey matrices and investigate authenticity markers. A fruitful sample set was collected, including honey from 10 different botanical sources (n = 51) originating from Greece and Poland. Generic liquid-liquid extraction using ethyl acetate as the extractant was used to apply targeted and non-targeted workflows simultaneously. The method was fully validated according to the Eurachem guidelines, and it demonstrated high accuracy, precision, and sensitivity resulting in the detection of 11 target analytes in the samples. Suspect screening identified 16 bioactive compounds in at least one sample, with abscisic acid isomers being the most abundant in arbutus honey. Importantly, 10 markers related to honey geographical origin were revealed through non-targeted screening and the application of advanced chemometric tools. In conclusion, authenticity markers and discrimination patterns were emerged using targeted and non-targeted workflows, indicating the impact of this study on food authenticity and metabolomic fields.
- MeSH
- antioxidancia analýza izolace a purifikace MeSH
- benzaldehydy analýza izolace a purifikace MeSH
- cinnamáty analýza izolace a purifikace MeSH
- flavonoidy analýza izolace a purifikace MeSH
- hmotnostní spektrometrie metody MeSH
- hydroxybenzoáty analýza izolace a purifikace MeSH
- lidé MeSH
- med analýza MeSH
- metabolom * MeSH
- metabolomika metody MeSH
- senzitivita a specificita MeSH
- správnost dat MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- validační studie MeSH
- Geografické názvy
- Polsko MeSH
- Řecko MeSH
The phenolic composition, as well as the antioxidant and antimicrobial activities of two poorly investigated Achillea species, Achillea lingulata Waldst. and the endemic Achillea abrotanoides Vis., were studied. To obtain a more detailed phytochemical profile, four solvents with different polarities were used for the preparation of the plant extracts whose phenolic composition was analyzed using UHPLC-MS/MS (ultra-high performance liquid chromatography-tandem mass spectrometry). The results indicate that both of the investigated Achillea species are very rich in both phenolic acids and flavonoids, but that their profiles differ significantly. Chloroform extracts from both species had the highest yields and were the most chemically versatile. The majority of the examined extracts showed antimicrobial activity, while ethanolic extracts from both species were potent against all tested microorganisms. Furthermore, the antioxidant activity of the extracts was evaluated. It was found that the ethanolic extracts possessed the strongest antioxidant activities, although these extracts did not contain the highest amounts of detected phenolic compounds. In addition, several representatives of phenolic compounds were also assayed for these biological activities. Results suggest that ethanol is a sufficient solvent for the isolation of biologically active compounds from both Achillea species. Moreover, it was shown that the flavonoids naringenin and morin are mainly responsible for these antimicrobial activities, while caffeic, salicylic, chlorogenic, p-coumaric, p-hydroxybenzoic, and rosmarinic acid are responsible for the antioxidant activities of the Achillea extracts.
- MeSH
- antiinfekční látky chemie izolace a purifikace farmakologie MeSH
- antioxidancia chemie izolace a purifikace farmakologie MeSH
- bifenylové sloučeniny antagonisté a inhibitory MeSH
- druhová specificita MeSH
- fenoly chemie izolace a purifikace farmakologie MeSH
- flavonoidy chemie izolace a purifikace farmakologie MeSH
- fytonutrienty chemie izolace a purifikace farmakologie MeSH
- hydroxybenzoáty chemie izolace a purifikace farmakologie MeSH
- mikrobiální testy citlivosti MeSH
- pikráty antagonisté a inhibitory MeSH
- řebříček chemie klasifikace MeSH
- rostlinné extrakty chemie MeSH
- rozpouštědla MeSH
- tandemová hmotnostní spektrometrie MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Publikační typ
- časopisecké články MeSH
Agrimonia eupatoria L. is an herb of the Rosaceae family, widely used in traditional (folk) medicine for its beneficial effects. Its water extracts (infusions and decoctions) are used in the treatment of airway and urinary system diseases, digestive tract diseases, and chronic wounds. Phytochemical analyses of Agrimonia eupatoria L. identified a variety of bioactive compounds including tannins, flavonoids, phenolic acids, triterpenoids and volatile oils possessing antioxidant, immunomodulatory and antimicrobial activities. The authors review the available literature sources examining and discussing the therapeutic and pharmacological effects of Agrimonia eupatoria L. at the molecular level in vitro and in vivo.
- MeSH
- Agrimonia * chemie MeSH
- antiinfekční látky izolace a purifikace terapeutické užití MeSH
- antioxidancia izolace a purifikace terapeutické užití MeSH
- fytonutrienty izolace a purifikace terapeutické užití MeSH
- imunologické faktory izolace a purifikace terapeutické užití MeSH
- lidé MeSH
- rostlinné extrakty izolace a purifikace terapeutické užití MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Detailed analysis of phenolic composition and antioxidant and antimicrobial activities of Verbascum glabratum subsp. bosnense (K. Malý) Murb., an endemic species of southeastern Dinaric Alps was performed for the first time. The phenolic composition measured via UHPLC-MS/MS of four extract with different polarity suggested this plant species is very rich in both phenolic acids and flavonoids. Ethanol extract was chemically the most versatile containing 12 compounds with quercitrin and rosmarinic acid as the majors, while water extracts were rich in 4-hydroxybenzoic acid, salicylic acid, morin, and apigenin. All extracts showed high antioxidant potential measured spectrophotometrically with IC50 values ranging 0.139 - 0.021 mg/mL. Antimicrobial testing using agar diffusion test showed that ethanol extract was the most potent against all tested organisms. Also, these activities are correlated with the content of phenolic compounds, which suggest they are active ingredients of the extracts.
- MeSH
- antiinfekční látky chemie izolace a purifikace MeSH
- antioxidancia chemie izolace a purifikace MeSH
- apigenin analýza MeSH
- fenoly chemie MeSH
- flavonoidy analýza MeSH
- quercetin analogy a deriváty analýza MeSH
- rostlinné extrakty chemie MeSH
- tandemová hmotnostní spektrometrie MeSH
- Verbascum chemie MeSH
- Publikační typ
- časopisecké články MeSH
Extensive phytochemical analysis of the CHCl3-soluble part of an ethanolic extract of branches and twigs of Broussonetia papyrifera led to the isolation of fourteen compounds, including a novel 5,11-dioxabenzo[b]fluoren-10-one derivative named broussofluorenone C (12). The isolated compounds 1-14 were characterized based on their NMR and HRMS data, and examined for their anti-inflammatory activities in LPS-stimulated THP-1 cells as well as for their cellular antioxidant effects. Compounds 7-10 and 12 showed inhibitory effects on NF-κB/AP-1 activation and compounds 7-9 were subsequently confirmed to suppress the secretion of both IL-1β and TNF-α in LPS-stimulated THP-1 cells more significantly than the prednisone used as a positive control. In the CAA assay, compound 10 exhibited the greatest antioxidant effect, greater than that of the quercetin used as a positive control. The results show possible beneficial effects and utilization of B. papyrifera wood in the treatment of inflammatory diseases as well as oxidative stress.
- MeSH
- antiflogistika chemie izolace a purifikace farmakologie MeSH
- antioxidancia chemie izolace a purifikace farmakologie MeSH
- Broussonetia chemie MeSH
- interleukin-1beta antagonisté a inhibitory biosyntéza MeSH
- kultivované buňky MeSH
- lidé MeSH
- lipopolysacharidy antagonisté a inhibitory farmakologie MeSH
- molekulární struktura MeSH
- NF-kappa B analýza antagonisté a inhibitory biosyntéza MeSH
- oxidační stres účinky léků MeSH
- THP-1 buňky MeSH
- TNF-alfa antagonisté a inhibitory biosyntéza MeSH
- transkripční faktor AP-1 analýza antagonisté a inhibitory biosyntéza MeSH
- viabilita buněk účinky léků MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- vztahy mezi strukturou a aktivitou MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Paeonia species have been valued for their ethnomedicinal uses in various countries and received much interest among the scientific community for their therapeutic properties, including anti-microbial, anti-inflammatory, anti-cancer, nephroprotective and hepatoprotective effects. The multiple phytotherapeutical applications of Paeonia species inspired us to establish the phytochemical fingerprint and to evaluate the biological properties of ethyl acetate, methanol, and aqueous extracts from the roots and aerial parts of two Paeonia species (P. arietina G. Anderson and P. kesrounansis Thiébaut). Phytoconstituents of P. arietina and P. kesrounansis extracts were analyzed using 1D and 2D NMR and LC-DAD-ESI-MS. The total content of phenolics (TPC) and flavonoids (TFC) in the extracts was also evaluated. The antioxidant activity was profiled using DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, and metal chelation assays. Enzyme inhibitory properties were evaluated against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, α-amylase, and α-glucosidase. Phytochemical analysis of P. arietina and P. kesrounansis extracts showed the presence of galloyl esters of sugars, galloyl monoterpenes, and glycosylated flavonoids. The three solvent extracts presented different behavior in the bioassays. The highest antioxidant activity, tyrosinase and AChE inhibition were observed for the methanolic extract of the aerial parts of P. kesrounansis. In addition, the ethyl acetate extracts of the aerial parts of both plants were the most effective inhibitors of α-amylase. The highest BChE inhibition was observed for root methanolic extract of P. kesrounansis while the root ethyl acetate extract of P. arietina exerted the strongest inhibition of α-glucosidase. Methanol extract of P. kesrounansis aerial parts presented the highest TPC, while TFC was greatest in the corresponding extract of P. arietina. Our findings can be considered as a starting point for future studies to further validate the effectiveness and safety profiles of these plants in folk medicine.
- MeSH
- antioxidancia chemie izolace a purifikace farmakologie MeSH
- chromatografie kapalinová metody MeSH
- fenoly analýza izolace a purifikace MeSH
- flavonoidy analýza izolace a purifikace MeSH
- hmotnostní spektrometrie s elektrosprejovou ionizací metody MeSH
- inhibitory enzymů chemie izolace a purifikace farmakologie MeSH
- kořeny rostlin MeSH
- nadzemní části rostlin MeSH
- Paeonia chemie MeSH
- rostlinné extrakty chemie farmakologie MeSH
- rozpouštědla chemie MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
Silybum marianum (L.) is a medicinal plant traditionally used in treatment of liver disorders. In last decades, silymarin (SM), a standardized extract from S. marianum seeds has been studied for its dermatological application, namely for UVB-protective properties. However, information on SM and its polyphenols effect on activity of enzymes participating in the (photo)aging process is limited. Therefore, evaluation of SM and its flavonolignans potential to inhibit collagenase, elastase, and hyaluronidase in tube tests was the goal of this study. The antioxidant and UV screening properties of SM and its flavonolignans silybin, isosilybin, silydianin, silychristin and 2,3-dehydrosilybin (DHSB) were also evaluated by a DPPH assay and spectrophotometrical measurement. DHSB showed the highest ability to scavenge DPPH radical and also revealed the highest UVA protection factor (PF-UVA) that corresponds with its absorption spectrum. SM and studied flavonolignans were found to exhibit anti-collagenase and anti-elastase activity. The most potent flavonolignan was DHSB. None of studied flavonolignans or SM showed anti-hyaluronidase activity. Our results suggest that SM and its flavonolignans may be useful agents for skin protection against the harmful effects of full-spectrum solar radiation including slowing down skin (photo)aging.
- MeSH
- antioxidancia chemie izolace a purifikace MeSH
- flavonolignany chemie izolace a purifikace MeSH
- kůže účinky léků patologie účinky záření MeSH
- lidé MeSH
- ostropestřec mariánský chemie MeSH
- rostlinné extrakty chemie MeSH
- semena rostlinná chemie MeSH
- silymarin chemie izolace a purifikace MeSH
- ultrafialové záření škodlivé účinky MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- antioxidancia * izolace a purifikace metabolismus normy MeSH
- bilirubin * izolace a purifikace krev metabolismus MeSH
- cholestáza * etiologie klasifikace metabolismus MeSH
- komorbidita MeSH
- kyselina cholová chemie metabolismus MeSH
- lidé MeSH
- metaanalýza jako téma MeSH
- receptory cytoplazmatické a nukleární metabolismus MeSH
- statistika jako téma MeSH
- žluč metabolismus MeSH
- žlučové kyseliny a soli chemie metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- přehledy MeSH
