E, PCR efficiency
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... and limitations of DNA cloning 61 -- 3.2 AMPLIFYING DNA USING THE -- POLYMERASE CHAIN REACTION -- (PCR ... ... ) 62 -- Basics of the polymerase chain reaction (PCR) 62 -- Quantitative PCR and real-time PCR.63 -- ... ... in the correspondence between phenotypes and the underlying genes and mutations -- 122 -- 124 -- • e ... ... advantages of ex vivo gene therapy 334 -- Viral delivery of therapeutic gene constructs: relatively high efficiency ... ... PATHOGENIC STRUCTURAL -- VARIATION 423 -- Screening for aneuploidies using quantitative fluorescence -- PCR ...
2nd ed. 534 s. : il.
"Genetics and Genomics in Medicine is a new textbook written for undergraduate and graduate students, as well as medical researchers, which explains the science behind the uses of genetics and genomics in medicine today. It is not just about rare inherited and chromosomal disorders, but how genetics affects the whole spectrum of human health and disease. DNA technologies are explained, with emphasis on the modern techniques that have revolutionized the use of genetic information in medicine and are indicating the role of genetics in common complex diseases. The detailed, integrative coverage of genetic approaches to treatment and prevention includes pharmacogenomics and the prospects for personalized medicine. Cancers are essentially genetic diseases and are given a dedicated chapter that includes new insights from cancer genome sequencing. Clinical disorders are covered throughout and there are extensive end-of-chapter questions and problems"--Provided by publisher.
... xix -- Contents -- OöCT-r 1 e>acx=r 2 ??????? ... ... -- OiaosrS -- OHDB-e OlHLJfcf 7 -- ¦tacer 10 -- INTRODUCTION TO THE CELL 1 -- Cells and Genomes 1 -- ... ... Exists Among Prokaryotic Cells ~ ‘e Has Three Primary Branches: Bacteria, Archaea, E -eryotes feres ... ... All Three Primary of the Tree of Life -e.eal the Functions of Genes 5cogv Began with a Spotlight on E. ... ... Cell Division -- 5 Specialized Nondividing State e 3i-Cdk and G-|/S-Cdk Activities E : : - s Cell Division ...
Sixth edition xxxiv, 1430 stran v různém stránkování : ilustrace (převážně barevné) ; 29 cm
- Konspekt
- Biochemie. Molekulární biologie. Biofyzika
- NLK Obory
- molekulární biologie, molekulární medicína
- NLK Publikační typ
- učebnice vysokých škol
... Scriven and Caroline Mackie Ogilvie -- Single cell PCR for PGD : methods, strategies, and limitations ... ... / Karen Sermon and Martine De Rycke -- Real-time quantitative PCR measurement of male fetal DNA in maternal ... ... [et al.] -- Cell-free fetal DNA plasma extraction and real-time PCR quantification / Jill L. ... ... from single cells / Kenneth E. ... ... Wangh -- Efficient isothermal amplification of the entire genome from single cells / Karen V. ...
Methods in molecular medicine, ISSN 1543-1894 132
xi, 182 s. : il., tab.
... Nested PCR 35 -- 3.4. Multiplex PCR 36 -- 3.5. Differential PCR 36 -- 3.6. Competitive PCR -- 3.7. ... ... Additives influencing the efficiency of PCR 55 -- 4.3.1. Dimethyl sulfoxide (DMSO) 55 -- 4.3.2. ... ... Evaluation of the effect of individual additives 58 -- Method: Improving the efficiency of PCR amplification ... ... Reverse transcription/PCR (RT-PCR) -- 9.1. Setting up an RT-PCR -- 9.2. ... ... Physical features of thermocyclers and of their influence on the efficiency of PCR amplification 259 ...
271 s. : il.
- MeSH
- klinické laboratorní techniky MeSH
- molekulární biologie MeSH
- polymerázová řetězová reakce MeSH
- Publikační typ
- monografie MeSH
- Konspekt
- Biologické vědy
- NLK Obory
- biologie
