Our basic cell biology research was aimed at investigating the effect on eukaryotic cells of the sudden loss of the F-actin cytoskeleton. Cells treated with latrunculin A (LA) in yeast extract peptone dextrose (YEPD) medium were examined using phase-contrast and fluorescent microscopy, freeze-substitution, transmission and scanning electron microscopy, counted using a Bürker chamber and their absorbance measured. The cells responded to the presence of LA, an F-actin inhibitor, with the disappearance of actin patches, actin cables and actin rings. This resulted in the formation of larger spherical cells with irregular morphology in the cell walls and ultrastructural disorder of the cell organelles and secretory vesicles. Instead of buds, LA-inhibited cells formed only 'table-mountain-like' wide flattened swellings without apical growth with a thinner glucan cell-wall layer containing β-1,3-glucan microfibrils. The LA-inhibited cells lysed. Actin cables and patches were required for bud formation and bud growth. In addition, actin patches were required for the formation of β-1,3-glucan microfibrils in the bud cell wall. LA has fungistatic, fungicidal and fungilytic effects on the budding yeast Saccharomyces cerevisiae.
- MeSH
- aktiny antagonisté a inhibitory MeSH
- antifungální látky farmakologie MeSH
- bicyklické sloučeniny heterocyklické farmakologie MeSH
- mikrobiální viabilita účinky léků MeSH
- mikroskopie MeSH
- počet mikrobiálních kolonií MeSH
- Saccharomyces cerevisiae cytologie účinky léků fyziologie MeSH
- Saccharomycetales cytologie účinky léků fyziologie MeSH
- thiazolidiny farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- aktiny MeSH
- antifungální látky MeSH
- bicyklické sloučeniny heterocyklické MeSH
- latrunculin A MeSH Prohlížeč
- thiazolidiny MeSH
