PURPOSE: To evaluate the clinical results of Epi-LASIK, a new surface ablation surgical technique for the treatment of low and middle myopia. METHODS: Twenty eyes of 10 patients had Epi-LASIK for the correction of low and middle myopia. Mean preoperative spherical equivalent of the refractive error was 3.64 diopters (D) +/- 1.9 D, and the best spectacle-corrected visual acuity was from 1.0 to 0.8 in 100 % of eyes. All epithelial separations were performed with the Krumiech-Barraquer microkeratome (Lasitome Gebauer). In the postoperative period the patients filled out a questionnaire grading pain score of the treated eyes. RESULTS: The mean uncorrected visual acuity at 1 month after the operation was from 1.0 to 0.8 in 90 % of eyes. After 12 months, the mean spherical equivalent of the refractive error of the treated eyes was -0.262 +/- 0.15 D (from -0.25 to +0.25 D). One hundred percent of eyes had clear corneas at 6 months after the treatment. After 24 months, the mean spherical equivalent of the refractive error was -0.258 I 0.14 D, no "haze" was observed. CONCLUSIONS: The first clinical results suggest that Epi-LASIK is a safe, less painful and efficient method for the correction of low and middle myopia. We suppose, further studies will establish this method as a very good alternative surface ablation procedure.
This study uses immunofluorescent analysis to find out in histologic corneal sections a possible rejection reaction in anterior lens capsule allotransplantation for chronic corneal ulcers. The experiment included control group of rabbit corneas with repeat injury to epithelium and anterior stroma as well as surgically treated group. Fluorescein-conjugated human antibodies to IgA, IgM, IgG, complement and fibrinogen were used in the study. For detection we used method of direct immunofluorescence. Positive was only examination on fibrinogen in sections of treated group eyes 2 days after surgery. Last examination after 3 months failed to detect positivity in any of the tested markers. We preliminarily conclude that anterior lens capsule allotransplantation for chronic corneal ulcers does not result in rejection reaction and that components of humoral immunity do not participate in immune reaction.
- MeSH
- chronická nemoc MeSH
- fluorescenční protilátková technika přímá MeSH
- homologní transplantace MeSH
- imunoglobuliny analýza MeSH
- komplement C3 analýza MeSH
- králíci MeSH
- pouzdro oční čočky imunologie transplantace MeSH
- vřed rohovky chirurgie MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- imunoglobuliny MeSH
- komplement C3 MeSH
The purpose of this study was to identify limbal stem cells in the culture of human donor cornea. After standard cultivation procedure we used monoclonal antibody against alpha-enolase for identification of stem cells. This antibody serves as a probe for the identification of biochemical marker of stem cells. Using immunofluorescent microscopy we identified in specimens alpha-enolase positive cells which in vitro but not in situ showed also some morphologic differences from other cellular elements of the culture.
- MeSH
- fluorescenční protilátková technika * MeSH
- fosfopyruváthydratasa analýza imunologie MeSH
- kmenové buňky chemie cytologie MeSH
- kultivované buňky MeSH
- lidé MeSH
- limbus corneae cytologie MeSH
- monoklonální protilátky MeSH
- transplantace rohovky * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- fosfopyruváthydratasa MeSH
- monoklonální protilátky MeSH
The aim of this experimental study was to evaluate the role of limbal stem cells on corneal wound healing. The experimental objects were New Zealand white rabbits. Damage to the cornea was caused by 2.5 N NaOH solution for 50 sec. and was subsequently washed out by saline solution. The rabbits were euthanased 24 hours after alkaly burn and the tissue was fixated in 10% formalin. The samples were embedded in paraffin and stained with haematoxilin and eosin. The specimen showed focal inflammatory reaction with the presence of macrophages an round--nucleated cells in the limbal area.
- MeSH
- hydroxid sodný MeSH
- králíci MeSH
- limbus corneae patologie MeSH
- popálení oka patologie MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- hydroxid sodný MeSH
The author presents the results of ND: YAG trabeculotomy on 10 eyes with open-angle glaucoma. The surgery has been carried out as last treatment before trabeculectomy. The mean intraocular pressure (IOP) before laser surgery was 35 +/- 5.2 mm Hg. The IOP decrease below 22.0 mm Hg has been achieved in 50% of eyes. The mean follow-up of the patients was 1 year. Complications included transient postoperative IOP elevation (six eyes) and angle bleeding (three eyes).
- MeSH
- dospělí MeSH
- glaukom chirurgie MeSH
- laserová terapie * MeSH
- lidé středního věku MeSH
- lidé MeSH
- senioři MeSH
- trabekulektomie * MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
The results of diode laser trabeculoplasty carried out on 14 eyes of primary open-angle glaucoma are demonstrated. Powers of 0.8-1.0 W were used, with spot 75 um and a pulse of 0.1 second and a mean ocular hypotensive effect of 6.82 +/- 2.94 mmHg (27.9%) was observed at 6 months after treatment. The 78.6% compensation was achieved. The results confirm that diode laser trabeculoplasty is an effective mode of treatment for eyes with primary open-angle glaucoma.
- MeSH
- glaukom s otevřeným úhlem chirurgie MeSH
- laserová terapie * MeSH
- lidé středního věku MeSH
- lidé MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- trabekulektomie * MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
In a group of 57 eyes in 51 patients the authors analyze seven years' experience with the use of an argon laser in the prophylaxis of detachment of the retina. The advantages of the argon laser were most marked in traumatic ruptures of the retina and in peripheral vitreoretinal degenerations. In eyes with not closed ruptures after episcleral filling argon laser coagulation proved successful in 77%.
- MeSH
- dítě MeSH
- dospělí MeSH
- laserová koagulace * MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- odchlípení sítnice etiologie prevence a kontrola MeSH
- retina zranění MeSH
- senioři MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
The authors discuss the advantages of ultrasonograms type b when assessing pathological intrabulbar conditions, not only when the ocular media are turbid. They emphasize that the examination is unpretentious as regards time and funds and is well tolerated by children and neonates.
- MeSH
- cizí těleso v oku diagnostické zobrazování MeSH
- lidé MeSH
- nádory oka diagnostické zobrazování MeSH
- oči diagnostické zobrazování MeSH
- ultrasonografie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
In group of 202 eyes in 125 patients with primary open-angle glaucoma with an observation period from 12 to 66 months (32.6 z 4.8 months) the effectiveness of ALT was analyzed in relation to the time factor. The reduction of the intraocular pressure was statistically significant and treatment was successful when the intraocular pressure was reduced beneath 2.66 kPa (20 mm Hg) in 64.4%. Changes of the visual field developed in 23.3% and the efflux rate increased by 28.6%. The authors compared also the effectiveness of two methods, 180 degrees ALT 1.0 W and 180 degrees ALT 0.5 W which did not differ significantly.
- MeSH
- glaukom s otevřeným úhlem chirurgie MeSH
- laserová terapie * MeSH
- lidé MeSH
- trabekulektomie * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
The authors submit information on the contemporary state of epikeratophakia in the world. They mention its advantages, surgical technique and initial experimental results. They pay also attention to problems associated with its introduction into clinical practice in this country.
- MeSH
- lidé MeSH
- metody MeSH
- refrakční chirurgické výkony * MeSH
- transplantace rohovky * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
