The luteinizing hormone receptor (LHCGR) has a little studied polymorphic 6 bp insertion (rs4539842/insLQ). This study has evaluated the insLQ polymorphism in relation to potential associations with hormonal characteristics of human small antral follicles (hSAFs). In total, 310 hSAFs were collected from 86 women undergoing fertility preservation. Analysis included hormonal profile of 297 follicular fluid (FF) samples and 148 corresponding granulosa cells samples were evaluated by qPCR for selected genes. Significantly reduced and non-detectable mRNA levels of anti-Müllerian hormone receptor II (AMHR2) and LHCGR, respectively, were observed for insLQ/insLQ compared to -/insLQ and the -/- genotypes. Moreover, LHCGR and CYP19a1 together with oestradiol and inhibin-B were significantly increased in -/insLQ compared to the -/- genotype. The homozygous insLQ genotype showed strong significant associations to GC specific genes LHCGR and CYP19a1, which may translate into significant changes in FF hormone profiles and an altered LH signaling.
- Klíčová slova
- Follicle fluid, Human small antral follicles, LHR, rs4539842,
- MeSH
- dospělí MeSH
- folikulární buňky metabolismus MeSH
- folikulární tekutina metabolismus MeSH
- genotyp MeSH
- hormony metabolismus MeSH
- inzerční mutageneze * MeSH
- lidé MeSH
- messenger RNA genetika metabolismus MeSH
- mladiství MeSH
- mladý dospělý MeSH
- ovariální folikul metabolismus MeSH
- polymorfismus genetický * MeSH
- receptory LH genetika MeSH
- regulace genové exprese * MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- hormony MeSH
- messenger RNA MeSH
- receptory LH MeSH
