The role of nonneuronal cells in the resolution of cerebral ischemia remains to be fully understood. To decode key molecular and cellular processes that occur after ischemia, we performed spatial and single-cell transcriptomic profiling of the male mouse brain during the first week of injury. Cortical gene expression was severely disrupted, defined by inflammation and cell death in the lesion core, and glial scar formation orchestrated by multiple cell types on the periphery. The glial scar was identified as a zone with intense cell-cell communication, with prominent ApoE-Trem2 signaling pathway modulating microglial activation. For each of the three major glial populations, an inflammatory-responsive state, resembling the reactive states observed in neurodegenerative contexts, was observed. The recovered spectrum of ischemia-induced oligodendrocyte states supports the emerging hypothesis that oligodendrocytes actively respond to and modulate the neuroinflammatory stimulus. The findings are further supported by analysis of other spatial transcriptomic datasets from different mouse models of ischemic brain injury. Collectively, we present a landmark transcriptomic dataset accompanied by interactive visualization that provides a comprehensive view of spatiotemporal organization of processes in the postischemic mouse brain.

• Klíčová slova
• glia, ischemic stroke, neuroinflammation, single-cell transcriptomics, spatial transcriptomics,
• MeSH
• ischemie mozku * genetika   metabolismus   patologie   MeSH
• membránové glykoproteiny metabolismus   genetika   MeSH
• mikroglie metabolismus   MeSH
• modely nemocí na zvířatech * MeSH
• mozek metabolismus   patologie   MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• neuroglie * metabolismus   MeSH
• oligodendroglie metabolismus   MeSH
• receptory imunologické MeSH
• signální transdukce MeSH
• stanovení celkové genové exprese MeSH
• transkriptom * MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• membránové glykoproteiny MeSH
• receptory imunologické MeSH
• Trem2 protein, mouse MeSH Prohlížeč