Hybrid biocomposite scaffolds (HBS) that serve as a carrier for cell proliferation and differentiation are increasingly used for tissue regeneration. 3D hybrid scaffold based on collagen-grafted-chitosan-glucan fiber (CO-g-CGF-HBS) was prepared by freeze-drying technique. The swelling percentage, hydrolytic stability, and modulus of elasticity of HBS were enhanced after the chemical modification of CO with CGF. Pore size and porosity of HBS were decreased with an increased CGF ratio. HBS exhibits a higher reduction rate against different types of bacteria compared with a control sample. Thus, chemical modification of CO with different ratios of CGF significantly improved the physicochemical, antibacterial properties of HBS.
- Klíčová slova
- Antibacterial and mechanical properties, Chitosan-glucan fiber, Collagen, Hybrid scaffolds,
- MeSH
- biokompatibilní materiály chemie MeSH
- chitosan * chemie MeSH
- glukany MeSH
- kolagen chemie MeSH
- poréznost MeSH
- proliferace buněk MeSH
- tkáňové inženýrství metody MeSH
- tkáňové podpůrné struktury chemie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- biokompatibilní materiály MeSH
- chitosan * MeSH
- glukany MeSH
- kolagen MeSH
Collagen (CO)/chitosan-glucan complex (CSGC) hollow fibers encapsulated aloe vera (AV) dressing scaffold (CO/CSGC@AV) were fabricated for the first time by the freeze-dried process. Extraction process, morphology, mechanical properties, pore size, porosity, swelling ability, and degradation behavior of composites scaffold were investigated. CSGC hollow fibers were extracted from mycelium of Schizophyllum commune CSGC hollow fiber exhibited inner diameter of (600 ± 250 nm) and outer fiber diameter of (2.5 ± 0.5 μm). The results of swelling and hydrolytic degradation studies demonstrated that the physicochemical of CO/CSGC@AV was significantly enhanced by CSGC in a concentration-dependent manner. The mechanical property of the CO/CSGC@AV was improved after encapsulated AV into CSGC hollow fibers compared with native CO. The pore size and porosity of the CO/CSGC@AV were slightly decreased in the presence of AV. All these results suggested that the new dressing scaffold has a potential for clinical skin regeneration, particularly for infected chronic wounds and ulcers.
- Klíčová slova
- Aloe vera, Chitosan-glucan, Collagen, Degradation, Hollow fiber, Release properties, Wound dressing,
- MeSH
- Aloe metabolismus MeSH
- chitosan * chemie izolace a purifikace MeSH
- glukany * chemie izolace a purifikace MeSH
- kolagen * chemie izolace a purifikace MeSH
- lyofilizace MeSH
- obvazy * MeSH
- pevnost v tahu MeSH
- poréznost MeSH
- rostlinné extrakty chemie MeSH
- Schizophyllum metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- chitosan * MeSH
- glukany * MeSH
- kolagen * MeSH
- rostlinné extrakty MeSH
Novel nanofibers mats were fabricated by electrospinning of polyvinyl alcohol/hyaluronan (PVA/HA) solutions in the presence of silver nanoparticles (AgNPs). The AgNPs were synthesized by in-situ chemical reduction of silver ions (Ag+) using HA as a reducing and stabilizing agent. Narrow size distribution and spherical shape of AgNPs were achieved by optimizing the initial silver nitrate concentration (0.01 to 1 M) and reaction time (10-60 min). HA-AgNPs nanocomposite and PVA/HA-AgNPs nanofibrous mats were fabricated by electrospinning technique from aqueous solution containing a different mass ratio of PVA and HA-AgNPs and characterized by UV/Vis spectroscopy, SEM, TEM, DLS, XRD, TGA, and ATR-FTIR. Mechanical and rheological properties were also investigated and discussed. The novel nanofibrous mats show great potential in skin regeneration and drug carrier applications.
- Klíčová slova
- Electrospinning, Hyaluronan, Nanocomposite, Nanofiber, in-situ AgNPs,
- MeSH
- dusičnan stříbrný chemie MeSH
- elektrochemie MeSH
- ionty MeSH
- kovové nanočástice chemie MeSH
- kyselina hyaluronová chemie MeSH
- nanokompozity chemie MeSH
- nanovlákna MeSH
- nosiče léků MeSH
- obvazy MeSH
- polyvinylalkohol chemie MeSH
- povrchové vlastnosti MeSH
- reologie MeSH
- rozpouštědla MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- stříbro chemie MeSH
- technologie zelené chemie * MeSH
- velikost částic MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- dusičnan stříbrný MeSH
- ionty MeSH
- kyselina hyaluronová MeSH
- nosiče léků MeSH
- polyvinylalkohol MeSH
- rozpouštědla MeSH
- stříbro MeSH
In-situ formed hyaluronan/silver (HA/Ag) nanoparticles (NPs) were used to prepare composite fibers/fabrics for the first time. Different concentrations of silver nitrate (1, 2mg/100ml) were added at ambient temperature to sodium hyaluronate solution (40mg/ml), then the pH was increased to 8 by adding sodium hydroxide. The in-situ formed HA/Ag-NPs were used to prepare fibers/nonwoven fabrics by wet-dry-spinning technique (WDST). UV/vis spectroscopy, SEM, TEM, DLS, XPS, XRD and TGA were employed to characterize the structure and composition of the nanocomposite, surface morphology of fiber/fabrics, particle size of Ag-NPs, chemical interactions of Ag0 and HA functional groups, crystallinity and thermal stability of the wound dressing, respectively. The resultant HA/Ag-NPs1 and HA/Ag-NPs2 composite showed uniformly dispersed throughout HA fiber/fabrics (SEM), an excellent distribution of Ag-NPs with 25±2, nm size (TEM, DLS) and acceptable mechanical properties. The XRD analysis showed that the in-situ preparation of Ag-NPs increased the crystallinity of the resultant fabrics as well as the thermal stability. The antibacterial performance of medical HA/Ag-NPs fabrics was evaluated against gram negative bacteria E. coli K12, exhibiting significant bactericidal activity. The fibers did not show any cytotoxicity against human keratinocyte cell line (HaCaT). In-vivo animal tests indicated that the prepared wound dressing has strong healing efficacy (non-diabetics/diabetics rat model) compared to the plain HA fabrics and greatly accelerated the healing process. Based on our results, the new HA/Ag-NPs-2mg nonwoven wound dressing fabrics can be used in treating wounds and chronic ulcers as well as cell carrier in different biological research and tissue engineering.
- Klíčová slova
- Cytotoxicity, Hyaluronan, In-situ silver, Wound dressing,
- MeSH
- hojení ran účinky léků MeSH
- krysa rodu Rattus MeSH
- krystalizace MeSH
- kultivované buňky MeSH
- kyselina hyaluronová chemie terapeutické užití MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- nanovlákna chemie terapeutické užití ultrastruktura MeSH
- obvazy * MeSH
- stabilita léku MeSH
- stříbro chemie MeSH
- velikost částic MeSH
- vřed farmakoterapie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- lidé MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- kyselina hyaluronová MeSH
- stříbro MeSH
