The detection of cancer antigens is a major aim of cancer research in order to develop better patient management through early disease detection. Many cancers including prostate, lung, and ovarian secrete a protein disulfide isomerase protein named AGR2 that has been previously detected in urine and plasma using mass spectrometry. Here we determine whether a previously developed monoclonal antibody targeting AGR2 can be adapted from an indirect two-site ELISA format into a direct detector using solid-phase printed gold electrodes. The screen-printed gold electrode was surface functionalized with the anti-AGR2 specific monoclonal antibody. The interaction of the recombinant AGR2 protein and the anti-AGR2 monoclonal antibody functionalized electrode changed its electrochemical impedance spectra. Nyquist diagrams were obtained after incubation in an increasing concentration of purified AGR2 protein with a range of concentrations from 0.01 fg/mL to 10 fg/mL. In addition, detection of the AGR2 antigen can be achieved from cell lysates in medium or artificial buffer. These data highlight the utility of an AGR2-specific monoclonal antibody that can be functionalized onto a gold printed electrode for a one-step capture and quantitation of the target antigen. These platforms have the potential for supporting methodologies using more complex bodily fluids including plasma and urine for improved cancer diagnostics.
- Klíčová slova
- AGR2 protein, electrochemical impedance spectroscopy, screen-printed gold electrode, sensor,
- MeSH
- biosenzitivní techniky * MeSH
- elektrochemické techniky MeSH
- elektrody MeSH
- kovové nanočástice MeSH
- lidé MeSH
- limita detekce MeSH
- monoklonální protilátky MeSH
- mukoproteiny analýza MeSH
- nádory MeSH
- onkogenní proteiny analýza MeSH
- zlato MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- AGR2 protein, human MeSH Prohlížeč
- monoklonální protilátky MeSH
- mukoproteiny MeSH
- onkogenní proteiny MeSH
- zlato MeSH
