We report the genome sequence of the pathogenic yeast Candida parapsilosis strain SR23 (CBS 7157) used in a number of experimental studies. The nuclear genome assembly consists of eight chromosome-sized contigs with a total size of 13.04 Mbp (N50 2.09 Mbp) and a G+C content of 38.7%.
- Klíčová slova
- Candida, genome analysis, yeasts,
- Publikační typ
- časopisecké články MeSH
Ribosomes are ribonucleoprotein complexes highly conserved across all domains of life. The size differences of ribosomal RNAs (rRNAs) can be mainly attributed to variable regions termed expansion segments (ESs) protruding out from the ribosomal surface. The ESs were found to be involved in a range of processes including ribosome biogenesis and maturation, translation, and co-translational protein modification. Here, we analyze the rRNAs of the yeasts from the Magnusiomyces/Saprochaete clade belonging to the basal lineages of the subphylum Saccharomycotina. We find that these yeasts are missing more than 400 nt from the 25S rRNA and 150 nt from the 18S rRNAs when compared to their canonical counterparts in Saccharomyces cerevisiae. The missing regions mostly map to ESs, thus representing a shift toward a minimal rRNA structure. Despite the structural changes in rRNAs, we did not identify dramatic alterations in the ribosomal protein inventories. We also show that the size-reduced rRNAs are not limited to the species of the Magnusiomyces/Saprochaete clade, indicating that the shortening of ESs happened independently in several other lineages of the subphylum Saccharomycotina.
- Klíčová slova
- Magnusiomyces, expansion segments, ribosomal RNA, ribosome, yeast,
- MeSH
- fylogeneze MeSH
- molekulární evoluce MeSH
- ribozomální proteiny genetika MeSH
- ribozomy * metabolismus genetika MeSH
- RNA ribozomální 18S genetika MeSH
- RNA ribozomální * genetika MeSH
- Saccharomyces cerevisiae genetika MeSH
- Saccharomycetales genetika klasifikace metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- ribozomální proteiny MeSH
- RNA ribozomální 18S MeSH
- RNA ribozomální * MeSH
- RNA, ribosomal, 25S MeSH Prohlížeč
