Klebsiella pneumoniae infections have always been an important problem in public health, but today, the increasing resistance of these bacteria to antibiotics due to β-lactamases production has renewed interest in K. pneumoniae infections. The aim of the study was to present a case of a neurosurgical patient with multidrug-resistant K. pneumoniae ST11 infection after craniectomy. Four K. pneumoniae isolates from various clinical materials of the patient undergone identification and susceptibility testing with the Vitek2 system. Tests for β-lactamases production were performed according to EUCAST guidelines. Strains were analyzed for bla genes responsible for β-lactamase production (blaTEM, blaSHV, blaCTX-M, blaVIM, blaIMP, blaNDM, blaKPC, blaOXA-48) using PCR. Moreover, the genetic relatedness of these isolates was determined by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). All tested strain presented multidrug resistance. The highest susceptibility was observed for imipenem, meropenem, and ertapenem. The strain isolated from the nervous system was ESBL-positive with blaSHV-11, blaTEM-1, and blaCTX-M-15 genes. Additionally, the strain from urine was blaKPC-3-positive. Molecular typing revealed that all strains belonged to the same clone and identified two PFGE profiles. The analysis of MLST allelic profile showed that tested K. pneumoniae strains belonged to ST11. Identification of ST11 K. pneumoniae as etiological factor of infection unfavorably impacts on prognosis among neurosurgical patient after craniectomy.
- MeSH
- antibakteriální látky farmakologie terapeutické užití MeSH
- beta-laktamasy genetika MeSH
- fatální výsledek MeSH
- infekce bakteriemi rodu Klebsiella diagnostické zobrazování farmakoterapie mikrobiologie MeSH
- Klebsiella pneumoniae klasifikace účinky léků enzymologie MeSH
- kraniotomie škodlivé účinky MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- mladý dospělý MeSH
- mnohočetná bakteriální léková rezistence * MeSH
- multilokusová sekvenční typizace MeSH
- počítačová rentgenová tomografie MeSH
- pulzní gelová elektroforéza MeSH
- techniky typizace bakterií MeSH
- Check Tag
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- kazuistiky MeSH
- Názvy látek
- antibakteriální látky MeSH
- beta-laktamasy MeSH
Differentiation of the oxidase positive staphylococci, Staphylococcus sciuri, Staphylococcus lentus, Staphylococcus vitulinus and Staphylococcus fleurettii, based on tributyrin, urease, caseinase, gelatinase and DNase activity is described. These tests may be used for preliminary identification of oxidase positive isolates of staphylococci resulting in more accurate identification of these species.
- MeSH
- deoxyribonukleasy metabolismus MeSH
- druhová specificita MeSH
- lidé MeSH
- metaloendopeptidasy metabolismus MeSH
- mikrobiologie životního prostředí MeSH
- oxidoreduktasy metabolismus MeSH
- potravinářská mikrobiologie MeSH
- senzitivita a specificita MeSH
- stafylokokové infekce mikrobiologie MeSH
- Staphylococcus klasifikace enzymologie MeSH
- techniky typizace bakterií metody MeSH
- triglyceridy metabolismus MeSH
- ureasa metabolismus MeSH
- želatinasy metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Jugoslávie MeSH
- Názvy látek
- caseinase MeSH Prohlížeč
- deoxyribonukleasy MeSH
- metaloendopeptidasy MeSH
- oxidoreduktasy MeSH
- tributyrin MeSH Prohlížeč
- triglyceridy MeSH
- ureasa MeSH
- želatinasy MeSH
