Macrococci are usually found as commensals on the skin and mucosa of animals and have been isolated from mammal-derived fermented foods; however, they can also act as opportunistic pathogens. Here, we used whole-genome sequencing, comparative genomics, extensive biotyping, MALDI-TOF mass spectrometry, and chemotaxonomy to characterize Macrococcus sp. strains isolated from livestock and human-related specimens. Based on the results of polyphasic taxonomy, we propose the species Macrococcus psychrotolerans sp. nov. (type strain NRL/St 95/376T = CCM 8659T = DSM 111350T) belonging to the Macrococcus caseolyticus phylogenetic clade. It grows at 4°C, and the core genome of the isolates contains suspected genes contributing to low-temperature tolerance. Variable genetic elements include prophages, chromosomal islands, a composite staphylococcal cassette chromosome island, and many plasmids that affect the overall genome expansion and adaptation to specific ecological settings of the studied isolates. Large plasmids carrying the methicillin resistance gene mecB were identified in M. psychrotolerans sp. nov. strains and confirmed as self-transmissible to Staphylococcus aureus in vitro. In addition to plasmids with circular topology, a 150-kb-long linear plasmid with 14.1-kb-long inverted terminal repeats, harboring many IS elements and putative genes for a type IV secretion system was revealed. The described strains were isolated from human clinical material, food-producing animals, meat, and a wooden cheese board and have the potential to proliferate at refrigerator temperatures. Their presence in the food chain and human infections indicates that attention needs to be paid to this potential novel opportunistic pathogen.IMPORTANCEThe study offers insights into the phenotypic and genomic features of a novel species of the genus Macrococcus that occurs in livestock, food, and humans. The large number of diverse mobile genetic elements contributes to the adaptation of macrococci to various environments. The ability of the described microorganisms to grow at refrigerator temperatures, enabled by genes that are predicted to contribute to low-temperature tolerance, raises food safety concerns. Confirmed in vitro conjugative transfer of plasmid-borne mecB gene to S. aureus poses a significant risk of spread of broad β-lactam resistance. In addition, the intergeneric plasmid transfer to S. aureus is indicative of horizontal gene transfer events that may be more frequent than generally accepted. Determining a complete sequence and gene content of linear megaplasmid with exceptional topology for the Staphylococcaceae family suggests its possible role in shuttling adaptive traits through an exchange of genetic information.
- Klíčová slova
- Gram-positive cocci, cephalosporin resistance, cold temperature tolerance, conjugation, food safety, linear plasmid, methicillin resistance,
- MeSH
- antibakteriální látky * farmakologie MeSH
- bakteriální proteiny * genetika metabolismus MeSH
- beta-laktamová rezistence * genetika MeSH
- dobytek mikrobiologie MeSH
- Enterococcaceae * genetika izolace a purifikace účinky léků MeSH
- fylogeneze MeSH
- genom bakteriální MeSH
- lidé MeSH
- plazmidy MeSH
- přenos genů horizontální MeSH
- sekvenování celého genomu MeSH
- stafylokokové infekce mikrobiologie MeSH
- Staphylococcus aureus * genetika účinky léků MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky * MeSH
- bakteriální proteiny * MeSH
Mine aerosol poses a serious health threat due to its easy access to the human respiratory tract. Damage may be caused by the chemical composition of dust and the substances adsorbed on its surface, including microorganisms that potentially affect human health. Our proposed research aimed to isolate Staphylococcus aureus strains from coal mine bioaerosol and to assess its sensitivity towards selected antibiotics. Bioaerosol samples were collected in three underground hard coal mines located in Upper Silesia in southern Poland. Microbiological tests of the air samples were carried out according to standard microbiological techniques. All tested strains of Staphylococcus aureus were sensitive to oxacillin, which indicated the lack of methicillin-resistant isolates (MRSA) in the tested group. However, antibiotic resistance from macrolide and lincosamide groups was observed among certain strains. 10% of isolates were constitutive MLSB resistance, while 4% of strains were inductive MLSB resistance. Less than 1% of isolates were erythromycin-resistant and clindamycin-sensitive (MSB). Based on the Chi-square test, statistically significant differences were found in the frequency of MSB, MLSB inductive, and MLSB constitutive phenotypes. Almost 30% of the identified strains showed multi-antibiotic resistance. However, the Chi-square test did not reveal any statistically significant differences in the frequency of multidrug-resistant strains in the considered research areas. The analyses carried out constituted the first study related to the isolation and assessment of drug susceptibility of Staphylococcus aureus in the bioaerosol of hard coal mines. Identification of bioaerosol in underground coal mines is a key issue because, due to the presence of pathogens, it plays a significant role in limiting the spread of occupational diseases. For the health of miners, research into microbial communities benefits the promotion of microbiological control of mine air.
- Klíčová slova
- Bioaerosol, Drug sensitivity, Mining excavations, Respirable dust, Staphylococci,
- MeSH
- antibakteriální látky * farmakologie MeSH
- bakteriální léková rezistence * MeSH
- lidé MeSH
- methicilin rezistentní Staphylococcus aureus izolace a purifikace účinky léků MeSH
- mikrobiální testy citlivosti MeSH
- mikrobiologie vzduchu * MeSH
- mnohočetná bakteriální léková rezistence MeSH
- Staphylococcus aureus * účinky léků izolace a purifikace MeSH
- těžba uhlí * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Polsko MeSH
- Názvy látek
- antibakteriální látky * MeSH
BACKGROUND: Antimicrobial lock therapy is recommended for preventing and treating catheter-related bloodstream infections, but different solutions have uncertain efficacy. METHODS: Two locks, 1.35% taurolidine and 4% ethylenediaminetetraacetic acid (EDTA), were tested on Staphylococcus epidermidis, Staphylococcus aureus, methicillin-resistant S. aureus, Pseudomonas aeruginosa, multidrug-resistant P. aeruginosa, vancomycin-resistant Enterococcus faecium, Klebsiella oxytoca (carbapenemase producing), K. pneumoniae (extended-spectrum β-lactamase producing), Candida albicans, and Candida glabrata. Broviac catheter segments were incubated with these organisms and then exposed to various lock solutions. Colony-forming units (CFUs) were counted after 2, 4, and 24 h of incubation. RESULTS: Taurolidine showed a significant decrease in CFUs after 2 h in S. aureus, S. epidermidis, methicillin-resistant S. aureus, vancomycin-resistant E. faecium, P. aeruginosa (both sensitive and multidrug-resistant strains), K. oxytoca, C. albicans, and C. glabrata. After 4 h, significant reductions were noted in S. aureus, S. epidermidis, methicillin-resistant S. aureus, P. aeruginosa, multidrug-resistant P. aeruginosa, K. pneumoniae, K. oxytoca, and C. albicans. Taurolidine was also effective after 24 h, especially against methicillin-resistant S. aureus and multidrug-resistant P. aeruginosa. Four percent EDTA acid showed a significant reduction in CFUs after 2 h in S. aureus, vancomycin-resistant E. faecium, P. aeruginosa, K. oxytoca, C. albicans, and C. glabrata. After 4 h, reductions occurred in P. aeruginosa, multidrug-resistant P. aeruginosa, K. oxytoca, and C. albicans and after 24 h in methicillin-resistant S. aureus, P. aeruginosa, and K. oxytoca. CONCLUSION: Taurolidine is more effective than 4% EDTA acid in eradicating Gram-positive and Gram-negative microorganisms and fungi.
- Klíčová slova
- antimicrobial lock, catheter‐related blood stream infection, ethylenediaminetetraacetic acid, home parenteral nutrition, taurolidine, venous catheter,
- MeSH
- antiinfekční látky * farmakologie MeSH
- Candida albicans účinky léků MeSH
- EDTA * farmakologie MeSH
- katétrové infekce * prevence a kontrola mikrobiologie MeSH
- lidé MeSH
- methicilin rezistentní Staphylococcus aureus účinky léků MeSH
- mikrobiální testy citlivosti MeSH
- počet mikrobiálních kolonií MeSH
- Pseudomonas aeruginosa účinky léků MeSH
- Staphylococcus aureus účinky léků MeSH
- Staphylococcus epidermidis účinky léků MeSH
- taurin * analogy a deriváty farmakologie MeSH
- thiadiaziny * farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Názvy látek
- antiinfekční látky * MeSH
- EDTA * MeSH
- taurin * MeSH
- taurolidine MeSH Prohlížeč
- thiadiaziny * MeSH
Selenium nanoparticles (SeNPs) have recently attracted attention for their antimicrobial and anticancer activities. Nevertheless, their use remains limited due to stability issues. The objective of this study is to investigate the impact of different reaction conditions (including the reducing and stabilizing agents, as well as reaction temperature) on the water dispersion characteristics, stability, and biological activity of SeNPs. The particle characteristics were controlled using sodium borohydride as a strong reducing agent and ascorbic acid as a mild agent. The impact of different stabilizers, namely sodium oleate, quercetin, gelatine, poly(ethyleneimine), and poly(diallyldimethyl-ammonium chloride), was investigated on both particle stability and biological activity. Several destabilizing processes occurred, one of which was continuous reduction to the final Se(-II) oxidation state, which was observed in both synthetic approaches, with using sodium borohydride or ascorbic acid as reducing agents. Non-stabilized SeNP dispersions were stable for a maximum of two weeks, while most stabilized SeNP dispersions remained stable for at least two months, and some remained stable for as long as six months. The antibacterial activity had strong effects, particularly against Gram-positive bacteria, and simultaneously low cytotoxicity against mammalian cells. SeNPs exhibited significant antibacterial efficacy, particularly against Staphylococcus aureus, including methicillin-resistant Staphylococcus aureus strains, even at concentrations as low as 1 mg L-1. SeNPs synthesized utilizing sodium borohydride demonstrate minimal cytotoxicity (EC50 > 100 mg L-1). Interestingly, SeNPs reduced by ascorbic acid demonstrated higher cytotoxicity (EC50 6.8 mg L-1) against the NIH/3T3 cell line. This effect is likely due to the combined cytotoxic effect of SeNPs and ascorbic acid acting as a pro-oxidant at high concentrations.
- MeSH
- antibakteriální látky * farmakologie chemie chemická syntéza MeSH
- borohydridy * chemie MeSH
- buňky NIH 3T3 MeSH
- kovové nanočástice chemie MeSH
- kyselina askorbová * chemie farmakologie MeSH
- mikrobiální testy citlivosti MeSH
- myši MeSH
- nanočástice chemie MeSH
- oxidace-redukce MeSH
- redukční činidla chemie farmakologie MeSH
- selen * chemie farmakologie MeSH
- Staphylococcus aureus účinky léků MeSH
- velikost částic MeSH
- viabilita buněk účinky léků MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky * MeSH
- borohydridy * MeSH
- kyselina askorbová * MeSH
- redukční činidla MeSH
- selen * MeSH
- sodium borohydride MeSH Prohlížeč
Infections caused by antibiotic-drug-resistant microorganisms are a major global health concern, and they result in millions of deaths every year. Methicillin-resistant Staphylococcus aureus (MRSA) is one of such drug-resistant microbial strains, and new and effective antimicrobial agents are desperately needed to combat infections caused by MRSA. In the search for effective anti-MRSA agents, the leaves of Citrus grandis (Rutaceae), also known as C. maxima, were investigated. Implementing a bioassay-guided approach, sinensetin (2), which is a polymethoxyflavone, was isolated as a promising anti-MRSA compound, showing inhibitory activity against three (EMRSA-15, MRSA340802 and MRSA274819; MIC values 128-256 μg/mL) of five MRSA strains tested in the present study. Five other flavonoids 6,7,8,3',4'-pentamethoxyflavone (1), cirsilineol (3), nobiletin (4), 5-desmethylsinensetin (5) and hesperidin (6) were isolated from the dichloromethane extract of this plant. They displayed varied levels of antimicrobial activities against the tested microbial strains, Micrococcus luteus NCTC 7508, Escherichia coli NCTC 12241 and Pseudomonas aeruginosa NCTC 12903, and a fungal strain, Candida albicans ATCC 90028, but not against Staphylococcus aureus NCTC 12981. Sinensetin (2) also exhibited strong antimicrobial activity against the fungal strain C. albicans with an MIC value of 0.0625 mg/mL. The chemical structures of all isolated compounds were unequivocally elucidated by spectroscopic means (1D and 2D NMR and HR-ESIMS). The present study revealed sinensetin (2) as a potential structural template for generating structural analogues and developing anti-MRSA agents and provided scientific evidence supporting the traditional uses of C. grandis in the treatment of microbial infections.
- Klíčová slova
- Citrus grandis, MRSA, Polymethoxyflavonoids, Rutaceae, Sinensetin, Staphylococcus aureus,
- MeSH
- antibakteriální látky farmakologie izolace a purifikace chemie MeSH
- Candida albicans účinky léků MeSH
- Citrus * chemie MeSH
- flavonoidy * farmakologie izolace a purifikace MeSH
- fytonutrienty farmakologie izolace a purifikace MeSH
- listy rostlin * chemie MeSH
- methicilin rezistentní Staphylococcus aureus * účinky léků MeSH
- mikrobiální testy citlivosti * MeSH
- molekulární struktura MeSH
- rostlinné extrakty farmakologie chemie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky MeSH
- flavonoidy * MeSH
- fytonutrienty MeSH
- rostlinné extrakty MeSH
- sinensetin MeSH Prohlížeč
BACKGROUND: In view of the ever-increasing representation of Staphylococcus spp. strains resistant to various antibiotics, the development of in vivo models for evaluation of novel antimicrobials is of utmost importance. METHODS: In this article, we describe the development of a fully immunocompetent porcine model of extensive skin and soft tissue damage suitable for testing topical antimicrobial agents that matches the real clinical situation. The model was developed in three consecutive stages with protocols for each stage amended based on the results of the previous one. RESULTS: In the final model, 10 excisions of the skin and underlying soft tissue were created in each pig under general anesthesia, with additional incisions to the fascia performed at the base of the defects and immediately inoculated with Staphylococcus aureus suspension. One pig was not inoculated and used as the negative control. Subsequently, the bandages were changed on Days 4, 8, 11, and 15. At these time points, a filter paper imprint technique (FPIT) was made from each wound for semi-quantitative microbiological evaluation. Tissue samples from the base of the wound together with the adjacent intact tissue of three randomly selected defects of each pig were taken for microbiological, histopathological, and molecular-biological examination. The infection with the inoculated S. aureus strains was sufficient during the whole experiment as confirmed by both FPIT and from tissue samples. The dynamics of the inflammatory markers and clinical signs of infection are also described. CONCLUSIONS: A successfully developed porcine model is suitable for in vivo testing of novel short-acting topical antimicrobial agents.
- Klíčová slova
- Staphylococcus aureus, animal model, antimicrobial agents, porcine model, skin and soft‐tissue infection (SSTI), wound infection,
- MeSH
- antibakteriální látky * aplikace a dávkování terapeutické užití farmakologie MeSH
- aplikace lokální MeSH
- infekce měkkých tkání * farmakoterapie mikrobiologie MeSH
- kůže mikrobiologie patologie MeSH
- methicilin rezistentní Staphylococcus aureus * účinky léků MeSH
- modely nemocí na zvířatech * MeSH
- prasata MeSH
- stafylokokové infekce kůže * farmakoterapie mikrobiologie MeSH
- stafylokokové infekce * farmakoterapie mikrobiologie MeSH
- Staphylococcus aureus * účinky léků MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky * MeSH
The preparation of a solid dosage form containing bacteriophages, which meets pharmaceutical requirements and ensures long-term stability of the phage effect, is significant for implementing phage therapy in practice. A commonly used method for processing phages into a solid form is freeze-drying into a so-called freeze-dried cake; however, to date there have been no studies examining the pharmacopeial parameters of freeze-dried tablets with bacteriophages. In this study, we describe the preparation and properties of freeze-dried tablets containing a cocktail of purified pseudomonal bacteriophage DSM 33593 from the genus Pbunavirus and staphylococcal bacteriophage DSM 33473 from the genus Kayvirus (108 PFU/tablet) as the active ingredient. Maltodextrin was used as a tablet filler, and D-mannitol was used as a cryoprotectant. The tablet preparation process resulted in a decrease in phage titer by no more than 1 log PFU/mL. For Pbunavirus, the titer values in tablet and liquid form were comparable. Kayvirus was more stable in tablet form than in liquid form after six months of storage at 25 °C (a decrease of 1.9 ± 0.8 log PFU/mL and 3.8 ± 0.7 log PFU/mL, respectively). The uniformity of mass of single-dose preparations, uniformity of content of single-dose preparations, and their disintegration complied with pharmacopeial requirements. The uniformity of dosage units of the tablets was maintained over three months. A microscopic examination of the internal part of the tablet revealed a heterogeneous structure, which does not affect the required pharmacopeial properties of the tablets. This study highlights the potential of freeze-dried tablets for long-term preservation of the phage effect at room temperature.
- Klíčová slova
- D-mannitol (PubChem CID: 6251), Disodium hydrogen phosphate (PubChem CID: 24203), Dosage form, Drug stability, Freeze-dried tablets, Kayvirus, Magnesium sulfate heptahydrate (PubChem CID: 24843), Maltodextrin (PubChem CID: 68229136), Pbunavirus, Phage therapy, Potassium chloride (PubChem CID: 4873), Potassium dihydrogen phosphate (PubChem CID: 516951), Sodium chloride (PubChem CID: 5234), Sodium deoxycholate (PubChem CID: 23668196), Tris hydrochloride (PubChem CID: 93573), Trisodium citrate (PubChem CID: 6224),
- MeSH
- bakteriofágy MeSH
- fágová terapie metody MeSH
- fágy pseudomonád MeSH
- lyofilizace * MeSH
- mannitol chemie MeSH
- polysacharidy chemie MeSH
- příprava léků metody MeSH
- Pseudomonas aeruginosa * účinky léků virologie MeSH
- stabilita léku MeSH
- stafylokokové bakteriofágy MeSH
- Staphylococcus aureus * účinky léků virologie MeSH
- tablety * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- maltodextrin MeSH Prohlížeč
- mannitol MeSH
- polysacharidy MeSH
- tablety * MeSH
Medical students are exposed to the hospital environment and patients during their studies, increasing the risk of exposure to virulent and antibiotic-resistant isolates of Staphylococcus aureus. The aim of the study is to determine the prevalence of Staphylococcus aureus among medical students who have varying levels of exposure to the hospital environment to provide valuable insights into the risk of colonization and transmission. Nasal swabs and fingerprints were obtained and cultured on a selective medium for staphylococci. The obtained isolates were confirmed as methicillin-sensitive S. aureus (MSSA) or methicillin-resistant (MRSA) using PCR. Antibiotic resistance, the presence of virulence genes including enterotoxin encoding genes, and spa typing were performed. Among pre-clinical students, MSSA was detected on the nose in 45.2% and on the fingerprints in 10.6% of the participants. Among clinical students, MSSA was detected on the nose in 42.0% and on the fingerprints in 25.4%. Only one MRSA isolate was obtained. Genes seg and sei were the most frequently detected in both student groups, with their presence in over 40% of isolates among clinical students. The eta and etb genes were mainly detected from the nose in both student groups. In pre-clinical students, S. aureus carrying eta gene occurred in 6.4% and etb in 8.5%. In clinical students, the occurrence was 5.1% for eta and 8.5% for etb. The tst gene was identified only in the nose and fingerprints of the clinical student group. The most frequently observed resistance was to clindamycin and erythromycin. In total 58 different spa types were identified. High rates of asymptomatic MSSA carriage were observed in both groups of medical students. Detected MSSA strains showed a high degree of genetic variability, with a number of them carrying the virulence and antibiotic resistance genes. Although students do not exhibit increased risk to their patient's, increased hygiene is required in asymptomatic carriage personnel. The overall prevalence of MRSA was low, with a minimal risk of spread.
- Klíčová slova
- Spa type, Staphylococcus aureus, Antibiotic resistance, MRSA, Medical students, Virulence genes,
- MeSH
- antibakteriální látky farmakologie MeSH
- dospělí MeSH
- faktory virulence * genetika MeSH
- lidé MeSH
- methicilin rezistentní Staphylococcus aureus genetika izolace a purifikace účinky léků klasifikace MeSH
- mikrobiální testy citlivosti MeSH
- mladý dospělý MeSH
- přenašečství * mikrobiologie epidemiologie MeSH
- prevalence MeSH
- stafylokokové infekce * mikrobiologie epidemiologie MeSH
- Staphylococcus aureus * genetika izolace a purifikace účinky léků klasifikace MeSH
- studenti lékařství * MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky MeSH
- faktory virulence * MeSH
Azo dyes are used as coloring agent in textile industries at larger scale. As a result, large quantity of dye-enriched waste water is generated which subsequently poses environmental problems. Biological tool involving bacteria having azoreductase enzyme has proved to be more effective and efficient in dye effluent treatment. Current work focuses on Staphylococcus caprae (S. caprae) for degradation and decolorization of Reactive Red-195 (RR-195) azo dye. For this purpose, factors such as pH, temperature, inoculums, carbon and nitrogen sources, and dye concentrations have been optimized for maximum decolorization and degradation. S. caprae (4 mg/mL) efficiently resulted into 90% decolorization of RR-195 dye under static condition at 100 µg/mL concentration, 30 °C and pH 7.0 at a 12-h contact period. FTIR analysis has revealed the formation of new functional groups in the treated dye such as O-H stretch at 3370 cm-1, C-H band stretching at 2928 cm-1, and new band at 1608 cm-1 which specify the degradation of aromatic ring, 1382 and 1118 cm-1 represents desulfonated peaks. Biodegraded metabolites of RR-195 dye such as phenol, 3, 5-di-tert-butylphenol, and phthalic acid have been identified respectively that find industrial applications. Phytotoxicity test has shown non-toxic effects of treated dye on germination of Vigna radiata and Triticum aestivum seeds. Further, antibiotic diffusion assay has confirmed the biosafety of S. caprae.
- Klíčová slova
- Staphylococcus caprae, Biodegradation, GC–MS, RR-195 dye, Toxicity reduction,
- MeSH
- azosloučeniny * metabolismus MeSH
- barvicí látky * metabolismus chemie MeSH
- biodegradace MeSH
- chemické látky znečišťující vodu * metabolismus MeSH
- dusík metabolismus MeSH
- koncentrace vodíkových iontů MeSH
- naftalensulfonany * metabolismus MeSH
- odpadní voda * mikrobiologie chemie MeSH
- průmyslový odpad MeSH
- Staphylococcus * metabolismus izolace a purifikace genetika MeSH
- teplota MeSH
- textilie MeSH
- textilní průmysl MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- azosloučeniny * MeSH
- barvicí látky * MeSH
- chemické látky znečišťující vodu * MeSH
- dusík MeSH
- naftalensulfonany * MeSH
- odpadní voda * MeSH
- průmyslový odpad MeSH
Antibiotic-resistant strains of Staphylococcus aureus pose a significant threat in healthcare, demanding urgent therapeutic solutions. Combining bacteriophages with conventional antibiotics, an innovative approach termed phage-antibiotic synergy, presents a promising treatment avenue. However, to enable new treatment strategies, there is a pressing need for methods to assess their efficacy reliably and rapidly. Here, we introduce a novel approach for real-time monitoring of pathogen lysis dynamics employing the piezoelectric quartz crystal microbalance (QCM) with dissipation (QCM-D) technique. The sensor, a QCM chip modified with the bacterium S. aureus RN4220 ΔtarM, was utilized to monitor the activity of the enzyme lysostaphin and the phage P68 as model lytic agents. Unlike conventional QCM solely measuring resonance frequency changes, our study demonstrates that dissipation monitoring enables differentiation of bacterial growth and lysis caused by cell-attached lytic agents. Compared to reference turbidimetry measurements, our results reveal distinct alterations in the growth curve of the bacteria adhered to the sensor, characterized by a delayed lag phase. Furthermore, the dissipation signal analysis facilitated the precise real-time monitoring of phage-mediated lysis. Finally, the QCM-D biosensor was employed to evaluate the synergistic effect of subinhibitory concentrations of the antibiotic amoxicillin with the bacteriophage P68, enabling monitoring of the lysis of P68-resistant wild-type strain S. aureus RN4220. Our findings suggest that this synergy also impedes the formation of bacterial aggregates, the precursors of biofilm formation. Overall, this method brings new insights into phage-antibiotic synergy, underpinning it as a promising strategy against antibiotic-resistant bacterial strains with broad implications for treatment and prevention.
- Klíčová slova
- Staphylococcus aureus, Antimicrobial treatment, Multidrug-resistant bacteria, Phage therapy, Phage-antibiotic synergy, Piezoelectric biosensor,
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriofágy MeSH
- biosenzitivní techniky * metody MeSH
- lysostafin farmakologie MeSH
- mikrorovnovážné techniky křemenného krystalu * MeSH
- Staphylococcus aureus * účinky léků MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antibakteriální látky MeSH
- lysostafin MeSH
