Macrococci are usually found as commensals on the skin and mucosa of animals and have been isolated from mammal-derived fermented foods; however, they can also act as opportunistic pathogens. Here, we used whole-genome sequencing, comparative genomics, extensive biotyping, MALDI-TOF mass spectrometry, and chemotaxonomy to characterize Macrococcus sp. strains isolated from livestock and human-related specimens. Based on the results of polyphasic taxonomy, we propose the species Macrococcus psychrotolerans sp. nov. (type strain NRL/St 95/376T = CCM 8659T = DSM 111350T) belonging to the Macrococcus caseolyticus phylogenetic clade. It grows at 4°C, and the core genome of the isolates contains suspected genes contributing to low-temperature tolerance. Variable genetic elements include prophages, chromosomal islands, a composite staphylococcal cassette chromosome island, and many plasmids that affect the overall genome expansion and adaptation to specific ecological settings of the studied isolates. Large plasmids carrying the methicillin resistance gene mecB were identified in M. psychrotolerans sp. nov. strains and confirmed as self-transmissible to Staphylococcus aureus in vitro. In addition to plasmids with circular topology, a 150-kb-long linear plasmid with 14.1-kb-long inverted terminal repeats, harboring many IS elements and putative genes for a type IV secretion system was revealed. The described strains were isolated from human clinical material, food-producing animals, meat, and a wooden cheese board and have the potential to proliferate at refrigerator temperatures. Their presence in the food chain and human infections indicates that attention needs to be paid to this potential novel opportunistic pathogen.IMPORTANCEThe study offers insights into the phenotypic and genomic features of a novel species of the genus Macrococcus that occurs in livestock, food, and humans. The large number of diverse mobile genetic elements contributes to the adaptation of macrococci to various environments. The ability of the described microorganisms to grow at refrigerator temperatures, enabled by genes that are predicted to contribute to low-temperature tolerance, raises food safety concerns. Confirmed in vitro conjugative transfer of plasmid-borne mecB gene to S. aureus poses a significant risk of spread of broad β-lactam resistance. In addition, the intergeneric plasmid transfer to S. aureus is indicative of horizontal gene transfer events that may be more frequent than generally accepted. Determining a complete sequence and gene content of linear megaplasmid with exceptional topology for the Staphylococcaceae family suggests its possible role in shuttling adaptive traits through an exchange of genetic information.

• Klíčová slova
• Gram-positive cocci, cephalosporin resistance, cold temperature tolerance, conjugation, food safety, linear plasmid, methicillin resistance,
• MeSH
• antibakteriální látky * farmakologie   MeSH
• bakteriální proteiny * genetika   metabolismus   MeSH
• beta-laktamová rezistence * genetika   MeSH
• dobytek mikrobiologie   MeSH
• Enterococcaceae * genetika   izolace a purifikace   účinky léků   MeSH
• fylogeneze MeSH
• genom bakteriální MeSH
• lidé MeSH
• plazmidy MeSH
• přenos genů horizontální MeSH
• sekvenování celého genomu MeSH
• stafylokokové infekce mikrobiologie   MeSH
• Staphylococcus aureus * genetika   účinky léků   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky * MeSH
• bakteriální proteiny * MeSH

Selenium is an essential trace element that exists in inorganic forms (selenite and selenates) and organic forms (selenoamino acids, seleno peptides, and selenoproteins). Selenium is known to aid in the function of the immune system for populations where human immunodeficiency virus (HIV) is endemic, as studies suggest that a lack of selenium is associated with a higher risk of mortality among those with HIV. In a recent study conducted in Zambia, adults had a median plasma selenium concentration of 0.27 μmol/L (IQR 0.14-0.43). Concentrations consistent with deficiency (<0.63 μmol/L) were found in 83% of adults. With these results, it can be clearly seen that selenium levels in Southern Africa should be investigated to ensure the good health of both livestock and humans. The recommended selenium dietary requirement of most domesticated livestock is 0.3 mg Se/kg, and in humans above 19 years, anRDA (recommended daily allowance) of 55 mcg Se/per dayisis recommended, but most of the research findings of Southern African countries have recorded low levels. With research findings showing alarming low levels of selenium in soils, humans, and raw feed materials in Southern Africa, further research will be vital in answering questions on how best to improve the selenium status of Southern African soils and plants for livestock and humans to attain sufficient quantities.

• Klíčová slova
• Southern Africa, biofortification, feed, food, humans, livestock, nutrition, selenium,
• MeSH
• dobytek MeSH
• dospělí MeSH
• HIV infekce * MeSH
• lidé MeSH
• půda MeSH
• selen * MeSH
• zvířata MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Geografické názvy
• jižní Afrika MeSH
• Zambie MeSH
• Názvy látek
• půda MeSH
• selen * MeSH

ParSCo (Parasitology Summer Course) is an intense, 1-week-long summer course organized by the Parasitology Unit of the Department of Veterinary Medicine, University of Bari, Italy, with the support of the World Association for the Advancement of Veterinary Parasitology (WAAVP), the European Veterinary Parasitology College (EVPC) and Parasites and Vectors. The course, which is conducted in southern Italy, is planned for parasitologists and post-graduate students working in the field of parasitology. The course consists of theoretical and practical lessons, which include the collection, identification and diagnosis of parasites of pets, livestock and wildlife. The participants in ParSCo are afforded the opportunity to be involved in clinical examination and sample collection for the diagnosis of parasitic diseases (e.g. leishmaniosis, thelaziosis and many tick-borne diseases) present in the Mediterranean Basin. The course is conducted at Casa di Caccia, a hunting lodge situated in the Gallipoli Cognato Forest near the Basento River in the Basilicata region in southern Italy. In addition to the training purpose, ParSCo is a great opportunity for sharing knowledge and expertise while becoming part of the parasitology community in a pleasant environment. In this editorial, we share some information and celebrate 10 years of ParSCo, looking forward to forthcoming sessions of this unique parasitology summer course.

• MeSH
• divoká zvířata MeSH
• dobytek MeSH
• lidé MeSH
• parazitární nemoci u zvířat * parazitologie   MeSH
• parazitologie MeSH
• studenti MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• úvodníky MeSH
• Geografické názvy
• Itálie epidemiologie   MeSH

Livestock depredation by leopards is a pervasive issue across many Asian and African range countries, particularly in and around protected areas. Developing effective conflict mitigation strategies requires understanding the landscape features influencing livestock depredation. In this study, we investigated predictors associated with livestock depredation by leopards using 274 cases of leopard attacks on livestock that occurred between 2017 and 2020 in the Annapurna Conservation Area, Nepal. We also examined how livestock predation by leopards varied depending on the species, season, and time. A generalized linear model with binary logistic regression was used to test the statistical significance of variables associated with the presence and absence of conflict sites. The results revealed that the area of forest, agricultural land, length of rivers, slope, proximity to settlements and protected areas, and elevation significantly predicted the probability of leopard attacks on livestock. We also observed a significant increase in the incidence of leopard predation on livestock with decreasing slopes and rising elevations. The areas near human settlements and the protected areas faced a higher risk of leopard predation. The incidence of leopard predation on livestock varied significantly depending on the livestock species, season, and time. Goats were the most highly predated livestock, followed by sheep, cow/ox, and buffalo. A total of 289.11 km2 (or around 5% of the research area) was deemed to be at high risk for leopard predation on livestock. This study's comprehensive understanding of human-leopard conflicts provides valuable insights for planning and implementing measures to reduce damage caused by leopard populations throughout their range.

• Klíčová slova
• Annapurna Conservation Area, Landscape predictors, Livestock depredation,
• MeSH
• dobytek * MeSH
• ekosystém MeSH
• kozy MeSH
• lidé MeSH
• ovce MeSH
• Panthera * MeSH
• skot MeSH
• zachování přírodních zdrojů MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• skot MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Nepál MeSH

Grazing represents the most extensive use of land worldwide. Yet its impacts on ecosystem services remain uncertain because pervasive interactions between grazing pressure, climate, soil properties, and biodiversity may occur but have never been addressed simultaneously. Using a standardized survey at 98 sites across six continents, we show that interactions between grazing pressure, climate, soil, and biodiversity are critical to explain the delivery of fundamental ecosystem services across drylands worldwide. Increasing grazing pressure reduced ecosystem service delivery in warmer and species-poor drylands, whereas positive effects of grazing were observed in colder and species-rich areas. Considering interactions between grazing and local abiotic and biotic factors is key for understanding the fate of dryland ecosystems under climate change and increasing human pressure.

• MeSH
• biodiverzita * MeSH
• býložravci * MeSH
• dobytek * MeSH
• klimatické změny MeSH
• půda MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, U.S. Gov't, Non-P.H.S. MeSH
• Názvy látek
• půda MeSH

This study was aimed on the detection of methicillin resistant Staphylococcus aureus (MRSA) in different categories of retailed ready-to-eat (RTE) meat products from the Czech producers and determination of their genetic properties, antimicrobial resistance and virulence. In RTE meat products, 2% (4/181) of examined samples were MRSA positive. MRSA strains were detected only in durable fermented meat products made exclusively from pork meat. Detection of livestock-associated MRSA (LA-MRSA) clonal lineages (ST398 and ST4999), SCCmec cassette type V and tetracycline resistance indicate a source of contamination from raw pork. The study confirms the ability of these strains to survive the technological process rather than contamination of meat products from the food processing environment. MRSA strains did not carry any of the tested genes encoding staphylococcal enterotoxins or virulence genes (for Panton-Valentine leukocidin, exfoliative toxins A, B and toxic shock syndrome). Our results point out the spread of LA-MRSA through the meat processing chain.

• Klíčová slova
• Clonal lineages, Fermented meat product, Livestock-associated MRSA, Meat chain, Resistance, Virulence,
• MeSH
• antibakteriální látky farmakologie   MeSH
• dobytek MeSH
• masné výrobky * MeSH
• maso MeSH
• methicilin rezistentní Staphylococcus aureus * genetika   MeSH
• mikrobiální testy citlivosti MeSH
• stafylokokové infekce * MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH
• Názvy látek
• antibakteriální látky MeSH

Stocking rate and rangeland area are key variables to provide the livelihood of herders in different climatic zones. To evaluate the economic and ecological management of pastoral units, this study aims to determine the optimal economic size of pastoral units for livestock grazing use considering the ecological capacity of semi-arid rangelands in different climatic scenarios. Therefore, 12 pastoral units (an area of 47,355 ha) were selected in two climatic zones (summer and winter rangelands) in the Kalat region of Razavi Khorasan Province, Iran. By measuring forage production, carrying capacity was calculated. Based on the results, the costs of traditional livestock management in winter rangelands are higher than those of summer rangelands. Moreover, the current size of the assigned rangeland, especially the summer rangeland, is lower than that of the economic justification. The results emphasize that rangeland-based livestock husbandry cannot create a good livelihood for herders in the region, and it is necessary to pay special attention to other services and aspects, despite the existing ecological and socio-economic complexities. In this regard, providing multi-purpose rangeland use and useable technologies to better manage these areas is necessary to increase per capita household income and reduce the stocking rate in the region's rangelands. Ultimately, both increasing the level of available rangelands and reducing costs by applying new technology are required, as is the economic consideration of pastoral units by using rangelands for multiple purposes.

• Klíčová slova
• Forage production, Herders' livelihood, Summer and winter rangelands, Traditional livestock management,
• MeSH
• dobytek * MeSH
• roční období MeSH
• zachování přírodních zdrojů * MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Írán MeSH

Mediterranean pastures are experiencing strong changes in management, involving shifts from sheep to cattle-based livestock systems. The impacts of such shifts on biodiversity are still poorly understood. Here, we sought to contrast the grazing regime, vegetation structure, bird species richness and abundance, between sheep and cattle grazed parcels, to understand the mechanisms through which management decisions impact farmland birds. During spring 2019, we characterized livestock management, bird populations and sward structure in 23 cattle and 27 sheep grazed parcels. We used a Structural Equation Model to infer the direct and indirect effects of sheep and cattle grazing on birds. Although no effects were found on overall species richness, there were species-specific responses to sheep and cattle grazed systems. Grazing pressure (variable integrating stocking rate and the number of days in the parcel) had negative impacts on the prevalence/abundance of Zitting Cisticola, Corn Bunting and Little Bustard, either directly or indirectly, through the effects of grazing pressure on vegetation height. Animal density and vegetation cover had direct positive effects in Galerida spp. and Common Quail, respectively. Zitting Cisticola and Little Bustard also showed a direct response to livestock type. Our study emphasizes the importance of grazing pressure as a driver of negative impacts for bird populations in Mediterranean grasslands. Since the ongoing transition from sheep to cattle-based systems involves increases in stocking rate, and therefore potentially higher grazing pressure, we propose a policy change to cap the maximum allowed grazing pressure. At the landscape scale, a mix of sheep and cattle grazed fields would be beneficial for maintaining bird diversity.

• MeSH
• biodiverzita MeSH
• býložravci MeSH
• dobytek klasifikace   růst a vývoj   MeSH
• ovce MeSH
• pastviny MeSH
• ptáci klasifikace   růst a vývoj   MeSH
• skot MeSH
• teoretické modely MeSH
• zachování přírodních zdrojů MeSH
• zemědělství metody   MeSH
• zvířata MeSH
• Check Tag
• skot MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Středomoří MeSH

According to Darwin's theory, endless evolution leads to a revolution. One such example is the Clustered Regularly Interspaced Palindromic Repeats (CRISPR)-Cas system, an adaptive immunity system in most archaea and many bacteria. Gene editing technology possesses a crucial potential to dramatically impact miscellaneous areas of life, and CRISPR-Cas represents the most suitable strategy. The system has ignited a revolution in the field of genetic engineering. The ease, precision, affordability of this system is akin to a Midas touch for researchers editing genomes. Undoubtedly, the applications of this system are endless. The CRISPR-Cas system is extensively employed in the treatment of infectious and genetic diseases, in metabolic disorders, in curing cancer, in developing sustainable methods for fuel production and chemicals, in improving the quality and quantity of food crops, and thus in catering to global food demands. Future applications of CRISPR-Cas will provide benefits for everyone and will save countless lives. The technology is evolving rapidly; therefore, an overview of continuous improvement is important. In this review, we aim to elucidate the current state of the CRISPR-Cas revolution in a tailor-made format from its discovery to exciting breakthroughs at the application level and further upcoming trends related to opportunities and challenges including ethical concerns.

• Klíčová slova
• CRISPR/Cas9, agricultural production, genome editing, industrial applications, livestock, therapeutics,
• MeSH
• Archaea metabolismus   MeSH
• Bacteria metabolismus   MeSH
• CRISPR-Cas systémy * MeSH
• dějiny 20. století MeSH
• dějiny 21. století MeSH
• dobytek MeSH
• editace genu metody   MeSH
• genetické inženýrství dějiny   metody   MeSH
• genom MeSH
• lidé MeSH
• sekvence CRISPR MeSH
• zemědělské plodiny genetika   MeSH
• zvířata MeSH
• Check Tag
• dějiny 20. století MeSH
• dějiny 21. století MeSH
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• historické články MeSH
• přehledy MeSH

Mycotoxins are secondary metabolites produced by several species of fungi, including the Fusarium, Aspergillus, and Penicillium species. Currently, more than 300 structurally diverse mycotoxins are known, including a group called minor mycotoxins, namely enniatins, beauvericin, and fusaproliferin. Beauvericin and enniatins possess a variety of biological activities. Their antimicrobial, antibiotic, or ionoforic activities have been proven and according to various bioassays, they are believed to be toxic. They are mainly found in cereal grains and their products, but they have also been detected in forage feedstuff. Mycotoxins in feedstuffs of livestock animals are of dual concern. First one relates to the safety of animal-derived food. Based on the available data, the carry-over of minor mycotoxins from feed to edible animal tissues is possible. The second concern relates to detrimental effects of mycotoxins on animal health and performance. This review aims to summarize current knowledge on the relation of minor mycotoxins to livestock animals.

• Klíčová slova
• beauvericin, carry-over, enniatins, feed, metabolism, minor mycotoxins,
• MeSH
• depsipeptidy toxicita   MeSH
• dobytek MeSH
• jedlá semena toxicita   MeSH
• kontaminace potravin analýza   MeSH
• krmivo pro zvířata toxicita   MeSH
• mykotoxiny * MeSH
• terpeny MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• beauvericin MeSH Prohlížeč
• depsipeptidy MeSH
• enniatins MeSH Prohlížeč
• fusaproliferin MeSH Prohlížeč
• mykotoxiny * MeSH
• terpeny MeSH