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Autor: Janeckova, E

9 záznamů v PubMed Filtry

Článek

Role of c-Myb in chondrogenesis

Oralová, V
Autor Oralová, V Institute of Animal Physiology and Genetics CAS, v.v.i., Brno, Czech Republic; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic. Electronic address: 211812@mail.muni.cz
Matalová, E
Autor Matalová, E Institute of Animal Physiology and Genetics CAS, v.v.i., Brno, Czech Republic; Department of Physiology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
Janečková, E
Autor Janečková, E Institute of Animal Physiology and Genetics CAS, v.v.i., Brno, Czech Republic; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic
Drobná Krejčí, E
Autor Drobná Krejčí, E Institute of Anatomy, Charles University, Prague, Czech Republic
Knopfová, L
Autor Knopfová, L Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic
Šnajdr, P
Autor Šnajdr, P Institute of Anatomy, Charles University, Prague, Czech Republic
Tucker, A S
Autor Tucker, A S Department of Craniofacial Development and Stem Cell Biology, King's College London, London, UK
Veselá, I
Autor Veselá, I Department of Anatomy, Histology and Embryology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
Šmarda, J
Autor Šmarda, J Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic
Buchtová, M
Autor Buchtová, M Institute of Animal Physiology and Genetics CAS, v.v.i., Brno, Czech Republic; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic

Bone. 2015 Jul ; 76 () : 97-106. [epub] 20150404

ISSN 1873-2763
Zdroj

The Myb locus encodes the c-Myb transcription factor involved in controlling a broad variety of cellular processes. Recently, it has been shown that c-Myb may play a specific role in hard tissue formation; however, all of these results were gathered from an analysis of intramembranous ossification. To investigate a possible role of c-Myb in endochondral ossification, we carried out our study on the long bones of mouse limbs during embryonic development. Firstly, the c-myb expression pattern was analyzed by in situ hybridization during endochondral ossification of long bones. c-myb positive areas were found in proliferating as well as hypertrophic zones of the growth plate. At early embryonic stages, localized expression was also observed in the perichondrium and interdigital areas. The c-Myb protein was found in proliferating chondrocytes and in the perichondrium of the forelimb bones (E14.5-E17.5). Furthermore, protein was detected in pre-hypertrophic as well as hypertrophic chondrocytes. Gain-of-function and loss-of-function approaches were used to test the effect of altered c-myb expression on chondrogenesis in micromass cultures established from forelimb buds of mouse embryos. A loss-of-function approach using c-myb specific siRNA decreased nodule formation, as well as downregulated the level of Sox9 expression, a major marker of chondrogenesis. Transient c-myb overexpression markedly increased the formation of cartilage nodules and the production of extracellular matrix as detected by intense staining with Alcian blue. Moreover, the expression of early chondrogenic genes such as Sox9, Col2a1 and activity of a Col2-LUC reporter were increased in the cells overexpressing c-myb while late chondrogenic markers such as Col10a1 and Mmp13 were not significantly changed or were downregulated. Taken together, the results of this study demonstrate that the c-Myb transcription factor is involved in the regulation and promotion of endochondral bone formation.

Klíčová slova
Chondrogenesis, Endochondral bone, Micromass cultures, Mouse limbs, Up- and down-regulation, siRNA,
MeSH
biologické markery metabolismus MeSH
buněčná diferenciace MeSH
chondrogeneze fyziologie MeSH
hybridizace in situ MeSH
končetiny embryologie MeSH
myši MeSH
protoonkogenní proteiny c-myb genetika fyziologie MeSH
umlčování genů MeSH
zvířata MeSH
Check Tag
myši MeSH
zvířata MeSH
Publikační typ
časopisecké články MeSH
práce podpořená grantem MeSH
Názvy látek
biologické markery MeSH
protoonkogenní proteiny c-myb MeSH

Článek

Cell proliferation and apoptosis in the primary enamel knot measured by flow cytometry of laser microdissected samples

Archives of oral biology. 2010 Aug ; 55 (8) : 570-5. [epub] 20100615

Arch Oral Biol
ISSN 1879-1506 | 0003-9969
Zdroj

Laser capture microdissection (LCM) uniquely allows the selection of specific cell populations from histological sections. These selected cells are then catapulted into a test tube without any contamination from surrounding tissues. During the last ten years, many significant results have been achieved, particularly at the level of DNA and RNA where amplification techniques are available. However, where amplification procedures are difficult, the benefits of LCM diminish. To overcome such difficulties, a novel approach, combining laser capture microdissection and flow cytometry, has been tested here for detection of apoptosis and proliferation in tissue bound cell populations without any amplification steps. The mouse cap stage molar tooth germ was used as a model. At the centre of the inner enamel epithelium, the primary enamel knot is a clearly defined apoptotic population with minimal proliferation, flanked by the highly proliferative cervical loops on each side. Thus within the tooth germ epithelium at this stage, two distinct populations of cells are found side by side. These populations were selected by laser capture microdissection and then analysed by flow cytometry for apoptosis and proliferation. Flow cytometric results correlated well with immunohistochemical findings, demonstrating the success and sensitivity of this combined procedure.

MeSH
apoptóza fyziologie MeSH
epitelové buňky cytologie MeSH
gestační stáří MeSH
imunohistochemie MeSH
koncové značení zlomů DNA in situ MeSH
kryoprezervace MeSH
laserová terapie metody MeSH
mikrodisekce metody MeSH
moláry embryologie MeSH
myši MeSH
orgán skloviny cytologie MeSH
počet buněk MeSH
proliferace buněk MeSH
proliferační antigen buněčného jádra analýza MeSH
průtoková cytometrie * MeSH
senzitivita a specificita MeSH
zubní krček cytologie embryologie MeSH
zubní zárodek cytologie MeSH
zvířata MeSH
Check Tag
myši MeSH
zvířata MeSH
Publikační typ
časopisecké články MeSH
práce podpořená grantem MeSH
Názvy látek
proliferační antigen buněčného jádra MeSH

Článek

Intenzívní psychiatrická péce o nemocné s psychogenními poruchami príjmu potravy
[Intensive psychiatric care of patients with psychogenic eating disorders]

Ceskoslovenska psychiatrie. 1992 Jun ; 88 (2) : 91-6.

Cesk Psychiatr
ISSN 0069-2336
Zdroj

The authors summarize their therapeutic results in anorexia nervosa achieved at the unit of specialized care for eating disorders at the Psychiatric Clinic of the First Medical Faculty, Charles University, Prague. They find that applications for hospitalization of these patients have a rising trend and that in recent years in the unit mainly patients with severe forms of these diseases are admitted. During the past 7 years in the unit a total of 147 patients were hospitalized. By comprehensive regime treatment 84% of the patients with bulimia nervosa. As to basic symptoms, in bulimia nervosa the results were achieved in vomiting and bulimic attacks and in anorexia nervosa as regards appetite, hunger and general attitude to food. Finally the authors summarize the advantages of the unit specialized care for psychogenic eating disorders.