Human dental pulp stem cells (hDPSCs) are mesenchymal stem cells that have been successfully used in human bone tissue engineering. To establish whether these cells can lead to a bone tissue ready to be grafted, we checked DPSCs for their osteogenic and angiogenic differentiation capabilities with the specific aim of obtaining a new tool for bone transplantation. Therefore, hDPSCs were specifically selected from the stromal-vascular dental pulp fraction, using appropriate markers, and cultured. Growth curves, expression of bone-related markers, calcification and angiogenesis as well as an in vivo transplantation assay were performed. We found that hDPSCs proliferate, differentiate into osteoblasts and express high levels of angiogenic genes, such as vascular endothelial growth factor and platelet-derived growth factor A. Human DPSCs, after 40 days of culture, give rise to a 3D structure resembling a woven fibrous bone. These woven bone (WB) samples were analysed using classic histology and synchrotron-based, X-ray phase-contrast microtomography and holotomography. WB showed histological and attractive physical qualities of bone with few areas of mineralization and neovessels. Such WB, when transplanted into rats, was remodelled into vascularized bone tissue. Taken together, our data lead to the assumption that WB samples, fabricated by DPSCs, constitute a noteworthy tool and do not need the use of scaffolds, and therefore they are ready for customized regeneration.

• Klíčová slova
• bone differentiation, bone regeneration, bone tissue engineering, hDPSCs, holotomography, human Dental Pulp Stem Cells, human serum, phc-microCT, woven bon, woven bone,
• MeSH
• buněčná diferenciace fyziologie   MeSH
• chemotaxe MeSH
• dospělí MeSH
• fyziologická kalcifikace fyziologie   MeSH
• fyziologická neovaskularizace fyziologie   MeSH
• kmenové buňky cytologie   MeSH
• kostní náhrady * MeSH
• kultivované buňky MeSH
• lidé MeSH
• mladý dospělý MeSH
• myši nahé MeSH
• osteogeneze fyziologie   MeSH
• osteokalcin metabolismus   MeSH
• proliferace buněk MeSH
• rentgenová mikrotomografie metody   MeSH
• separace buněk metody   MeSH
• tkáňové inženýrství metody   MeSH
• transplantace kostí metody   MeSH
• zubní dřeň cytologie   MeSH
• zvířata MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladý dospělý MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• Názvy látek
• kostní náhrady * MeSH
• osteokalcin MeSH