The aim of this study was to determine whether lymphocyte apoptosis is modulated by infections caused by Staphylococcus aureus and Streptococcus uberis. Samples of cell populations were obtained by lavage of the mammary glands at 4 intervals (24, 48, 72 and 168 h) following infection. The percentage of apoptotic lymphocytes peaked at 168 h after challenge with S. aureus or S. uberis. Subsequent experiments focused on in vitro cultivation of mammary gland lymphocytes with S. aureus and S. uberis. These experiments showed a lower percentage of apoptotic lymphocytes following 3h of cultivating cells with bacteria than after cultivation without bacteria. The results demonstrate that during both experimental infection of bovine mammary glands with S. aureus or S. uberis and during in vitro cultivation of lymphocytes with S. aureus or S. uberis, apoptosis of lymphocytes is delayed.
- MeSH
- antigeny CD2 analýza MeSH
- antigeny povrchové analýza MeSH
- apoptóza MeSH
- imunoglobulin G analýza MeSH
- lymfocyty cytologie imunologie fyziologie MeSH
- mléčné žlázy zvířat cytologie mikrobiologie fyziologie MeSH
- nemoci skotu imunologie mikrobiologie MeSH
- skot MeSH
- stafylokokové infekce imunologie veterinární MeSH
- Staphylococcus aureus izolace a purifikace MeSH
- Streptococcus izolace a purifikace MeSH
- streptokokové infekce imunologie veterinární MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antigeny CD2 MeSH
- antigeny povrchové MeSH
- imunoglobulin G MeSH
