• MeSH
• biodegradace MeSH
• čištění vody metody   MeSH
• environmentální zdraví statistika a číselné údaje   MeSH
• látky znečišťující vodu analýza   toxicita   MeSH
• mikrobiologie vody MeSH
• testy toxicity metody   MeSH
• vodní organismy účinky léků   fyziologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• úvodní články MeSH
• úvodníky MeSH
• Názvy látek
• látky znečišťující vodu MeSH

1. The purpose of this study was to compare tributyltin (TBT)-induced cytochrome P450 1 (CYP450 1) regulation in liver, gills and muscle of juvenile common carp (Cyprinus carpio). 2. Fish were exposed to sublethal concentrations of TBT (75, 0.75 and 7.5 μg/L) for 60 days. CYP450 1A was measured at the enzyme activity level as 7-ethoxyresorufin-O-deethylase (EROD) activity, as well as the mRNA expression of CYP450 1 family genes (CYP1A, CYP1B, CYP1C1 and CYP1C2) in fish tissues. 3. Based on the results, the liver displayed the highest absolute levels of EROD activity, both under nonexposed and exposed conditions. Additional, EROD activities and CYP1A gene levels showed a good correlation in all three organs. According to the mRNA expression of CYP450 1 family genes, it suggested that CYP1A was to accommodate most EROD activity in fish, but other CYP450 forms also involved in this proceeding. 4. Overall, the study revealed both similarities and differences in the concentration-dependent CYP450 1 responses of the three target organs, which could provide useful information to better understand the mechanisms of TBT-induced bio-toxicity.

• Klíčová slova
• CYP450 1 family genes, EROD, fish, tissue-specific response, tributyltin,
• MeSH
• cytochrom P-450 CYP1A1 metabolismus   MeSH
• kapři genetika   MeSH
• lineární modely MeSH
• messenger RNA genetika   metabolismus   MeSH
• orgánová specificita účinky léků   MeSH
• regulace genové exprese enzymů účinky léků   MeSH
• systém (enzymů) cytochromů P-450 genetika   metabolismus   MeSH
• trialkylcínové sloučeniny toxicita   MeSH
• vystavení vlivu životního prostředí * MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• cytochrom P-450 CYP1A1 MeSH
• messenger RNA MeSH
• systém (enzymů) cytochromů P-450 MeSH
• trialkylcínové sloučeniny MeSH
• tributyltin MeSH Prohlížeč

Tributyltin (TBT) and cadmium (Cd) are two common pollutants in aquatic environments. This study was designed to examine the physiological responses of juvenile Grass Carp Ctenopharyngodon idella to TBT, Cd, and their combination. Fish were apportioned into a control group, a TBT group (7.5 μg/L), a Cd group (2.97 mg/L), and a TBT-Cd group (7.5 μg/L TBT, 2.97 mg/L Cd(2+)) for 7 d. The following activities were measured: Na(+),K(+)-ATPase in gill tissues; nitric oxide synthase (NOS), acetylcholinesterase (AChE), and monoamine oxidase (MAO) in brain tissues; and lipid peroxidation (LPO), malondialdehyde (MDA), total antioxidative capacity (T-AOC), and glutathione (GSH) in liver tissues. Cadmium-induced stress was suggested by alterations in antioxidant responses (MDA, LPO, and T-AOC) and neurological parameters (AChE, MAO, and NOS). Cadmium also induced Na(+),K(+)-ATPase and GSH activity. Compared with the responses among the Cd group, the combination of TBT and Cd not only decreased the level of GSH and Na(+),K(+)-ATPase but also increased the levels of MDA, LPO, AChE, MAO, and NOS. These results suggest that a combination of TBT and Cd could reduce the adverse effects of Cd on Grass Carp. However, the exact mechanisms for the combined effects TBT and Cd on these biomarkers require further investigation. Received September 28, 2015; accepted April 17, 2016.

• MeSH
• antioxidancia metabolismus   MeSH
• biologické markery metabolismus   MeSH
• chemické látky znečišťující vodu toxicita   MeSH
• játra účinky léků   enzymologie   metabolismus   MeSH
• kadmium toxicita   MeSH
• kapři metabolismus   MeSH
• mozek účinky léků   enzymologie   metabolismus   MeSH
• trialkylcínové sloučeniny toxicita   MeSH
• žábry účinky léků   enzymologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antioxidancia MeSH
• biologické markery MeSH
• chemické látky znečišťující vodu MeSH
• kadmium MeSH
• trialkylcínové sloučeniny MeSH
• tributyltin MeSH Prohlížeč

In this study, the chronic toxic effects of tributyltin (TBT), an antifouling paints commonly present in surface and ground water, on morphological indices, reactive oxygen species (ROS) generation, and ATPase activity and heat shock protein (Hsp) 70 protein in tissues (liver, gill, and white muscle) of common carp were investigated. Fish were exposed at sublethal concentrations of TBT (75 ng/L, 0.75 μg/L, and 7.5 μg/L) for 60 days. When compared with the control, there was significant lower condition factor in fish exposed at the higher concentration of TBT. ROS levels in three tissues increased significantly at higher TBT concentrations (0.75 and 7.5 μg/L). The hepatic antioxidant enzymes (total antioxidative capacity and superoxide dismutase) activities were induced at higher concentrations (0.75 μg/L) of TBT. When compared with the hepatic antioxidant enzymes activities in fish exposed to 0.75 μg/L of TBT, there was a decreasing trend in those exposed to TBT with a concentration of 7.5 μg/L. However, all the antioxidant enzymes activities were significantly inhibited in gill and muscle of fish exposed to higher TBT concentrations (0.75 and 7.5 μg/L). Moreover, there was significant lower Na-K-ATPase in three tissues after long-term exposure to higher concentration of TBT, but a significant higher Hsp70 protein levels was observed. In short, environmental concentrations of TBT could not induce obvious impacts on fish, but long-term exposure to higher concentrations of TBT could affect seriously the health status of fish. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 937-944, 2016.

• Klíčová slova
• ATPase activity, ROS generation, fish, morphological indices, tributyltin,
• MeSH
• antioxidancia metabolismus   MeSH
• biologické markery metabolismus   MeSH
• chemické látky znečišťující vodu toxicita   MeSH
• játra účinky léků   enzymologie   MeSH
• kapři metabolismus   MeSH
• orgánová specificita MeSH
• proteiny tepelného šoku HSP70 metabolismus   MeSH
• rybí proteiny metabolismus   MeSH
• sodíko-draslíková ATPasa metabolismus   MeSH
• superoxiddismutasa metabolismus   MeSH
• trialkylcínové sloučeniny toxicita   MeSH
• žábry metabolismus   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antioxidancia MeSH
• biologické markery MeSH
• chemické látky znečišťující vodu MeSH
• proteiny tepelného šoku HSP70 MeSH
• rybí proteiny MeSH
• sodíko-draslíková ATPasa MeSH
• superoxiddismutasa MeSH
• trialkylcínové sloučeniny MeSH
• tributyltin MeSH Prohlížeč

Tributyltin (TBT), as antifouling paints, is widely present in aquatic environment, but little is known regarding the toxicity of TBT on fish brain. In this study, the effects of exposure to TBT on the antioxidant defense system, Na(+) -K(+) -ATPase activity, neurological enzymes activity and Hsp 70 protein level in brain of juvenile common carp (Cyprinus carpio) were studied. Fish were exposed to sublethal concentrations of TBT (5, 10 and 20 μg/L) for 7 days. Based on the results, with increasing concentrations of TBT, oxidative stress was apparent as reflected by the significant higher levels of oxidative indices, as well as the significant inhibition of all antioxidant enzymes activities. Besides, the activities of Acetylcholinesterase (AChE), Monoamine oxidases (MAO) and Na(+) -K(+) -ATPase were significantly inhibited after exposure to TBT with higher concentrations. In addition, the levels of Hsp 70 protein were evaluated under TBT stress with dose-depended manner. These results suggest that selected physiological responses in fish brain could be used as potential biomarkers for monitoring residual organotin compounds present in aquatic environment.

• Klíčová slova
• fish brain, molecular responses, physiological parameters, tributyltin,
• MeSH
• acetylcholinesterasa metabolismus   MeSH
• antioxidancia metabolismus   MeSH
• biologické markery metabolismus   MeSH
• chemické látky znečišťující vodu toxicita   MeSH
• kapři * genetika   metabolismus   MeSH
• mozek účinky léků   metabolismus   MeSH
• oxidační stres účinky léků   MeSH
• sodíko-draslíková ATPasa metabolismus   MeSH
• trialkylcínové sloučeniny toxicita   MeSH
• věkové faktory MeSH
• vodní hospodářství MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• acetylcholinesterasa MeSH
• antioxidancia MeSH
• biologické markery MeSH
• chemické látky znečišťující vodu MeSH
• sodíko-draslíková ATPasa MeSH
• trialkylcínové sloučeniny MeSH
• tributyltin MeSH Prohlížeč

In this study, the effects of tributyltin (TBT) on biochemical parameters (antioxidant responses and Na(+) -K(+) -ATPase) in different tissues were investigated by using juvenile common carp (Cyprinus Carpio) as well as growth and ion regulation-related genes expression and stress-related proteins profiling in fish liver. Oxidative stress indices and Na(+) -K(+) -ATPase showed tissues-specific responses in fish exposed to different TBT concentrations. All tested genes related to GH/IGF-I axis and ion-regulation were significantly induced in the TBT group with lower concentrations (except for the igfbp3 in 10 μg/L) and were inhibited in 20 μg/L. In addition, the profiling of two proteins Hsp 70 and MT were increasing in a dose-dependent manner under TBT stress. In short, TBT-induced biochemical and molecular responses in different tissues were reflected in the measured parameters in the test. On the basis of TBT residue levels in the natural environment, more long-term experiments at lower concentrations will be necessary in the future.

• Klíčová slova
• Common Carp, Gene Expression, Physiological Responses, Protein Profiling, Tributyltin,
• MeSH
• antioxidancia metabolismus   MeSH
• chemické látky znečišťující vodu toxicita   MeSH
• insulinu podobný růstový faktor I biosyntéza   MeSH
• játra účinky léků   metabolismus   MeSH
• kapři růst a vývoj   MeSH
• oxidační stres účinky léků   MeSH
• proteiny tepelného šoku biosyntéza   MeSH
• regulace genové exprese účinky léků   MeSH
• růstový hormon biosyntéza   MeSH
• sodíko-draslíková ATPasa biosyntéza   MeSH
• trialkylcínové sloučeniny toxicita   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antioxidancia MeSH
• chemické látky znečišťující vodu MeSH
• insulinu podobný růstový faktor I MeSH
• proteiny tepelného šoku MeSH
• růstový hormon MeSH
• sodíko-draslíková ATPasa MeSH
• trialkylcínové sloučeniny MeSH
• tributyltin MeSH Prohlížeč

Tributyltin (TBT), a toxic contaminant in aquatic environments, has bio-accumulated in aquatic food webs throughout the world and can be found at toxic levels in some biota. However, the molecular mechanisms and effects of TBT are not fully understood. The aim of the present study was to investigate the effect of long-term exposure of TBT on cytochrome P450 (CYP450) 1 regulation and heat-shock proteins (HSPs) profiling in brain of freshwater teleost. The effects of long-term exposure to TBT on mRNA expression of cytochrome P450 (CYP450) 1 family genes and ethoxyresorufin O-deethylase (EROD) activity in the brain of common carp were evaluated, as well as HSP 70 level. Fish were exposed to sublethal concentrations of TBT (75 ng/L, 0.75 μg/L and 7.5 μg/L) for 15, 30, and 60 days. Based on the results, long-term exposure (more than 15 days) to TBT could lead to obvious physiological-biochemical responses (based on EROD activity, HSP 70 level and CYP450 1 family genes expression). The mRNA expression of CYP450 1 family genes (CYP1A, CYP1B, CYP1C1 and CYP1C2) suggested that CYP1A was to accommodate most EROD activity in fish, but other CYP450 forms also involved in this proceeding. Thus, the measured physiological responses in fish brain could provide useful information to better understand the mechanisms of TBT-induced bio-toxicity and could be used as potential biomarkers for monitoring the TBT pollution in the field.

• Klíčová slova
• CYP450 1 family genes, EROD activity, Fish, Heat-shock proteins, Tributyltin,
• MeSH
• chemické látky znečišťující vodu toxicita   MeSH
• exprese genu účinky léků   MeSH
• kapři MeSH
• messenger RNA metabolismus   MeSH
• mozek účinky léků   metabolismus   MeSH
• proteiny tepelného šoku HSP70 genetika   metabolismus   MeSH
• systém (enzymů) cytochromů P-450 genetika   metabolismus   MeSH
• trialkylcínové sloučeniny toxicita   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• chemické látky znečišťující vodu MeSH
• messenger RNA MeSH
• proteiny tepelného šoku HSP70 MeSH
• systém (enzymů) cytochromů P-450 MeSH
• trialkylcínové sloučeniny MeSH
• tributyltin MeSH Prohlížeč

In the present study, the antioxidant response and acetylcholinesterase (AChE) activity were measured in Chinese rare minnow larvae (Gobiocypris rarus) after exposure to tributyltin (TBT) (0, 100, 400 and 800 ngL(-1)) for 7 days, as well as the expression of a series of genes, including cr, aptase and prl genes involved in the ion-regulatory process and igfbp3 and gh related to growth rate. Results shows that oxidative stress was generated in fish exposed to TBT, as evidenced by elevated malondialdehyde levels and the inhibition of antioxidant parameters. The activity of acetylcholinesterase (AChE) was also inhibited in fish under higher TBT stress. Moreover, genes involved in ion regulation and growth were affected, based on the regulated transcription of the cr, atpase, gh, prl and igfbp3 genes in the treated groups. The observed effects of TBT upon antioxidant responses and altered expression of genes provides insight into the use of these molecular biomarkers in evaluating mechanisms of TBT toxicity in fish.

• Klíčová slova
• Chinese rare minnow, Gene expression, Physiological responses, Tributyltin,
• MeSH
• antioxidancia metabolismus   MeSH
• biologické markery metabolismus   MeSH
• chemické látky znečišťující vodu farmakokinetika   toxicita   MeSH
• Cyprinidae genetika   metabolismus   fyziologie   MeSH
• endokrinní disruptory farmakokinetika   toxicita   MeSH
• larva účinky léků   metabolismus   MeSH
• malondialdehyd metabolismus   MeSH
• monitorování životního prostředí metody   MeSH
• oxidační stres účinky léků   MeSH
• regulace genové exprese účinky léků   MeSH
• trialkylcínové sloučeniny farmakokinetika   toxicita   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Čína MeSH
• Názvy látek
• antioxidancia MeSH
• biologické markery MeSH
• chemické látky znečišťující vodu MeSH
• endokrinní disruptory MeSH
• malondialdehyd MeSH
• trialkylcínové sloučeniny MeSH
• tributyltin MeSH Prohlížeč

The aim of the present study was to investigate the effect of Tributyltin (TBT) on brain function and neurotoxicity of freshwater teleost. The effects of long-term exposure to TBT on antioxidant related indices (MDA, malondialdehyde; SOD, superoxide dismutase; CAT, catalase; GR, glutathione reductase; GPx, glutathione peroxidase), Na+-K+-ATPase and neurological parameters (AChE, acetylcholinesterase; MAO, monoamine oxidase; NO, nitric oxide) in the brain of common carp were evaluated. Fish were exposed to sublethal concentrations of TBT (75 ng/L, 0.75 μg/L and 7.5 μg/L) for 15, 30, and 60 days. Based on the results, a low level and short-term TBT-induced stress could not induce the notable responses of the fish brain, but long-term exposure (more than 15 days) to TBT could lead to obvious physiological-biochemical responses (based on the measured parameters). The results also strongly indicated that neurotoxicity of TBT to fish. Thus, the measured physiological responses in fish brain could provide useful information to better understand the mechanisms of TBT-induced bio-toxicity.

• MeSH
• antioxidancia metabolismus   MeSH
• chemické látky znečišťující vodu toxicita   MeSH
• kapři MeSH
• mozek účinky léků   MeSH
• oxidační stres MeSH
• trialkylcínové sloučeniny toxicita   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antioxidancia MeSH
• chemické látky znečišťující vodu MeSH
• trialkylcínové sloučeniny MeSH
• tributyltin MeSH Prohlížeč

Tributyltin (TBT) is a ubiquitous contaminant in aquatic environment, but the detailed mechanisms underlying the toxicity of TBT have not been fully understood. In this study, the effects of TBT on behavior, energy metabolism and endoplasmic reticulum (ER) stress were investigated by using Chinese rare minnow larvae. Fish larvae were exposed at sublethal concentrations of TBT (100, 400 and 800 ng/L) for 7 days. Compared with the control, energy metabolic parameters (RNA/DNA ratio, Na(+)-K(+)-ATPase) were significantly inhibited in fish exposed at highest concentration (800 ng/L), as well as abnormal behaviors observed. Moreover, we found that the PERK (PKR-like ER kinase)-eIF2α (eukaryotic translation initiation factor 2α) pathway, as the main branch was activated by TBT exposure in fish larvae. In short, TBT-induced physiological, biochemical and molecular responses in fish larvae were reflected in parameters measured in this study, which suggest that these biomarkers could be used as potential indicators for monitoring organotin compounds present in aquatic environment.

• Klíčová slova
• Behavior, Endoplasmic reticulum stress, Energy metabolism, Fish, Tributyltin,
• MeSH
• chování zvířat účinky léků   MeSH
• Cyprinidae růst a vývoj   MeSH
• DNA vazebné proteiny genetika   metabolismus   MeSH
• endoribonukleasy genetika   metabolismus   MeSH
• energetický metabolismus účinky léků   MeSH
• kinasa eIF-2 genetika   metabolismus   MeSH
• larva účinky léků   metabolismus   MeSH
• stres endoplazmatického retikula účinky léků   MeSH
• transkripční faktor ATF4 genetika   metabolismus   MeSH
• transkripční faktory RFX MeSH
• transkripční faktory genetika   metabolismus   MeSH
• trialkylcínové sloučeniny toxicita   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Čína MeSH
• Názvy látek
• DNA vazebné proteiny MeSH
• endoribonukleasy MeSH
• kinasa eIF-2 MeSH
• transkripční faktor ATF4 MeSH
• transkripční faktory RFX MeSH
• transkripční faktory MeSH
• trialkylcínové sloučeniny MeSH
• tributyltin MeSH Prohlížeč