This work describes the preparation of highly homogeneous thermoplastic starches (TPS's) with the addition of 0, 5, or 10 wt.% of maltodextrin (MD) and 0 or 3 wt.% of TiO2 nanoparticles. The TPS preparation was based on a two-step preparation protocol, which consisted in solution casting (SC) followed by melt mixing (MM). Rheology measurements at the typical starch processing temperature (120 °C) demonstrated that maltodextrin acted as a lubricating agent, which decreased the viscosity of the system. Consequently, the in situ measurement during the MM confirmed that the torque moments and real processing temperatures of all TPS/MD systems decreased in comparison with the pure TPS. The detailed characterization of morphology, thermomechanical properties, and local mechanical properties revealed that the viscosity decrease was accompanied by a slight decrease in the system homogeneity. The changes in the real processing temperatures might be quite moderate (ca 2-3 °C), but maltodextrin is a cheap and easy-to-add modifier, and the milder processing conditions are advantageous for both technical applications (energy savings) and biomedical applications (beneficial for temperature-sensitive additives, such as antibiotics).
- Klíčová slova
- lower processing temperature, lower viscosity, maltodextrin modification, thermoplastic starch,
- Publikační typ
- časopisecké články MeSH
The control of the surface properties is an important issue for applicability of polymer membranes interacting with cells. In this work, the influence of surface roughness and stiffness of two polymer membranes on viability and mechanical properties of keratinocytes was studied. Terpolimer polyglicolide, polycaprolactone and polylactide, (PGA-PCL-PLA) and copolymer polycaprolactone, polyglicolide (PGA-PCL) substrates were used for membranes fabrication. Surface modification - the hydrolysis of the obtained membranes was carried out. The analysis of membranes' surface properties revealed that RMS surface roughness and roughness factor of PGA-PCL-PLA membrane decreased after hydrolysis while its stiffness increased. In contrast, the PGA-PCL membrane stiffness was only slightly affected by NaOH treatment. Immortalized human keratinocytes (HaCaT) were grown under standard conditions on the surface of the studied membranes and characterized by means of atomic force microscopy and fluorescence microcopy. The results showed the substrate-dependent effect on cells' properties.
- Klíčová slova
- Atomic force microscopy, HaCaT cells viability, Mechanical properties, Polyester membranes, Surface properties,
- MeSH
- buněčné linie MeSH
- fluorescenční mikroskopie MeSH
- keratinocyty účinky léků fyziologie MeSH
- lidé MeSH
- membrány chemie MeSH
- mikroskopie atomárních sil MeSH
- polymery chemická syntéza toxicita MeSH
- povrchové vlastnosti * MeSH
- testování materiálů * MeSH
- viabilita buněk MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- polymery MeSH
A deep understanding of the interaction between cancerous cells and surfaces is particularly important for the design of lab-on-chip devices involving the use of polydimethylsiloxane (PDMS). In our studies, the effect of PDMS substrate stiffness on mechanical properties of cancerous cells was investigated in conditions where the PDMS substrate is not covered with any of extracellular matrix proteins. Two human prostate cancer (Du145 and PC-3) and two melanoma (WM115 and WM266-4) cell lines were cultured on two groups of PDMS substrates that were characterized by distinct stiffness, i.e. 0.75 ± 0.06 MPa and 2.92 ± 0.12 MPa. The results showed the strong effect on cellular behavior and morphology. The detailed analysis of chemical and physical properties of substrates revealed that cellular behavior occurs only due to substrate elasticity.
- Klíčová slova
- Cancer cell growth profile, PDMS stiffness, Poly(dimethyl)siloxane (PDMS) surface, Prostate and melanoma,
- MeSH
- biomechanika MeSH
- dimethylpolysiloxany chemie farmakologie MeSH
- fibrinogen chemie MeSH
- lidé MeSH
- mechanické jevy * MeSH
- melanom patologie MeSH
- mikročipové analytické postupy MeSH
- nádorové buněčné linie MeSH
- nádory prostaty patologie MeSH
- povrchové vlastnosti MeSH
- proliferace buněk účinky léků MeSH
- pružnost MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- baysilon MeSH Prohlížeč
- dimethylpolysiloxany MeSH
- fibrinogen MeSH
