Aquatic bacterial rhodopsin proton pumps harvest light energy for photoheterotrophic growth and are known to contain hydroxylated carotenoids that expand the wavelengths of light utilized, but these have not been characterized in marine archaea. Here, by combining a marine chromophore extract with purified archaeal rhodopsins identified in marine metagenomes, we show light energy transfer from diverse hydroxylated carotenoids to heimdallarchaeial rhodopsins (HeimdallRs) from uncultured marine planktonic members of 'Candidatus Kariarchaeaceae' ('Candidatus Asgardarchaeota'). These light-harvesting antennas absorb in the blue-light range and transfer energy to the green-light-absorbing retinal chromophore within HeimdallRs, enabling the use of light that is otherwise unavailable to the rhodopsin. Furthermore, we show elevated proton pumping by the antennas in HeimdallRs under white-light illumination, which better simulates the light conditions encountered by these archaea in their natural habitats. Our results indicate that light-harvesting antennas in microbial rhodopsins exist in families beyond xanthorhodopsins and proteorhodopsins and are present in both marine bacteria and archaea.

• MeSH
• Archaea * metabolismus   genetika   chemie   MeSH
• archeální proteiny * metabolismus   chemie   genetika   MeSH
• fylogeneze MeSH
• karotenoidy metabolismus   chemie   MeSH
• metagenom MeSH
• mořská voda mikrobiologie   MeSH
• přenos energie MeSH
• rhodopsiny mikrobiální * chemie   metabolismus   MeSH
• rodopsin * chemie   metabolismus   MeSH
• světlo MeSH
• světlosběrné proteinové komplexy * chemie   metabolismus   MeSH
• vodní organismy metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• archeální proteiny * MeSH
• karotenoidy MeSH
• rhodopsiny mikrobiální * MeSH
• rodopsin * MeSH
• světlosběrné proteinové komplexy * MeSH

Female insects can enter reproductive diapause, a state of suspended egg development, to conserve energy under adverse environments. In many insects, including the fruit fly, Drosophila melanogaster, reproductive diapause, also frequently called reproductive dormancy, is induced under low-temperature and short-day conditions by the downregulation of juvenile hormone (JH) biosynthesis in the corpus allatum (CA). In this study, we demonstrate that neuropeptide Diuretic hormone 31 (DH31) produced by brain neurons that project into the CA plays an essential role in regulating reproductive dormancy by suppressing JH biosynthesis in adult D. melanogaster. The CA expresses the gene encoding the DH31 receptor, which is required for DH31-triggered elevation of intracellular cAMP in the CA. Knocking down Dh31 in these CA-projecting neurons or DH31 receptor in the CA suppresses the decrease of JH titer, normally observed under dormancy-inducing conditions, leading to abnormal yolk accumulation in the ovaries. Our findings provide the first molecular genetic evidence demonstrating that CA-projecting peptidergic neurons play an essential role in regulating reproductive dormancy by suppressing JH biosynthesis.

• Klíčová slova
• Drosophila, Corpus allatum, Diapause, Diuretic hormone 31, Juvenile hormone, Reproductive dormancy,
• MeSH
• corpora allata MeSH
• Drosophila melanogaster * genetika   fyziologie   MeSH
• hmyzí hormony * genetika   fyziologie   MeSH
• juvenilní hormony MeSH
• neurony MeSH
• proteiny Drosophily genetika   fyziologie   MeSH
• rozmnožování MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• Dh31 protein, Drosophila MeSH Prohlížeč
• hmyzí hormony * MeSH
• juvenilní hormony MeSH
• proteiny Drosophily MeSH