Singlet oxygen produced from triplet excited chlorophylls in photosynthesis is a signal molecule that can induce programmed cell death (PCD) through the action of the OXIDATIVE STRESS INDUCIBLE 1 (OXI1) kinase. Here, we identify two negative regulators of light-induced PCD that modulate OXI1 expression: DAD1 and DAD2, homologs of the human antiapoptotic protein DEFENDER AGAINST CELL DEATH. Overexpressing OXI1 in Arabidopsis (Arabidopsis thaliana) increased plant sensitivity to high light and induced early senescence of mature leaves. Both phenomena rely on a marked accumulation of jasmonate and salicylate. DAD1 or DAD2 overexpression decreased OXI1 expression, jasmonate levels, and sensitivity to photooxidative stress. Knock-out mutants of DAD1 or DAD2 exhibited the opposite responses. Exogenous applications of jasmonate upregulated salicylate biosynthesis genes and caused leaf damage in wild-type plants but not in the salicylate biosynthesis mutant Salicylic acid induction-deficient2, indicating that salicylate plays a crucial role in PCD downstream of jasmonate. Treating plants with salicylate upregulated the DAD genes and downregulated OXI1 We conclude that OXI1 and DAD are antagonistic regulators of cell death through modulating jasmonate and salicylate levels. High light-induced PCD thus results from a tight control of the relative activities of these regulating proteins, with DAD exerting a negative feedback control on OXI1 expression.
- MeSH
- apoptóza genetika účinky záření MeSH
- Arabidopsis cytologie genetika metabolismus MeSH
- biosyntetické dráhy účinky léků genetika účinky záření MeSH
- cyklopentany metabolismus farmakologie MeSH
- fosfolipasy A1 genetika metabolismus MeSH
- kyselina salicylová metabolismus farmakologie MeSH
- listy rostlin cytologie genetika metabolismus MeSH
- mutace MeSH
- oxylipiny metabolismus farmakologie MeSH
- protein-serin-threoninkinasy genetika metabolismus MeSH
- proteiny huseníčku genetika metabolismus MeSH
- regulace genové exprese u rostlin účinky léků účinky záření MeSH
- regulátory růstu rostlin metabolismus farmakologie MeSH
- singletový kyslík metabolismus MeSH
- stanovení celkové genové exprese metody MeSH
- světlo MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cyklopentany MeSH
- DAD1 protein, Arabidopsis MeSH Prohlížeč
- fosfolipasy A1 MeSH
- jasmonic acid MeSH Prohlížeč
- kyselina salicylová MeSH
- OXI1 protein, Arabidopsis MeSH Prohlížeč
- oxylipiny MeSH
- protein-serin-threoninkinasy MeSH
- proteiny huseníčku MeSH
- regulátory růstu rostlin MeSH
- singletový kyslík MeSH
