Three fluorescent probes, tetramethyl rhodamine ethyl ester (TMRE), 3,3'-dipropylthiacarbocyanine iodide (diS-C3(3)) and 3,3'-dipropyloxacarbocyanine iodide (diO-C3(3)), were tested for their suitability as fluorescent indicators of membrane potential in Saccharomyces cerevisiae in studies performed by flow cytometry. For all these dyes the intensity of fluorescence of stained cells increased with probe concentration in the range of 60-3000 nmol/L. The optimum staining period was 15-20 min for diS-C3(3). Depolarization of cells by increased extracellular potassium level and by valinomycin elicited with all probes a drop in fluorescence intensity. In some yeast batches this depolarization was accompanied by a separation of subpopulations with different fluorescence properties.

• MeSH
• artefakty MeSH
• benzothiazoly MeSH
• časové faktory MeSH
• fluorescence MeSH
• fluorescenční barviva * MeSH
• fluorometrie * MeSH
• indikátory a reagencie MeSH
• karbocyaniny * MeSH
• membránové potenciály * MeSH
• organokovové sloučeniny MeSH
• průtoková cytometrie metody   MeSH
• pyridinové sloučeniny analýza   metabolismus   MeSH
• radiační rozptyl MeSH
• Saccharomyces cerevisiae fyziologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• 1-(3-sulfonatopropyl)-4-(beta)(2-(di-n-butylamino)-6-naphthylvinyl)pyridinium betaine MeSH Prohlížeč
• 3,3'-dipropyl-2,2'-thiadicarbocyanine MeSH Prohlížeč
• 3,3'-dipropyloxadicarbocyanine MeSH Prohlížeč
• 3,3'-dipropylthiacarbocyanine MeSH Prohlížeč
• benzothiazoly MeSH
• fluorescenční barviva * MeSH
• indikátory a reagencie MeSH
• karbocyaniny * MeSH
• organokovové sloučeniny MeSH
• pyridinové sloučeniny MeSH
• tetramethyl rhodamine ethyl ester MeSH Prohlížeč