Three fluorescent probes for the flow-cytometric assessment of membrane potential in Saccharomyces cerevisiae
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
9449773
DOI
10.1007/bf02814623
Knihovny.cz E-zdroje
- MeSH
- artefakty MeSH
- benzothiazoly MeSH
- časové faktory MeSH
- fluorescence MeSH
- fluorescenční barviva * MeSH
- fluorometrie * MeSH
- indikátory a reagencie MeSH
- karbocyaniny * MeSH
- membránové potenciály * MeSH
- organokovové sloučeniny MeSH
- průtoková cytometrie metody MeSH
- pyridinové sloučeniny analýza metabolismus MeSH
- radiační rozptyl MeSH
- Saccharomyces cerevisiae fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 1-(3-sulfonatopropyl)-4-(beta)(2-(di-n-butylamino)-6-naphthylvinyl)pyridinium betaine MeSH Prohlížeč
- 3,3'-dipropyl-2,2'-thiadicarbocyanine MeSH Prohlížeč
- 3,3'-dipropyloxadicarbocyanine MeSH Prohlížeč
- 3,3'-dipropylthiacarbocyanine MeSH Prohlížeč
- benzothiazoly MeSH
- fluorescenční barviva * MeSH
- indikátory a reagencie MeSH
- karbocyaniny * MeSH
- organokovové sloučeniny MeSH
- pyridinové sloučeniny MeSH
- tetramethyl rhodamine ethyl ester MeSH Prohlížeč
Three fluorescent probes, tetramethyl rhodamine ethyl ester (TMRE), 3,3'-dipropylthiacarbocyanine iodide (diS-C3(3)) and 3,3'-dipropyloxacarbocyanine iodide (diO-C3(3)), were tested for their suitability as fluorescent indicators of membrane potential in Saccharomyces cerevisiae in studies performed by flow cytometry. For all these dyes the intensity of fluorescence of stained cells increased with probe concentration in the range of 60-3000 nmol/L. The optimum staining period was 15-20 min for diS-C3(3). Depolarization of cells by increased extracellular potassium level and by valinomycin elicited with all probes a drop in fluorescence intensity. In some yeast batches this depolarization was accompanied by a separation of subpopulations with different fluorescence properties.
Zobrazit více v PubMed
J Fluoresc. 1993 Sep;3(3):157-9 PubMed
Biochim Biophys Acta. 1990 Mar 15;1016(1):1-28 PubMed
Biochim Biophys Acta. 1994 Dec 30;1196(2):181-90 PubMed
Biochim Biophys Acta. 1988 Dec 7;936(3):421-8 PubMed
Yeast. 1993 Nov;9(11):1207-11 PubMed
Experientia. 1995 Jan 15;51(1):26-8 PubMed
J Photochem Photobiol B. 1996 Apr;33(2):101-24 PubMed
