We propose the first comprehensive in-depth study monitoring horses in the Czech Republic. We scanned 9,289 animals from 44 populations for 17 equine STRs. Other equids analysed involved Equus przewalskii and Equus asinus. The total of 228 different alleles were detected, with the mean number of 13.4 per locus. The highest allelic richness (AR) was found in the Welsh Part Bred (6.01), followed by the Camargue (5.93) and Czech Sport Pony (5.91), whereas the Friesian exhibited the lowest AR (3.06). Interpopulation differences explained approximately nine per cent of the total genetic diversity. Reynold's genetic distance ranged from 0.003 between the Czech Warmblood and the Slovak Warmblood to 0.404 between the Friesian and donkeys. Close genetic proximity between the Silesian Noriker and Noriker was revealed. The Moravian Warmblood was better differentiated and more distant from the Czech Warmblood than the Kinsky Horse and retained the original genes of the old Austro-Hungarian tribes. A high gene flow level and a lack of genetic structure were found in the seven studied populations. Despite the historical bottlenecks and previous inbreeding, the Czech-Moravian Belgian Horse, Hucul, Old Kladruber Horse and Silesian Noriker did not suffer a serious loss of genetic diversity due to genetic drift/low effective population size. A NeighborNet dendrogram revealed breeds not classified in their groups according to the nomenclature (the Friesian, Hafling and Merens).
- Klíčová slova
- autochthonous, genetic diversity, horse, phylogenetic relationships,
- MeSH
- chov MeSH
- genetická variace MeSH
- genotyp MeSH
- koně genetika MeSH
- shluková analýza MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
Individual variation in immune responses to herpesviruses was observed in various species. Here, associations between polymorphic molecular markers and life-long anti-EHV-1/4 antibody immune responses were analyzed in a model EHV-infected population of the Old Kladruber horses. Two-dimensional analysis including overall mean titers and titer dynamics expressed by differences between spring and autumn titers allowed identification of low-responders. 50 randomly selected microsatellites and nine single nucleotide polymorphisms in nine immunity-related candidate genes were genotyped. Due to differences (p<0.001) in antibody titers between two color varieties of Old Kladruber horses, separate association studies were performed in the two sub-populations by using the Fisher's exact test. In black horses, the interleukin 4 receptor and MxA protein coding genes, and the microsatellite TKY325 were associated with the responder status. In the grey population, the microsatellite TKY343 showed significant association with anti-EHV antibody responsiveness after Bonferroni corrections.
- MeSH
- fenotyp MeSH
- genotyp MeSH
- herpesvirus 1 koní genetika imunologie MeSH
- herpetické infekce genetika imunologie veterinární virologie MeSH
- jednonukleotidový polymorfismus genetika MeSH
- koně MeSH
- mikrosatelitní repetice MeSH
- nemoci koní genetika imunologie virologie MeSH
- neparametrická statistika MeSH
- polymerázová řetězová reakce veterinární MeSH
- protilátky virové krev MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- protilátky virové MeSH
The genotypes of growth hormone gene polymorphisms (GH-DdeI, GH-MspI, GH-HaeII, GH-ApaI, GH-CfoI) were determined in 78 pigs [Czech Large White sires (CLWsire line) x Polish Large White (PLW) sows, Polish Large White sires x Polish Large White sows], by the PCR-RFLP method. Preliminary studies found only GH DdeI polymorphism to be associated with performance traits. The associations of this polymorphism with growth and carcass traits were investigated. The linear model included the effects of candidate genes, genetic groups, sex and linear covariables of age at slaughter and body weight at weaning. The DdeI polymorphism of the GH gene showed associations with carcass length (P
- Publikační typ
- časopisecké články MeSH
- MeSH
- frekvence genu MeSH
- iontové kanály MeSH
- mapování chromozomů veterinární MeSH
- mitochondriální proteiny MeSH
- polymerázová řetězová reakce veterinární MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- prasata genetika MeSH
- restrikční endonukleasy typu II metabolismus MeSH
- transportní proteiny genetika MeSH
- uncoupling protein 3 MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- CYCGRG-specific type II deoxyribonucleases MeSH Prohlížeč
- iontové kanály MeSH
- mitochondriální proteiny MeSH
- restrikční endonukleasy typu II MeSH
- transportní proteiny MeSH
- uncoupling protein 3 MeSH
- MeSH
- insulinu podobný růstový faktor II genetika MeSH
- introny * MeSH
- polymerázová řetězová reakce veterinární MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- polymorfismus genetický MeSH
- prasata genetika MeSH
- restrikční endonukleasy typu II metabolismus MeSH
- rodokmen MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- CCSGG-specific type II deoxyribonucleases MeSH Prohlížeč
- insulinu podobný růstový faktor II MeSH
- restrikční endonukleasy typu II MeSH
