A decrease in the abundance and biodiversity of intestinal bacteria within the Firmicutes phylum has been associated with inflammatory bowel disease (IBD). In particular, the anti-inflammatory bacterium Faecalibacterium prausnitzii, member of the Firmicutes phylum and one of the most abundant species in healthy human colon, is underrepresented in the microbiota of IBD patients. The aim of this study was to investigate the immunomodulatory properties of F. prausnitzii strain A2-165, the biofilm forming strain HTF-F and the extracellular polymeric matrix (EPM) isolated from strain HTF-F. For this purpose, the immunomodulatory properties of the F. prausnitzii strains and the EPM were studied in vitro using human monocyte-derived dendritic cells. Then, the capacity of the F. prausnitzii strains and the EPM of HTF-F to suppress inflammation was assessed in vivo in the mouse dextran sodium sulphate (DSS) colitis model. The F. prausnitzii strains and the EPM had anti-inflammatory effects on the clinical parameters measured in the DSS model but with different efficacy. The immunomodulatory effects of the EPM were mediated through the TLR2-dependent modulation of IL-12 and IL-10 cytokine production in antigen presenting cells, suggesting that it contributes to the anti-inflammatory potency of F. prausnitzii HTF-F. The results show that F. prausnitzii HTF-F and its EPM may have a therapeutic use in IBD.
- MeSH
- antigeny povrchové metabolismus MeSH
- cytokiny genetika metabolismus MeSH
- dendritické buňky imunologie metabolismus MeSH
- extracelulární matrix metabolismus MeSH
- fenotyp MeSH
- forkhead transkripční faktory genetika metabolismus MeSH
- genetická transkripce MeSH
- idiopatické střevní záněty etiologie metabolismus patologie MeSH
- kolitida chemicky indukované genetika imunologie metabolismus mikrobiologie MeSH
- lymfatické uzliny imunologie metabolismus MeSH
- mediátory zánětu metabolismus MeSH
- modely nemocí na zvířatech MeSH
- myši MeSH
- Ruminococcus metabolismus ultrastruktura MeSH
- síran dextranu škodlivé účinky MeSH
- slezina imunologie metabolismus MeSH
- střevní sliznice metabolismus mikrobiologie patologie MeSH
- toll-like receptor 2 genetika metabolismus MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antigeny povrchové MeSH
- cytokiny MeSH
- forkhead transkripční faktory MeSH
- FOXP3 protein, human MeSH Prohlížeč
- mediátory zánětu MeSH
- síran dextranu MeSH
- toll-like receptor 2 MeSH
