A two-dimensional electrophoretic method was used to show that protein degradation occurs immediately after the end of exponential growth but that its occurrence is masked in the usual assay methods for a 2-h period and that degradation is apparently nonselective with respect to protein molar mass or charge. The results suggest that considerable reutilization of internal amino acids may occur during sporulation regardless of the size of the external chase. Finally, the levels of intracellular proteinase activities present even at the end of exponential phase growth, as measured in vitro, are sufficient to account for the maximum rates of protein degradation observed in vivo.

• MeSH
• Bacillus subtilis enzymologie   metabolismus   fyziologie   MeSH
• bakteriální proteiny analýza   biosyntéza   metabolismus   MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• endopeptidasy metabolismus   MeSH
• isoelektrická fokusace MeSH
• koncentrace vodíkových iontů MeSH
• leucin analýza   metabolismus   MeSH
• matematika MeSH
• molekulová hmotnost MeSH
• spory bakteriální MeSH
• Publikační typ
• časopisecké články MeSH
• Research Support, U.S. Gov't, P.H.S. MeSH
• Názvy látek
• bakteriální proteiny MeSH
• endopeptidasy MeSH
• leucin MeSH