The aim of the study was to broadly determine the biological activities of purple potato ethanolic extract of the Blue Congo variety (BCE). The antioxidant activity of BCE was determined in relation to liposome membranes, and peroxidation was induced by UVB and AAPH. To clarify the antioxidant activity of BCE, we investigated its interactions with hydrophilic and hydrophobic regions of a membrane using fluorimetric and FTIR methods. Next, we investigated the cytotoxicity and pro-apoptotic activities of BCE in two human colon cancer cell lines (HT-29 and Caco-2) and in normal cells (IPEC-J2). In addition, the ability to inhibit enzymes that are involved in pro-inflammatory reactions was examined. Furthermore, BCE interactions with serum albumin and plasmid DNA were investigated using steady state fluorescence spectroscopy and a single molecule fluorescence technique (TCSPC-FCS). We proved that BCE effectively protects lipid membranes against the process of peroxidation and successfully inhibits the cyclooxygenase and lipoxygenase enzymes. Furthermore, it interacts with the hydrophilic and hydrophobic parts of lipid membranes as well as with albumin and plasmid DNA. It was observed that BCE is more cytotoxic against colon cancer cell lines than normal IPEC-J2 cells; it also induces apoptosis in cancer cell lines, but does not induce cell death in normal cells.

• MeSH
• albuminy MeSH
• antioxidancia chemie   farmakologie   MeSH
• antitumorózní látky fytogenní chemie   farmakologie   MeSH
• inhibitory cyklooxygenasy chemie   farmakologie   MeSH
• inhibitory lipoxygenas chemie   farmakologie   MeSH
• lidé MeSH
• lipidy chemie   MeSH
• liposomy MeSH
• nádorové buněčné linie MeSH
• plazmidy MeSH
• reaktivní formy kyslíku MeSH
• rostlinné extrakty chemie   farmakologie   MeSH
• sérový albumin chemie   metabolismus   MeSH
• Solanum tuberosum chemie   MeSH
• vazba proteinů MeSH
• viabilita buněk účinky léků   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• albuminy MeSH
• antioxidancia MeSH
• antitumorózní látky fytogenní MeSH
• inhibitory cyklooxygenasy MeSH
• inhibitory lipoxygenas MeSH
• lipidy MeSH
• liposomy MeSH
• reaktivní formy kyslíku MeSH
• rostlinné extrakty MeSH
• sérový albumin MeSH