This study involved creating oligomeric conjugates of 3-hydroxy fatty acids and diclofenac, named Dic-oligo(3HAs). Advanced NMR techniques confirmed no free diclofenac in the mix. We tested diclofenac release under conditions resembling healthy and chronic wound skin. These oligomers were used to make P(3HO) blends, forming patches for drug delivery. Their preparation used the solvent casting/porogen leaching (SCPL) method. The patches' properties like porosity, roughness, and wettability were thoroughly analysed. Antimicrobial assays showed that Dic-oligo(3HAs) exhibited antimicrobial activity against reference (S. aureus, S. epidermis, S. faecalis) and clinical (Staphylococcus spp.) strains. Human keratinocytes (HaCaT) cell line tests, as per ISO 10993-5, showed no toxicity. A clear link between material roughness and HaCaT cell adhesion was found. Deep cell infiltration was verified using DAPI and phalloidin staining, observed under confocal microscopy. SEM also confirmed HaCaT cell growth on these scaffolds. The strong adhesion and proliferation of HaCaT cells on these materials indicate their potential as wound dressing layers. Additionally, the successful diclofenac release tests point to their applicability in treating both normal and chronic wounds.
- Klíčová slova
- Artificial skin substitute, bioresorbable, porous layer of dressing material, Poly(3-hydroxyoctanoate), Polyhydroxyalkanoates, Targeted delivery of diclofenac,
- MeSH
- biokompatibilní materiály chemie farmakologie MeSH
- buněčné linie keratinocytů HaCaT MeSH
- buněčné linie MeSH
- chemické jevy MeSH
- diklofenak * farmakologie chemie MeSH
- hojení ran účinky léků MeSH
- keratinocyty účinky léků cytologie MeSH
- kůže * účinky léků MeSH
- lidé MeSH
- polymery chemie MeSH
- poréznost MeSH
- proliferace buněk účinky léků MeSH
- regenerace účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
A novel, eco-friendly, and biocompatible method was applied to form silver nanoparticles (AgNPs) in great water dock (Lapathi radix) (KB) and pu-erh (Camellia sinensis) (PE) extracts. The surface plasma resonance peak of green synthesized AgNPs at 451.8 nm for AgNPs+KB and 440.8 nm for AgNPs+PE was observed via spectral analysis of UV absorbance. In this study, double-layered biopolymer films (FUR/CHIT+HGEL) with AgNPs incorporated in KB solution (AgNPs+KB) and AgNPs in PE solution (AgNPs+PE), were successfully prepared using the casting method. The SEM, XRD, zeta potential and size analyses confirmed the presence of AgNP in the films. The addition of AgNPs in plant extracts improved antimicrobial and antioxidant activity and thermal stability, whereas WVTR experienced a decrease. The nanocomposite films' orange-brown colour may aid in the protection of food products against UV rays. The composite films demonstrated antibacterial activity against food-borne pathogens and may offer potential in food packaging applications.
- Klíčová slova
- chitosan, double-layered films, furcellaran, gelatin hydrolysate, silver nanoparticles,
- Publikační typ
- časopisecké články MeSH
Graphene oxide (GO) and multi-walled carbon nanotubes (MWCNTs) were added to furcellaran films (FUR). Silver nanoparticles (AgNPs) were prepared by reducing AgNO3 using a FUR matrix as the stabilising agent. The structure and surface morphology of nanocomposite films were obtained using FTIR, SEM and XRD. The molecular weights of furcellaran chains were estimated using HPSEC-MALLS-RI. Characterisation of the films was undertaken to analyse their physical, mechanical and structural properties. SEM analysis revealed that GO, MWCNTs and AgNPs were evenly distributed throughout the FUR surface. FUR + AgNP films showed antimicrobial activity against bacteria and fungi. P. aeruginosa, E. faecalis and S. aureus were the most affected with effective growth inhibition using the disc diffusion method. In the study, the effect of nanofillers on the structural, thermal, mechanical and antimicrobial properties of furcellaran films as potential materials for food packaging is presented.
- Klíčová slova
- Furcellaran, Graphene oxide, Multi-walled carbon nanotubes, Nanocomposite films, Nanofillers, Silver nanoparticles,
- MeSH
- algináty chemie MeSH
- antibakteriální látky chemie farmakologie MeSH
- Bacteria účinky léků MeSH
- biopolymery chemie MeSH
- grafit chemie MeSH
- kovové nanočástice chemie MeSH
- nanokompozity chemie MeSH
- nanotrubičky uhlíkové chemie MeSH
- rostlinné gumy chemie MeSH
- stříbro chemie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- algináty MeSH
- antibakteriální látky MeSH
- biopolymery MeSH
- furcellaran MeSH Prohlížeč
- grafit MeSH
- graphene oxide MeSH Prohlížeč
- nanotrubičky uhlíkové MeSH
- rostlinné gumy MeSH
- stříbro MeSH
