Physicochemical, structural and biological characterisation of poly(3-hydroxyoctanoate) supplemented with diclofenac acid conjugates - Harnessing the potential in the construction of materials for skin regeneration processes
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
38614181
DOI
10.1016/j.ijbiomac.2024.131476
PII: S0141-8130(24)02281-5
Knihovny.cz E-zdroje
- Klíčová slova
- Artificial skin substitute, bioresorbable, porous layer of dressing material, Poly(3-hydroxyoctanoate), Polyhydroxyalkanoates, Targeted delivery of diclofenac,
- MeSH
- biokompatibilní materiály chemie farmakologie MeSH
- buněčné linie keratinocytů HaCaT MeSH
- buněčné linie MeSH
- chemické jevy MeSH
- diklofenak * farmakologie chemie MeSH
- hojení ran účinky léků MeSH
- keratinocyty účinky léků cytologie MeSH
- kůže * účinky léků MeSH
- lidé MeSH
- polymery chemie MeSH
- poréznost MeSH
- proliferace buněk účinky léků MeSH
- regenerace účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
This study involved creating oligomeric conjugates of 3-hydroxy fatty acids and diclofenac, named Dic-oligo(3HAs). Advanced NMR techniques confirmed no free diclofenac in the mix. We tested diclofenac release under conditions resembling healthy and chronic wound skin. These oligomers were used to make P(3HO) blends, forming patches for drug delivery. Their preparation used the solvent casting/porogen leaching (SCPL) method. The patches' properties like porosity, roughness, and wettability were thoroughly analysed. Antimicrobial assays showed that Dic-oligo(3HAs) exhibited antimicrobial activity against reference (S. aureus, S. epidermis, S. faecalis) and clinical (Staphylococcus spp.) strains. Human keratinocytes (HaCaT) cell line tests, as per ISO 10993-5, showed no toxicity. A clear link between material roughness and HaCaT cell adhesion was found. Deep cell infiltration was verified using DAPI and phalloidin staining, observed under confocal microscopy. SEM also confirmed HaCaT cell growth on these scaffolds. The strong adhesion and proliferation of HaCaT cells on these materials indicate their potential as wound dressing layers. Additionally, the successful diclofenac release tests point to their applicability in treating both normal and chronic wounds.
Citace poskytuje Crossref.org
