A novel porcine gene, alpha-1-antichymotrypsin 2 (SERPINA3-2), a member of the serpin superfamily, was isolated from a porcine genomic library and sequenced. The genomic organization of the approximately 9.0 kb gene was determined on the basis of the porcine liver cDNA of SERPINA3-1 and SERPINA3-2, and comprises five exons and four introns. The coding sequence of SERPINA3-2 shares 86% identity with the paralogue, SERPINA3-1. Porcine SERPINA3-2 was found to be an orthologue of human SERPINA3 (71% identity of the coding sequences) and both genes have a similar genomic organization. Polymorphisms were found in intron 4 of the porcine gene using polymerase chain reaction-restriction fragment length polymorphism. The gene was mapped by linkage analysis and radiation hybrid mapping to the distal end of chromosome 7q, to the gene cluster of the protease inhibitors including PI1 (SERPINA1), PI2, PI3, PI4 (apparently paralogues of SERPINA3), and PO1A and PO1B. SERPINA3-2 is the first porcine serpin gene whose genomic organization has been determined.

• MeSH
• alfa-1-antichymotrypsin genetika   MeSH
• DNA chemie   genetika   MeSH
• exony MeSH
• geny genetika   MeSH
• introny MeSH
• klonování DNA MeSH
• komplementární DNA chemie   genetika   MeSH
• mapování chromozomů MeSH
• mapování pomocí radiačních hybridů MeSH
• molekulární sekvence - údaje MeSH
• polymorfismus genetický MeSH
• prasata genetika   MeSH
• sekvence aminokyselin MeSH
• sekvence nukleotidů MeSH
• sekvenční analýza DNA MeSH
• sekvenční homologie aminokyselin MeSH
• sekvenční homologie nukleových kyselin MeSH
• serpiny genetika   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• alfa-1-antichymotrypsin MeSH
• DNA MeSH
• komplementární DNA MeSH
• serpiny MeSH

• MeSH
• alfa-1-antichymotrypsin genetika   MeSH
• DNA primery MeSH
• mapování chromozomů * MeSH
• molekulární sekvence - údaje MeSH
• prasata genetika   MeSH
• sekvence nukleotidů MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• alfa-1-antichymotrypsin MeSH
• DNA primery MeSH

Three related alpha-protease inhibitors, PI2 I, PI3 C and PI4 C2, of blood serum of the pig (Sus scrofa) were isolated. PI2 I inhibited both trypsin and chymotrypsin; PI3 C and PI4 C2 strongly inhibited chymotrypsin, but did not significantly inhibit trypsin. By using SDS-PAGE, the three proteins were found to be composed of single polypeptide chains, and molecular weights were 63,000 for PI2 I, 58,000 for PI3 C and 64,000 for PI4 C2. All three proteins were shown to be glycoproteins. In PI3 C, eight sialic acid residues were found, and in PI4 C2 (similarly as in PI2 F) 10-11 residues were found. Amino acid composition as well as N-terminal sequences of the three proteins were very similar, indicating close homology. Comparison of these partial amino acid sequences with the cDNA-deduced amino acid sequence of pig alpha-antichymotrypsin (AACT; Buchman, 1989, GenBank, Accession No. M29508) revealed great similarities, the sequence of PI2 I being virtually identical with the pig AACT. On the basis of all available results, PI2 is proposed to be pig AACT, an orthologue of human AACT.

• MeSH
• alfa-1-antichymotrypsin krev   chemie   MeSH
• aminokyseliny analýza   MeSH
• chymotrypsin antagonisté a inhibitory   MeSH
• inhibitory proteas krev   MeSH
• inhibitory trypsinu krev   MeSH
• komplementární DNA chemie   MeSH
• koncentrace vodíkových iontů MeSH
• krevní proteiny chemie   MeSH
• molekulární sekvence - údaje MeSH
• molekulová hmotnost MeSH
• neuraminidasa farmakologie   MeSH
• prasata MeSH
• sekvence aminokyselin MeSH
• sekvenční homologie MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• alfa-1-antichymotrypsin MeSH
• aminokyseliny MeSH
• chymotrypsin MeSH
• inhibitory proteas MeSH
• inhibitory trypsinu MeSH
• komplementární DNA MeSH
• krevní proteiny MeSH
• neuraminidasa MeSH