A simple and cheap all-in-one concept for at-line coupling of hollow fiber liquid-phase microextraction (HF-LPME) to commercial capillary electrophoresis (CE) is demonstrated, which enables the direct analysis of complex samples. A disposable microextraction device compatible with injection systems of Agilent CE instruments is proposed, which consists of a short segment of a porous HF attached to a tapered polypropylene holder. The holder maintains a constant position of the HF in a CE vial during extraction and simultaneously guides the injection end of a separation capillary into the HF lumen for automated CE injection and analysis. In a typical analytical procedure, the HF is impregnated with a water-immiscible solvent, its lumen is filled with 5 μL of an aqueous acceptor solution, and the microextraction device is placed in a 2 mL glass CE vial containing 550 μL of a donor solution. The vial is agitated at 750 rpm for 10 min, and the resulting acceptor solution is injected directly from the HF lumen into the commercial CE. No additional manual handling is required, except for the transfer of the CE vial to the CE autosampler. Multiple complex samples can be simultaneously pretreated in a multiple-well plate format, thus significantly reducing the total analysis time. Suitability of the analytical method is demonstrated by the direct determination of model basic drugs (nortriptyline, haloperidol, loperamide, and papaverine) in physiological solutions, urine, and dried blood spot (DBS) samples. Repeatability of the method is better than 12.8% (%RSD), extraction recoveries range between 34 and 76%, and enrichment factors are 37-84. The method is linear in a range of 2 orders of magnitude (R2 ≥ 0.9977) with limits of detection of 0.7-1.55 μg/L. The method has a high potential for the direct analysis of DBS samples since DBS elution and HF-LPME are performed simultaneously during the 10 min agitation. The manual DBS handling is thus reduced to inserting the DBS punch into the CE vial only. Moreover, the universal character of the HF-LPME might extend the applicability of the method to a wide range of analytes/matrices, and combination with other commercial detectors might improve the selectivity/sensitivity of the CE analysis.

• MeSH
• elektroforéza kapilární MeSH
• haloperidol analýza   MeSH
• lidé MeSH
• loperamid analýza   MeSH
• mikroextrakce kapalné fáze * MeSH
• nortriptylin analýza   MeSH
• papaverin analýza   MeSH
• poréznost MeSH
• povrchové vlastnosti MeSH
• tělesné tekutiny chemie   MeSH
• velikost částic MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• haloperidol MeSH
• loperamid MeSH
• nortriptylin MeSH
• papaverin MeSH

Microscopic colitis is characterized by chronic or intermittent watery diarrhoea. Microscopic colitis is a common cause of chronic diarrhoea in predominantly older adults. The underlying mechanism in the pathogenesis of microscopic colitis remains unspecified. Microscopic colitis including colitis collagenous, lymphocytic, microscopic colitis with incomplete findings, minimal change colitis, eosinophilic colitis, Brainerd´s diarrhoea, graft-versus-host disease, mastocytic enterocolitis and postinfectious irritable bowel syndrome. Careful consideration of the clinical features and colonic mucosal biopsies usually lead to correct diagnosis. Treatments of microscopic colitis were based primarily on case reports and personal experience. Many medications have been proposed that either offer symptomatic relief (loperamide, cholestyramine) or had anti-inflammatory or immunosuppressive properties (aminosalicylates, steroids, adalimumab, azathioprine).

• MeSH
• adalimumab MeSH
• antiflogistika terapeutické užití   MeSH
• azathioprin terapeutické užití   MeSH
• cholestyraminová pryskyřice terapeutické užití   MeSH
• eozinofilie komplikace   diagnóza   farmakoterapie   MeSH
• hormony kůry nadledvin terapeutické užití   MeSH
• humanizované monoklonální protilátky terapeutické užití   MeSH
• imunosupresiva terapeutické užití   MeSH
• kolitida kolagenní komplikace   diagnóza   farmakoterapie   MeSH
• kolitida lymfocytární komplikace   diagnóza   farmakoterapie   MeSH
• kolitida mikroskopická komplikace   diagnóza   farmakoterapie   MeSH
• kolitida komplikace   diagnóza   farmakoterapie   MeSH
• lidé MeSH
• loperamid terapeutické užití   MeSH
• mastocytóza komplikace   diagnóza   farmakoterapie   MeSH
• obstipancia terapeutické užití   MeSH
• průjem farmakoterapie   etiologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• adalimumab MeSH
• antiflogistika MeSH
• azathioprin MeSH
• cholestyraminová pryskyřice MeSH
• hormony kůry nadledvin MeSH
• humanizované monoklonální protilátky MeSH
• imunosupresiva MeSH
• loperamid MeSH
• obstipancia MeSH