The gene corresponding to the recently identified whiB-paralogous gene wblE in S. coelicolor was found after sequencing the downstream region of the stress-response sporulation-specific sigma-factor gene, sigH, in S. coelicolor A3(2). Sequence analysis has revealed an ORF exhibiting high similarity to sporulation transcription factors WhiB and WhiD. A stable null mutant of the wblE gene was obtained by integrative transformation, via double cross-over. Disruption of the S. coelicolor wblE gene appeared to have no obvious effect on growth, morphology, differentiation, and production of the pigmented antibiotics actinorhodin and undecylprodigiosin. Expression of the wblE gene was investigated during differentiation by S1 nuclease mapping, using RNA prepared from S. coelicolor A3(2) and its isogenic sigF and sigH mutants grown to various developmental stages. A single promoter was identified upstream of the wblE coding region. The wblEp promoter was induced at the beginning of aerial mycelium formation and its activity decreased later in differentiation. No differences in expression of the wblEp promoter were detected in S. coelicolor A3(2) mutants in sigF and sigH genes for sporulation-specific sigma factors. Sequence of the wblEp promoter showed partial similarity to the consensus sequence of the extracytoplasmic sigma factors.
- MeSH
- anthrachinony metabolismus MeSH
- bakteriální geny * MeSH
- bakteriální RNA izolace a purifikace MeSH
- DNA bakterií genetika izolace a purifikace MeSH
- inzerční mutageneze MeSH
- molekulární sekvence - údaje MeSH
- prodigiosin analogy a deriváty metabolismus MeSH
- promotorové oblasti (genetika) MeSH
- regulace genové exprese u bakterií MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- sekvenční homologie MeSH
- spory bakteriální * MeSH
- Streptomyces cytologie genetika růst a vývoj metabolismus MeSH
- transkripční faktory genetika fyziologie MeSH
- vývojová regulace genové exprese MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- actinorhodin MeSH Prohlížeč
- anthrachinony MeSH
- bakteriální RNA MeSH
- DNA bakterií MeSH
- prodigiosin MeSH
- transkripční faktory MeSH
- undecylprodigiosin MeSH Prohlížeč
