Microtubule organization and dynamics are critical for key developmental processes such as cell division, elongation, and morphogenesis. Microtubule severing is an essential regulator of microtubules and is exclusively executed by KATANIN 1 in Arabidopsis In this study, we comparatively studied the proteome-wide effects in two KATANIN 1 mutants. Thus, shotgun proteomic analysis of roots and aerial parts of single nucleotide mutant fra2 and T-DNA insertion mutant ktn1-2 was carried out. We have detected 42 proteins differentially abundant in both fra2 and ktn1-2 KATANIN 1 dysfunction altered the abundance of proteins involved in development, metabolism, and stress responses. The differential regulation of tubulins and microtubule-destabilizing protein MDP25 implied a feedback microtubule control in KATANIN 1 mutants. Furthermore, deregulation of profilin 1, actin-depolymerizing factor 3, and actin 7 was observed. These findings were confirmed by immunoblotting analysis of actin and by microscopic observation of actin filaments using fluorescently labeled phalloidin. Results obtained by quantitative RT-PCR analysis revealed that changed protein abundances were not a consequence of altered expression levels of corresponding genes in the mutants. In conclusion, we show that abundances of several cytoskeletal proteins as well as organization of microtubules and the actin cytoskeleton are amended in accordance with defective microtubule severing.
- MeSH
- aktiny metabolismus MeSH
- anotace sekvence MeSH
- Arabidopsis genetika metabolismus MeSH
- biologie buňky * MeSH
- genová ontologie MeSH
- katanin genetika MeSH
- mapy interakcí proteinů MeSH
- mikrotubuly metabolismus MeSH
- mutace genetika MeSH
- proteiny huseníčku genetika metabolismus MeSH
- proteom metabolismus MeSH
- proteomika metody MeSH
- rostlinné geny MeSH
- zpětná vazba fyziologická * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- aktiny MeSH
- katanin MeSH
- KTN1 protein, Arabidopsis MeSH Prohlížeč
- proteiny huseníčku MeSH
- proteom MeSH
