The widespread occurrence of cyanobacteria blooms damages the water ecosystem and threatens the safety of potable water and human health. Exogenous L-lysine significantly inhibits the growth of a dominant cyanobacteria Microcystis aeruginosa in freshwater. However, the molecular mechanism of how lysine inhibits the growth of M. aeruginosa is unclear. In this study, both non-target and target metabolomic analysis were performed to investigate the effects of algicide L-lysine. The results showed that 8 mg L- 1 lysine most likely disrupts the metabolism of amino acids, especially the arginine and proline metabolism. According to targeted amino acid metabolomics analysis, only 3 amino acids (L-arginine, ornithine, and citrulline), which belong to the ornithine-ammonia cycle (OAC) in arginine metabolic pathway, showed elevated levels. The intracellular concentrations of ornithine, citrulline, and arginine increased by 115%, 124%, and 19.4%, respectively. These results indicate that L-lysine may affect arginine metabolism and OAC to inhibit the growth of M. aeruginosa.
- Klíčová slova
- Arginine metabolism, L-lysine, Metabolomic analysis, Microcystin, Microcystis aeruginosa, Ornithine-ammonia cycle,
- MeSH
- amoniak MeSH
- arginin chemie metabolismus MeSH
- citrulin metabolismus MeSH
- ekosystém MeSH
- herbicidy * metabolismus MeSH
- lidé MeSH
- lysin toxicita metabolismus MeSH
- Microcystis * metabolismus MeSH
- mikrocystiny metabolismus MeSH
- ornithin toxicita metabolismus MeSH
- sinice * metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- amoniak MeSH
- arginin MeSH
- citrulin MeSH
- herbicidy * MeSH
- lysin MeSH
- mikrocystiny MeSH
- ornithin MeSH
